Vital-López L, MA Cruz-Hernández, S Fernández-Dávila, A Mendoza-Herrera

Phyton-International Journal of Experimental Botany, Vol.85, pp. 210-217, 2016, DOI:10.32604/phyton.2016.85.210

Abstract Genetically modified crops could cause negative effects on bacterial communities. In this study, we compared the bacterial community structure of two maize cultivars to determine whether the transgenic cultivar exerts a negative effect on bacterial communities inhabiting the rhizosphere. Cultivars included the genetically modified maize (Zea mays), with the pat-gene conferring resistance to the herbicide glufosinate (synonym: L-phosphinothricin), and the hybrid, conventional maize. Metagenomic DNA was extracted from the rhizosphere of plants grown in a greenhouse. Single-strand conformation polymorphism, based on polymerase chain reaction amplifying a partial subunit rRNA gene was used to characterize and generate genetic profiles that corresponded… More >

Ruíz-Salazar R¹, JS Muruaga-Martínez², MLP Vargas-Vázquez², G Alejandre-Iturbide⁴, G Castañón-Nájera³, S Hernández-Delgado¹, N Almaraz-Abarca⁴, N Mayek-Pérez^1,5

Phyton-International Journal of Experimental Botany, Vol.85, pp. 184-193, 2016, DOI:10.32604/phyton.2016.85.184

Abstract In order to identify genes of resistance to four diseases that affect Phaseolus sp. [i.e, Common Bacterial Blight (CBB), Bean Common Mosaic Virus (BCMV), Bean Golden Mosaic Virus (BGMV) and angular leaf spot (ALS)], we amplified nine SCAR (Sequence Characterized Amplified Regions) markers: four linked to resistance to common blight, two for BCMV, two for BGMV and one for ALS. The genetic material included 16 ayocote bean (P. coccineus) populations collected from different locations at Veracruz and Puebla, Mexico. Populations from Veracruz showed greater number of SCARs for BGMV and ALS compared with populations from Puebla. The best P. coccineus… More >

Vital López L, MA Cruz Hernández, S Fernández Dávila, A Mendoza Herrera

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 233-243, 2015, DOI:10.32604/phyton.2015.84.233

Abstract Cultivated surfaces of genetically modified (GM) crops increased year by year, becoming in 2012 more extensive in developed than in industrialized countries. Furthermore, it has been postulated that the plant is which leads to the selection of the microorganisms on its root exudates, creating specific conditions which in turn regulate the specific microbial structure of each plant. In this study, our main objective was to examine whether the introduction of transgenic maize herbicide-tolerant plants will impact the microbial structures that inhabit at the rhizosphere and rhizoplane with respect to conventional hybrid maize plants. Bacterial populations were determined (CFU/g) using four… More >

Hernández-Castillo FD¹, RH Lira-Saldivar², G Gallegos-Morales¹, M Hernández-Suárez¹, S Solis-Gaona²

Phyton-International Journal of Experimental Botany, Vol.83, pp. 49-55, 2014, DOI:10.32604/phyton.2014.83.049

Abstract One of the most severe phytosanitary problems that face chili pepper producers in Mexico, and in many other parts of the world, is the disease known as "secadera" or wilting, caused by diverse pathogens. These patogens are mainly controlled with synthetic pesticides, thus causing a severe ecological impact, toxicity to humans, generation of plant resistance to fungicides, and increments of production costs. Because of this, it rises the need of finding more environmentally friendly options. We evaluated rhizospheric bacteria as a possible biological control of pepper wilt. We used three bacterial strains belonging to the Bacillus genera. These strains were… More >

Ruiz SE, AJ Cristóbal, RA Reyes, SJ Tun, RA García, AJ Pacheco

Phyton-International Journal of Experimental Botany, Vol.83, pp. 45-47, 2014, DOI:10.32604/phyton.2014.83.045

Abstract The antagonistic activity of native Bacillus subtilis strains from Yucatán peninsula soils were evaluated on two soilborne pathogens. Bacillus subtilis cbck36 and cbrf24 caused more than 60% inhibition of colony growth in Machophomina phaseolina. Cell-free culture filtrate of B. subtilis cbr24 were active against second- stage juveniles (J2) of Meloidogyne incognita (LC₅₀ 25.8% v/v). More >

Velázquez-Sepúlveda I, MC Orozco-Mosqueda, CM Prieto-Barajas, G Santoyo

Phyton-International Journal of Experimental Botany, Vol.81, pp. 81-87, 2012, DOI:10.32604/phyton.2012.81.081

Abstract Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gammaproteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they corresponded to… More >

Manilal¹ A, S Sujith², J Selvin³, C Shakir⁴, G Seghal Kiran⁵

Phyton-International Journal of Experimental Botany, Vol.78, pp. 161-166, 2009, DOI:10.32604/phyton.2009.78.161

Abstract The antibacterial property of the red algae, Falkenbergia hillebrandii (Born) collected from the southwest coast of India (Indian Ocean) was evaluated against three multidrug resistant human pathogens. Four different solvents: ethyl acetate, dichloromethane, methanol and phosphate buffer saline (PBS) were used with this purpose. Dried samples extracted with methanol showed broadest and highest antimicrobial activity when compared to other solvents. However, PBS extract showed no antibacterial activity. The highly active compounds red alga, F. hillebrandii were fractionated and purified using different chromatographic systems, including reverse phase HPLC and GCMS. The analysis revealed that the most abundant metabolite was the oleic… More >

Montenegro G, F Salas, RC Peña, R Pizarro

Phyton-International Journal of Experimental Botany, Vol.78, pp. 141-146, 2009, DOI:10.32604/phyton.2009.78.141

Abstract The detection of phenolics, flavonoids and related compounds by HPLC in extracts of unifloral honey of Quillay (Quillaja saponaria) showed phenolic compounds such as caffeic, coumaric and salicylic acids, the flavanone naringenin and the flavonol kaempferol. These extracts showed antibacterial activity against Pseudomonas aureginosa, Escherichia coli, Staphylococcus typhi, S. aureus, Streptococcus pneumoniae type β, and Vibrio cholerae, and antifungal activity against Candida albicans. Additionally, some aroma and decomposition compounds were identified in the ethanol extract. More >

Hernández-Castillo¹ FD, RH Lira-Saldivar², L Cruz-Chávez¹, G Gallegos-Morales¹, ME Galindo-Cepeda¹, E Padrón-Corral¹, M Hernández-Suárez²

Phyton-International Journal of Experimental Botany, Vol.77, pp. 241-252, 2008, DOI:10.32604/phyton.2008.77.241

Abstract Potato crop requires more fungicides than any other crop in Mexico to prevent and control several diseases. More than 21,3% of the total available fungicides are required for cropping potato. High quantities of synthetic pesticides are intended to control Phytophthora infestans and Rhizoctonia solani fungi. As a result, this produces severe health problems and ecosystem disturbances. There is then an urgent need for finding new options for sustainable management of potato crop diseases. Several experiments were conducted under laboratory, greenhouse, and field conditions, to: (1) analyze the antifungal effect of bacteria strains of the genus Bacillus, and their effect on… More >

Castillo-Aguilar C de la C¹, JJ Zúñiga-Aguilar², AA Guzmán-Antonio², R Garruña³

Phyton-International Journal of Experimental Botany, Vol.86, pp. 199-204, 2017, DOI:10.32604/phyton.2017.86.199

Abstract The Habanero pepper (Capsicum chinense) is intensively cultivated in the Yucatan peninsula, México. Because of adverse environmental conditions, it required seeding in germination trays, from which six-week-old seedlings were transplanted to the soil. Adequate nursing and fertilization programmes were made to improve health and vigour before seedlings transplanting. During seed germination, we investigated the effects of inoculation with four plant growth-promoting rhizobacteria (PGPR) on growth, nutrient uptake and gas exchange of 8-week-old Capsicum chinense plants. Inoculation was made with Pseudomonas sp. -P61-, Pseudomonas sp. -A46-, Bacillus pumillus -R44-, and Paenibacillus polymyxa-BSP1.1-. The BSP1.1 strain produced the highest increase in plant… More >