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  • Open Access

    ARTICLE

    Boosting forage yield and quality of maize (Zea mays L.) with multi-species bacterial inoculation in Pakistan

    Iqbal A1, MA Iqbal1, A Iqbal1, Z Aslam1, M Maqsood1, Z Ahmad2, N Akbar1, HZ Khan1, RN Abbas1, RD Khan1, G Abbas1, M Faisal1

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 84-88, 2017, DOI:10.32604/phyton.2017.86.084

    Abstract Seed inoculation with bacterial species has the potential to increase yield and agro-qualitative attributes of forage crops. This study determined the response of forage maize to three plant growth promoting rhizobacteria [PGPR1 (Azotobacter chroococcum), PGPR2 (Pseudomonas flourescens) and PGPR3 (Bacillus megaterium)] inoculated individually and in different combinations (PGPR1+2, PGPR1+3, PGPR2+3 and PGPR1+2+3). A non-inoculated treatment was kept as a control. We used a completely randomized block design with four replicates. The PGPR1+2+3 treatment showed an outstanding performance by improving yield attributes, green forage yield, dry matter biomass, crude protein and total ash. The same treatment gave the lowest crude fiber… More >

  • Open Access

    ARTICLE

    Interaction of bifidobacteria with the gut and their influence in the immune function

    Gabriela Perdigón1,2, Mónica Locascio1, Marta Medici1, Aida Pesce de Ruiz Holagado1,2, Guillermo Oliver1

    BIOCELL, Vol.27, No.1, pp. 1-9, 2003, DOI:10.32604/biocell.2003.27.001

    Abstract Bifidobacteria are predominant in the lumen of the large intestine and confer various health benefits on the host. They are also used in the preparation of new fermented milks (bioyogurts) or added to conventional yogurt to generate probiotic effects. The colonization of the gut by bacteria tends to be host specific due partly to the way in which bacteria adhere to the intestinal wall. Using a homologous strain of Bifidobacterium animalis in an experimental mouse model, we analyzed by immunofluorescence labelledbacteria and transmission electronic microscopy the importance of this bacterial interaction with epithelial an immune cells associated to the gut,… More >

  • Open Access

    ARTICLE

    Antibacterial activity of lactose-binding lectins from Bufo arenarum skin

    A. Sánchez Riera, A. Daud, A. Gallo, S. Genta, M. Aybar, S. Sánchez

    BIOCELL, Vol.27, No.1, pp. 37-46, 2003, DOI:10.32604/biocell.2003.27.037

    Abstract Amphibians respond to microbial infection through cellular and humoral defense mechanisms such as antimicrobial protein secretion. Most humoral defense proteins are synthetized in the skin. In this study we isolated two β-galactoside-binding lectins with molecular weights of 50 and 56 KDa from the skin of Bufo arenarum. These lectins have significant hemagglutination activity against trypsinized rabbit erythrocytes, which was inhibited by galactose-containing saccharides. They are water-soluble and independent of the presence of calcium. The antimicrobial analysis for each lectin was performed. At µmolar concentration lectins show strong bacteriostatic activity against Gram negative bacteria (Escherichia coli K12 4100 and wild strains… More >

  • Open Access

    ABSTRACT

    Minireview: Pigmented corpuscles in the midgut gland of Pomacea canaliculata and other Neotropical apple-snails (Prosobranchia, Ampullariidae): A possible symbiotic association

    A. Castro-Vazquez, E.A. Albrecht, I.A. Vega, E. Koch, C. Gamarra-Luques

    BIOCELL, Vol.26, Suppl.S, pp. 101-109, 2002

    Abstract This article has no abstract. More >

  • Open Access

    ARTICLE

    Oxidative stress in Microcystis aeruginosa as a consequence of global climate change

    Marcelo HERNANDO1, Christian HOUGHTON1, Leda GIANNUZZI2, Bernd KROCK3, Darío ANDRINOLO2, Gabriela MALANGA4,*

    BIOCELL, Vol.40, No.1, pp. 23-26, 2016, DOI:10.32604/biocell.2016.40.023

    Abstract Cyanobacteria are phototrophic organisms with great ecological and economical importance. Species of the genus Microcystis are known for their potential ability to synthesize toxins, notably microcystins. There is a growing interest in the evaluation of oxidative stress in relation to the impact of global climate change on natural ecosystems in different trophic levels. Several studies have focused on the analysis of organismal responses to mitigate the damage by controlling the generation of reactive oxygen species. Variations in environmental factors caused by climate change generate a situation of oxidative damage in Microcystis aeruginosa as a direct or indirect consequence. In this… More >

  • Open Access

    ARTICLE

    Experimental evidence of soil bacteria abundance as the primary driver of rhizosphere priming effect

    Ma YP1,2,3#, ZJ Zhang1,2#, TQ Su1#, CA Busso4, ER Johnston5, XG Han1,6, XM Zhang2*

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 286-291, 2018, DOI:10.32604/phyton.2018.87.286

    Abstract Soil microbial communities are thought to be responsible for the rhizosphere priming effect (RPE). However, because soil microbial communities are comprised of diverse components, very little is known about which component plays the critical role. In this study, soybean and cottonwood were grown at two latitudinal locations with different temperature and light conditions in-situ. We quantified RPE using a natural δ13C method, and measured the abundance, richness and composition of bacteria and fungi communities with DNA-based molecular methods. Among all potential variables, including the three aforementioned indexes of bacteria and fungi communities and soil physicochemical and plant indexes, bacterial abundance… More >

  • Open Access

    ARTICLE

    Seed inoculation with Pseudomonas fluorescens and Pseudomonas syringae enhanced maize growth in a compacted saline-sodic soil

    Zafar-ul-Hye M1, A Nasir1, M Aon1, S Hussain1, M Ahmad2, I Naz1

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 25-31, 2018, DOI:10.32604/phyton.2018.87.025

    Abstract Abiotic stresses like salt stress and soil compaction are responsible for increased ethylene production which may adversely affect crop growth. A field experiment was conducted to evaluate the response of seed inoculation with ACC-deaminase containing rhizobacteria (Pseudomonas fluorescens and Pseudomonas syringae) in the presence of recommended or half of a recommended rate of inorganic fertilizers at different growth stages of fodder maize in a compacted saline-sodic soil. At both fertilizer rates, seed inoculation with P. fluorescens and P. syringae significantly improved all growth parameters over the control treatment. After 30, 60 and 75 days of sowing, treatment with recommended NPK… More >

  • Open Access

    ARTICLE

    Influence of Plant Growth Promoting Rhizobacterial Inoculation on Wheat Productivity Under Soil Salinity Stress

    Muhammad Zafar-ul-Hye1,*, Tariq Shahzad Bhutta1, Muhammad Shaaban1, Shahid Hussain1, Muhammad Farooq Qayyum1, Umar Aslam1 and Zahir Ahmad Zahir2

    Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 119-129, 2019, DOI:10.32604/phyton.2019.06570

    Abstract Soil salinity affects the growth and yield of crops. The stress of soil salinity on plants can be mitigated by inoculation of plant growth promoting bacteria (PGPR). The influence of PGPR inoculation on wheat (Triticum aestivum L.) crop productivity under salinity stress has not been properly addressed so far. Therefore, the present study was conducted to investigate the effects of various PGPR strains (W14, W10 and 6K; alone and combined) at several growth attributes of wheat plant under different soil salinity gradients (3, 6 and 9 dS m-1). The growth attributes of wheat (height, roots, shoots, spikes, grains quality, biological… More >

  • Open Access

    ARTICLE

    Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis

    Quezada-Rivera JJ1, RE Soria-Guerra2, FS Pérez-Juárez2, L Martínez-González2, SE Valdés- Rodríguez3, NL Vasco-Méndez1, JF Morales-Domínguez1

    Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 25-35, 2019, DOI:10.32604/phyton.2019.04549

    Abstract The use of antimicrobial peptides (AMPs) synthesized by bacteria (bacteriocins) is an alternative for combating multidrug resistant bacterial strains and their production by recombinant route is a viable option for their mass production. The bacteriocin E-760 isolated from the genus Enterococcus sp. has been shown to possess inhibitory activity against Gram-negative and Gram-positive bacteria. In this study, the expression of a chimeric protein coding for E-760 in the nucleus of C. reinhardtii was evaluated, as well as, its antibacterial activity. The synthetic gene E-760S was inserted into the genome of C. reinhardtii using Agrobacterium tumefaciens. A transgenic line was identified… More >

  • Open Access

    ABSTRACT

    in silico Method for the Identification of Mycobacterial sp. Potential Drug Targets

    Ashutosh Singh1, Shruti Mishra2, Dhwani Raghav1, Asheesh shanker1, Vinay Sharma1

    The International Conference on Computational & Experimental Engineering and Sciences, Vol.6, No.2, pp. 119-124, 2008, DOI:10.3970/icces.2008.006.119

    Abstract Drug resistance has increased the pace of war against the ever-growing challenge of mycobacterial infections particularly with the growing menace of tuberculosis (TB).Previous studies reported several essential and virulent genes of mycobacterium like virS gene and mymA operon[1] through experimental approaches. However, Post genomic approach applied for the identification of targets for tuberculosis which includes the comparison of Mycobacterium tuberculosis CDC1551 proteome against database of essential genes and proteome of Homo sapiens. A total of approx 4000 proteins were studied and compared and 19 proteins were found to possess potentiality to call as Targets. More >

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