CHRISTINA LOH¹, YUQI ZHENG¹, ISLAM ALZOUBI², KIMBERLEY L. ALEXANDER^3,4, MAGGIE LEE⁴, WEI-DONG CAI², YANG SONG⁵, KERRIE MCDONALD⁶, ANNA K. NOWAK⁷, RICHARD B. BANATI^8,9, MANUEL B. GRAEBER^1,4,10,*

Oncology Research, Vol.33, No.4, pp. 937-950, 2025, DOI:10.32604/or.2024.056436 - 19 March 2025

Abstract Background: Microglia and brain macrophages contribute significantly to the tumor microenvironment in highly malignant glioblastoma where they are considered important drivers of tumor progression. A better understanding of the role of the brain macrophages present in glioblastoma appears crucial for improving therapeutic outcomes, especially in the context of novel immunotherapeutic approaches. Methods: We investigated the regulation of two well-established markers for microglia and brain macrophages, IBA1 and CD163, in relation to glioblastoma tumor necrosis using immunohistochemistry and modality fusion heatmaps of whole slide images obtained from adjacent tissue sections. Results: IBA1 and CD163 showed remarkable differences… More >

Syed Ijaz Ur Rahman¹, Naveed Abbas¹, Sikandar Ali², Muhammad Salman¹, Ahmed Alkhayat³, Jawad Khan^4,*, Dildar Hussain⁵, Yeong Hyeon Gu^5,*

CMES-Computer Modeling in Engineering & Sciences, Vol.142, No.2, pp. 1199-1231, 2025, DOI:10.32604/cmes.2025.057462 - 27 January 2025

Abstract Automatic detection of Leukemia or blood cancer is one of the most challenging tasks that need to be addressed in the healthcare system. Analysis of white blood cells (WBCs) in the blood or bone marrow microscopic slide images play a crucial part in early identification to facilitate medical experts. For Acute Lymphocytic Leukemia (ALL), the most preferred part of the blood or marrow is to be analyzed by the experts before it spreads in the whole body and the condition becomes worse. The researchers have done a lot of work in this field, to demonstrate… More >

YARA ALZGHOUL, HALA J. BANI ISSA, AHMAD K. SANAJLEH, TAQWA ALABDUH, FATIMAH RABABAH, MAHA AL-SHDAIFAT, EJLAL ABU-EL-RUB^*, FATIMAH ALMAHASNEH, RAMADA R. KHASAWNEH, AYMAN ALZU’BI, HUTHAIFA MAGABLEH

BIOCELL, Vol.48, No.4, pp. 559-569, 2024, DOI:10.32604/biocell.2024.048056 - 09 April 2024

Abstract Mesenchymal stem cells (MSCs) are ideal candidates for treating many cardiovascular diseases. MSCs can modify the internal cardiac microenvironment to facilitate their immunomodulatory and differentiation abilities, which are essential to restore heart function. MSCs can be easily isolated from different sources, including bone marrow, adipose tissues, umbilical cord, and dental pulp. MSCs from various sources differ in their regenerative and therapeutic abilities for cardiovascular disorders. In this review, we will summarize the therapeutic potential of each MSC source for heart diseases and highlight the possible molecular mechanisms of each source to restore cardiac function. More >

QINGJIAN HE¹, HUIXIN ZHU^2,3, SHANHONG FANG^4,5,*

BIOCELL, Vol.48, No.2, pp. 205-216, 2024, DOI:10.32604/biocell.2023.045109 - 23 February 2024

Abstract Introduction: Dexamethasone (Dex) caused impaired osteoblast differentiation and oxidative stress (OS) in bone marrow mesenchymal stem cells (BMSCs). This work sought to elucidate the precise molecular pathway through which Dex influences osteogenic differentiation (OD) and OS in BMSCs. Methods: The expression of Runt-related transcription factor 1 (RUNX1) and alpha-2 macroglobulin (A2M) was assessed in Dex-treated BMSCs using qRT-PCR and Western Blot. Following the functional rescue experiments, cell proliferation was determined by MTT assay, reactive oxygen species (ROS) expression by DCFH-DA fluorescent probe, lactate dehydrogenase (LDH), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (Gpx) expression… More >

MONA S. ABDELLATEIF^1,*, NAGLAA M. HASSAN², MAHMOUD M. KAMEL², YOMNA M. EL-MELIGUI²

Oncology Research, Vol.32, No.3, pp. 577-584, 2024, DOI:10.32604/or.2023.043026 - 06 February 2024

Abstract Background: microRNA-34a (miR-34a) had been reported to have a diagnostic role in acute myeloid leukemia (AML). However, its value in the bone marrow (BM) of AML patients, in addition to its role in response to therapy is still unclear. The current study was designed to assess the diagnostic, prognostic, and predictive significance of miR-34a in the BM of AML patients. Methods: The miR-34a was assessed in BM aspirate of 82 AML patients in relation to 12 normal control subjects using qRT-PCR. The data were assessed for correlation with the relevant clinical criteria, response to therapy,… More >

JINJIN FU^1,#, XIAOYUE FENG^2,#, JUAN WEI², XIANG GENG¹, YU GONG¹, FENGDONG LI¹, SHAOHUA ZHUANG¹, JIN HUANG¹, FANGYU WANG^2,*

BIOCELL, Vol.47, No.12, pp. 2627-2639, 2023, DOI:10.32604/biocell.2023.042586 - 27 December 2023

Abstract Background: This study aimed to elucidate the potential mechanisms through which bone marrow-derived mesenchymal stem cells (BM-MSCs) may be effective in alleviating experimental colitis induced by treatment with 2,4,6-trinitrobenzene-sulfonate acid (TNBS), specifically through autophagy modulation. Methods: BM-MSCs were collected from BALB/c mice for subsequent experiments. The study employed cell counting kits (CCK-8) to investigate the impact of the MSC-conditioned medium (M medium) on the proliferation of RAW264.7 macrophages. The GFP-mRFP-LC3 adenovirus was transfected into RAW264.7 to detect autophagic flux. The gene expression of cytokines was assessed through quantitative reverse transcription polymerase chain reaction (qRT-PCR). Western blot… More >

WENQI CHEN^1,#, XIAOYANG CHU^2,#, YANG ZENG^3,#, YOUSHENG YAN⁴, YIPENG WANG⁴, DONGLAN SUN¹, DONGLIANG ZHANG⁵, JING ZHANG^1,*, KAI YANG^4,*

BIOCELL, Vol.47, No.7, pp. 1561-1569, 2023, DOI:10.32604/biocell.2023.028851 - 21 June 2023

Abstract Background: Receptor tyrosine kinase-like orphan receptor 2 (ROR2) has a vital role in osteogenesis. However, the mechanism underlying the regulation of ROR2 in osteogenic differentiation is still poorly comprehended. A previous study by our research group showed that a novel compound heterozygous ROR2 variation accounted for the autosomal recessive Robinow syndrome (ARRS). This study attempted to explore the impact of the ROR2: c.904C>T variant specifically on the osteogenic differentiation of BMSCs. Methods: Coimmunoprecipitation (CoIP)-western blotting was carried out to identify the interaction between ROR2 and Wnt5a. Double-immunofluorescence staining was used for determining the expressions and co-localization… More > Graphic Abstract

FRANCISCO RAÚL BORZONE^1,*, MARÍA BELÉN GIORELLO¹, LEANDRO MARCELO MARTINEZ², MARÍA CECILIA SANMARTIN^1,3, LEONARDO FELDMAN⁴, FEDERICO DIMASE⁵, EMILIO BATAGELJ⁶, GUSTAVO YANNARELLI³, NORMA ALEJANDRA CHASSEING^1,*

Oncology Research, Vol.31, No.3, pp. 361-374, 2023, DOI:10.32604/or.2023.028104 - 22 May 2023

Abstract Breast cancer is the predominant form of carcinoma among women worldwide, with 70% of advanced patients developing bone metastases, with a high mortality rate. In this sense, the bone marrow (BM) mesenchymal stem/stromal cells (MSCs) are critical for BM/bone homeostasis, and failures in their functionality, transform the BM into a pre-metastatic niche (PMN). We previously found that BM-MSCs from advanced breast cancer patients (BCPs, infiltrative ductal carcinoma, stage III-B) have an abnormal profile. This work aims to study some of the metabolic and molecular mechanisms underlying MSCs shift from a normal to an abnormal profile… More >

NINGYU LI^1,2,#, XIAOFANG CHEN^2,#,§, SUXIA GENG², PEILONG LAI², LISI HUANG², MINMING LI², XIN HUANG², CHENGXIN DENG², YULIAN WANG², JIANYU WENG², XIN DU^1,2,*

BIOCELL, Vol.47, No.1, pp. 133-141, 2023, DOI:10.32604/biocell.2022.022021 - 26 September 2022

Abstract The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. In this study, exosomes from bone marrow plasma were successfully extracted and identified. Assessment of miR-103-3p expression in exosomes isolated from BM in 34 MDS patients and 10 controls revealed its 0.52-fold downregulation in patients with MDS compared with controls (NOR) and was downregulated 0.55-fold in MDS-MSCs compared with NOR-MSCs. Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation More >

Lilan Chen, Lu Lu, Xinlei Gong, Yichen Xu, Xiaoyuan Chu^*, Guichun Huang^*

Oncologie, Vol.24, No.3, pp. 599-604, 2022, DOI:10.32604/oncologie.2022.023310 - 19 September 2022

Abstract Gastric cancer is the fifth most commonly diagnosed cancer and the fourth leading cause of cancer death worldwide in 2020. Gastric cancer usually undergoes lymph node metastasis and implantation metastasis, but bone metastasis and bone marrow invasion are rare. However, gastric cancer patients with bone marrow invasion usually have cancer emergency, so special attention should be paid in clinical practice. Herein, we analyzed the clinical characteristics of an asian gastric cancer patient with bone marrow invasion and disseminated intravascular coagulation (DIC) in our hospital and summarized the diagnosis and treatment experience to provide a reference More >