LIJUN DU¹, KAIKAI DOU¹, NIANHAI LIANG^1,2, JIANMIN SUN¹, RU BAI^1,*

BIOCELL, Vol.47, No.3, pp. 547-554, 2023, DOI:10.32604/biocell.2023.025048

Abstract Background: The Warburg effect is considered as a hallmark of various types of cancers, while the regulatory mechanism is poorly understood. Our previous study demonstrated that miR-194-5p directly targets and regulates insulin-like growth factor1 receptor (IGF1R). In this study, we aimed to investigate the role of miR-194-5p in the regulation of the Warburg effect in ovarian cancer cells. Methods: The stable ovarian cell lines with miR-194-5p overexpression or silencing IGF1R expression were established by lentivirus infection. ATP generation, glucose uptake, lactate production and extracellular acidification rate (ECAR) assay were used to analyze the effects of aerobic glycolysis in ovarian cancer… More >

Jihong Sun^*†1, Jingjing Li^†1, Zhixian Guo^†, Lu Sun^†‡, Chenghui Juan^§, Yubing Zhou^¶, Hongli Gu^†, Yan Yu^†, Qiuyue Hu^†, Quancheng’ Kan^¶, Zujiang Yu^†

Oncology Research, Vol.27, No.4, pp. 407-417, 2019, DOI:10.3727/096504018X15180451872087

Abstract Most cancers rely disproportionately on glycolysis for energy even in the presence of an adequate oxygen supply, a condition known as “aerobic glycolysis,” or the “Warburg effect.” Pyruvate dehydrogenase E1 subunit (PDHA1) is one of the main factors for the metabolic switch from oxidative phosphorylation (OXPHOS) to aerobic glycolysis and has been suggested to be closely associated with tumorigenesis. Here we observed that the PDHA1 protein was reduced in hepatocellular carcinoma (HCC) specimens by immunohistochemistry and Western blot, which was significantly associated with poor overall survival. To further analyze the function of PDHA1 in cancer cells, PDHA1 was upregulated in… More >