YARA ALZGHOUL, HALA J. BANI ISSA, AHMAD K. SANAJLEH, TAQWA ALABDUH, FATIMAH RABABAH, MAHA AL-SHDAIFAT, EJLAL ABU-EL-RUB^*, FATIMAH ALMAHASNEH, RAMADA R. KHASAWNEH, AYMAN ALZU’BI, HUTHAIFA MAGABLEH

BIOCELL, Vol.48, No.4, pp. 559-569, 2024, DOI:10.32604/biocell.2024.048056

Abstract Mesenchymal stem cells (MSCs) are ideal candidates for treating many cardiovascular diseases. MSCs can modify the internal cardiac microenvironment to facilitate their immunomodulatory and differentiation abilities, which are essential to restore heart function. MSCs can be easily isolated from different sources, including bone marrow, adipose tissues, umbilical cord, and dental pulp. MSCs from various sources differ in their regenerative and therapeutic abilities for cardiovascular disorders. In this review, we will summarize the therapeutic potential of each MSC source for heart diseases and highlight the possible molecular mechanisms of each source to restore cardiac function. More >

JING LI^1,2, WANWAN FAN⁴, LILI HAO¹, YONGSHENG LI⁵, GUOCHENG YU¹, WEI SUN⁶, XIANQIONG LUO^2,*, JINGXIANG ZHONG^1,3,*

BIOCELL, Vol.47, No.11, pp. 2485-2494, 2023, DOI:10.32604/biocell.2023.044177

Abstract Objective: This study aimed to investigate the potential of human umbilical cord mesenchymal stem cell (hucMSC)-derived exosomes (hucMSC-Exos) in inhibiting hypoxia-induced cell hyper proliferation and overexpression of vascular endothelial growth factor A (VEGF-A) in immature human fetal retinal microvascular endothelial cells (hfRMECs). Methods: Exosomes were isolated from hucMSCs using cryogenic ultracentrifugation and characterized through various techniques, including transmission electron microscopy, nanoparticle tracking analysis, bicinchoninic acid assays, and western blotting. The hfRMECs were identified using von Willebrand factor (vWF) co-staining and divided into four groups: a control group cultured under normoxic condition, a hypoxic model group, a hypoxic group treated with… More > Graphic Abstract

Xue Song^1,2, Cun Liu², Jingying Wang^1,*, Xinglian Yang¹, Mingrui Li¹

Molecular & Cellular Biomechanics, Vol.19, No.4, pp. 209-219, 2022, DOI:10.32604/mcb.2022.026082

Abstract The umbilical cord is a vital structure between the fetus and placenta for the growth and well-being of the fetus. Although the umbilical cord may be the only organ that dies at the beginning of life, it is one of the most important parts of the feta-placental unit and plays a role in determining how life begins. In general, the prenatal examination of the umbilical cord is limited to the observation of the number of vessels and the evaluation of umbilical artery blood flow parameters. Pathologists have done more research on the morphological characteristics of the umbilical cord and linked… More >

YUNLONG ZHANG^1,#, JINGWEI ZHANG^2,#, YU ZHU¹, BIN CAI^1,*

BIOCELL, Vol.46, No.5, pp. 1329-1338, 2022, DOI:10.32604/biocell.2022.015592

Abstract Diabetic patients often exhibit delayed or incomplete progress in the healing of acute wounds, owing to poor blood perfusion. Platelet-rich plasma (PRP) has attracted much attention as a means to improve wound healing, because it contains high growth factor concentrations. However, the burst-like release of PRP growth factors results in a short half-life of these therapeutic proteins, thus greatly limiting the therapeutic effect. In this study, we prepared PRP from human umbilical cord blood and developed an in situ photocrosslinkable PRP hydrogel glue (HNPRP) by adding a photoresponsive hyaluronic acid (HA-NB) into PRP. The HNPRP hydrogel allowed for controlled release… More >

MAKOTO NABETANI^1,*, TAKEO MUKAI²

BIOCELL, Vol.46, No.4, pp. 873-879, 2022, DOI:10.32604/biocell.2022.018218

Abstract Autism spectrum disorder (ASD) is a complex neurodevelopmental condition characterized by impairments in social communication, abnormal to absent verbal communication, the presence of repetitive stereotypic verbal and non-verbal behaviors and restricted interests, with onset early in life. We showed cognitive and behavioral characteristics of ASD by impairment of communication, cognition, perception, motor skills, executive function, theory of mind and emotion control. Recently, pathogenesis of immune pathology in the brains of individuals with ASD has been focused. New therapeutic approaches in the viewpoints of immune modulation and microglial function are logical for novel treatments for individuals with ASD. Cell therapies such… More >

JIGANG LEI^1,#, ZHENYAO XU^2,#, SUKE LI¹, MENG LI¹, ZHIKAI WANG³, PING LI¹, JING WANG¹, YINGLU CHEN¹, XIAOLE SONG¹, CHENGJIE REN¹, MEIPING SHEN¹, CHENGXIANG DAI^1,*

BIOCELL, Vol.45, No.3, pp. 537-546, 2021, DOI:10.32604/biocell.2021.014569

Abstract Psoriasis is an autoimmune-related chronic inflammatory disease with an approximate prevalence of 2–3% around the world, involving increased keratinocyte proliferation. Indeed, Th17 cells and IL-17 play critical roles in the pathogenesis of psoriasis. The monoclonal antibodies against cytokines have been shown to have effectively immunosuppressive effects on human psoriasis. However, there are still some patients that have no response to these treatments. Some patients have even serious side-effects which may affect their life. Mesenchymal stem cells have the ability of immunosuppressive and anti-inflammatory effects, which may be an alternative therapy with more safety and efficacy for human psoriasis. Moreover, the… More >

SHUCAI WU^1,#, DENGRUI LI^1,#, SUMIN GUO¹, LI GAO², YONGHUI YANG^1,*

BIOCELL, Vol.45, No.2, pp. 339-344, 2021, DOI:10.32604/biocell.2021.011379

Abstract Since stem cell therapy is the most effective treatment in the field of tissue reparation and reconstitution, the present study aimed to explore the different sources of mesenchymal stem cells (MSCs) on the different effects of pulmonary fibrosis-related cytokines in C57BL/6 mice. For reaching this goal, we isolated MSCs from umbilical cord blood and placenta and used for stem cell therapy in a mouse model of pulmonary fibrosis model. The pulmonary fibrosis model was done by injecting bleomycin into the trachea of C57BL/6 mice. Then we assessed the degree of pulmonary fibrosis in each mouse lung tissue at weeks 1,… More >

XUHE GONG¹, GUANGPU FAN², HUI LIU¹, SIJIA WANG³, RONGCHONG HUANG^1,*

BIOCELL, Vol.44, No.3, pp. 329-336, 2020, DOI:10.32604/biocell.2020.09639

Abstract This work aimed to study the beneficial role of human umbilical cord blood-derived mesenchymal stem cellconditioned medium (MSC-CM) in hypoxia-induced apoptosis in H9c2 cardiomyoblasts, in which the serine/heroine kinases (Akt) pathway would be involved. For this, CM was collected by culturing MSCs in serum-free DMEM medium for 24 h, and paracrine factors were analyzed by protein chip. H9c2 cells were divided into the following groups: control group, hypoxia group, MSC-CM intervention group (CM group), MSC-CM + Akt phosphorylation inhibitor (LY294002) group (LY group). Apoptosis of the H9c2 cells was tested with chromatin dye Hoechst 33342 and FITC-conjugated Annexin V apoptosis… More >

R. Canabarro^1,2, H. Sporleder², T. Gomes^3,4, G. Zanatta^3,5, L. Scribel^3,6, F. Freitas⁷, J. Neumann², P. Pranke^1,3

BIOCELL, Vol.31, No.3, pp. 397-403, 2007, DOI:10.32604/biocell.2007.31.397

Abstract The use of umbilical cord blood stem cells is an efficient alternative for the transplantation of hematopoietic progenitor cells. A number of factors can influence the volume and amount of CD34⁺ cells, which are considered as immature and capable of proliferation. Quantification of CD34⁺ cells, evaluation of CD38 and c-kit molecules on these cells, as well as correlations of such factors as maternal age, gestational age, newborn sex and weight, umbilical cord length, placental weight with increased volume and concentration of immature cells, among others, were performed in 70 blood samples from term newborns. The mean volume of umbilical cord… More >

Syed A Jamal^*

Molecular & Cellular Biomechanics, Vol.10, No.4, pp. 267-273, 2013, DOI:10.3970/mcb.2013.010.267

Abstract Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) were subjected to in vitro osteogenic differentiation using a novel combination of signaling molecules including BMP-2 and purmorphamine. Differentiation outcomes were assessed by calcein staining and by microscopic examination of the cytoskeleton. Calcein staining showed appreciable degree of calcium mineralization in cell culture, and changes in the morphological attributes of differentiating cells were observed vis-a-vis the actin cytoskeleton. Finally, positive calcein staining, altered cytoskeletal profile, and stress fiber formation in treated cells demonstrated, for the first time, a potentially synergistic interplay between BMP-2 and the hedgehog agonist, purmorphamine. This study lends support to… More >