TINGTING HUANG^1,#, SHAN DENG^2,#, JIANGYUAN SHENG¹, DI ZHANG^1,*

BIOCELL, Vol.46, No.11, pp. 2507-2515, 2022, DOI:10.32604/biocell.2022.021314

Abstract Dehydrins (DHNs), as members of the late embryogenesis abundant protein family, play critical roles in the protection of seeds from dehydration and plant adaptation to multiple abiotic stresses. Vitrification is a basic method in plant cryopreservation and is characterized by forming a glassy state to prevent lethal ice crystals produced during cryogenic storage. In this study, ApSK₃ type DHN was genetically transformed into embryogenic calluses (EC) of Agapanthus praecox by overexpression (OE) and RNA interference (RNAi) techniques to evaluate the in vivo protective effect of DHNs during cryopreservation. The cell viability showed a completely opposite trend in OE and RNAi… More >

Xiaotong Wei^1,#, Xuhong Fan^2,#, Xuan Lu¹, Liyuan Han¹, Honglin Zhang³, Siyan Liu³, Jing Qu³, Shuyan Guan^3,*, Yiyong Ma^3,*

Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1945-1957, 2022, DOI:10.32604/phyton.2022.020062

Abstract

The embryos of maize (Zea mays L.) inbred lines GS02, GS07, GS08, GS11 and GS15 were used as receptor materials to optimize the receptor system from the aspects of genotype, medium components and stress (PEG6000, mannitol, salt and low phosphorus). The results showed that GS07 had the highest induction rate (95.2%). Orthogonal test analysis showed that the best combination of medium components in induction was A₂B₃C₁D₃₍₂₎, namely, the concentration of 2, 4-dichlorophenoxy acetic acid (2,4-D) was 4 mg·mL^-1, the concentration of L-Proline (L-Pro) was 0.8 mg·mL^-1, and the concentration of silver nitrate (AgNO₃) was 10 mg·mL^-1 (or 5 mg·mL). Interestingly,… More >

SITI NABILAH^1,2; TRI HANDOYO^2,3; KYUNG-MIN KIM⁴; MOHAMMAD UBAIDILLAH^2,*

BIOCELL, Vol.46, No.7, pp. 1633-1641, 2022, DOI: 10.32604/biocell.2022.019111

Abstract Somatic embryogenesis is an asexual reproduction process that occurs in many plant species, including rice. This process contains several totipotency markers such as Somatic Embryogenesis Receptor-like Kinase (SERK), Leafy Cotyledon1 (LEC1) and WUSCHEL-Related Homeobox4 (WOX4) and also a helpful model for embryo development and clones and transformations. Here, we report the gene expression during somatic embryo development correlates with regeneration frequency in 14 Javanica rice (pigmented and non-pigmented) using modifified N6 media supplemented with Kinetin (2.0 mg/L) and NAA (1.0 mg/L). Although there have been advances in understanding the genetic basis of somatic embryogenesis in other varieties, rice is still… More >

Yingbo Li^1,2,#, Ting He^1,2,#, Guimei Guo^1,2, Hongwei Xu^1,2, Yingjie Zong^1,2, Shuwei Zhang^1,2, Ruiju Lu^1,2, Longhua Zhou^1,2,*, Chenghong Liu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1153-1164, 2022, DOI:10.32604/phyton.2022.019520

Abstract Agrobacterium-mediated transformation has been widely used in plants. However, the mechanism in plant cells’ response to Agrobacterium infection was very complex. The mechanism of the determinants in host cell remains obscure, especially in barley, which is recalcitrant for Agrobacterium-mediated transformation. In the present study, microspore-derived embryogenic calli (MDEC) from barley elite cultivar were employed as unique subjects to characterize the mechanisms during the Agrobacterium infection process. Hua 30 MDEC can be successfully infected by Agrobacterium. RNA-sequencing at different infection points (0, 2, 6, 12, 24 hpi) was performed. The average expressional intensity of the whole genomics increased from 0 to… More >

Yinkai Xi^1,2, Biao Zeng³, Hengyu Huang^1,4,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 987-1001, 2021, DOI:10.32604/phyton.2021.014218

Abstract An efficient regeneration system of Rhynchostylis retusa was established to provide technical reference for the application of tissue culture tube seedlings in production. The mixtures of callus and protocorm from aseptic germination were used as explants. The optimal media of each stage was selected for callus proliferation, protocorm occurrence and growth, rejuvenation and rooting via a single, complete combination and orthogonal experiment. The results showed that the optimal medium for callus proliferation, protocorms occurrence and growth was 1/2 Murashige and Skoog (MS) medium adding 50 g·L⁻¹ banana puree, 0.1 mg·L⁻¹ α-naphthaleneacetic acid (NAA), 1.5 mg·L⁻¹ 6-benzylaminopurine (6-BA) and 1.0 mg·L⁻¹… More >

Lucía Isabel Chávez-Ortiz¹, José Francisco Morales-Domínguez¹, Araceli Rodríguez-Sahagún², Eugenio Pérez-Molphe-Balch^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 1003-1013, 2021, DOI:10.32604/phyton.2021.012862

Abstract Agave guiengolaGentry is an endemic plant from a very small locality in Oaxaca, Mexico. Its conservation status is fragile and can rapidly worsen. Because of its scarcity, this agave has been used solely for ornamental purposes, but it could have other uses if more plants were available. In vitro propagation by enhanced axillary sprouting from stem segments was attained using Murashige and Skoog Basal Medium (MS) as well as basal medium supplemented with cytokinins 6-Benzylaminopurine (BA) or 6-(γ,γ-Dimethylallylamino)purine (2iP). The best treatment for shoot induction in semisolid medium consisted in MS supplemented with 2 mg l^–1 BA, obtaining a mean… More >

Anna Stepanova^1,*, Maria Malunova¹, Svetlana Salamaikina¹, Renat Selimov², Aleksandra Solov’eva¹

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 543-552, 2021, DOI:10.32604/phyton.2021.013223

Abstract Rhodiola quadrifida is a rare mountain medicinal plant whose root extracts are used in traditional Chinese medicine as a hemostatic, antitussive, and tonic in the treatment of gynecological diseases. The aim of the study was to obtain R. quadrifida cultures at different degrees of differentiation in vitro and compare their growth characteristics and the content of salidroside and rosavin. Hairy roots were obtained by incubating cotyledons and hypocotyls in a suspension of Agrobacterium rhizogenes strain A4. The presence of the rolB and rolC genes was proven by polymerase chain reaction. The obtained roots were cultivated in Murashige-Skoog medium (MS). Calluses… More >

Peng Jiao^1,#, Ruiqi Ma^2,#, Zhuo Qi¹, Zhenzhong Jiang¹, Siyan Liu¹, Jing Qu³, Shuyan Guan^1,*, Yiyong Ma^3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 121-130, 2020, DOI:10.32604/phyton.2020.07980

Abstract This research uses the immature embryos of inbred maize lines (GSH9901, Hi01, Hi02, and Chang 7-2) as receptor materials to establish the callus induction system. These inbred lines provide the receptor materials for the genetic regeneration of maize and the verification of the genetic functions of maize. The factor experiment and orthogonal experiments were used to investigate the impacts of different genotypes, immature embryo size, shield orientation, 2, 4-D concentration, proline concentration, and folic acid concentration on the induction rate of embryogenic callus tissue. A sensitivity experiment testing glyphosate (Bar) and an antibiotic (Cefotaxime sodium) were also conducted. The results… More >

Paul Chege^1,2, Andrea Palágyi², Csaba Lantos², Erzsébet Kiss¹, János Pauk^2,*

Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 111-119, 2020, DOI:10.32604/phyton.2020.07554

Abstract Many attempts on optimization of sorghum [Sorghum bicolor (L.) Moench] tissue culture induction media have been made, but the culture system remains with some bottlenecks compared to that of other crops. This study aimed at assessing the suitability of various induction media to produce embryogenic callus (yellow and friable) with high induction rates and reduced phenolic exudation. The six culture medium modifications: 3 based on Murashige and Skoog (MS) medium and one each based on Chu N6, Gamborg B5 and 190-2 media respectively were applied in the culture of mature embryos from 10 sorghum genotypes. Although there was a genotype… More >

A. C. PONTAROLI, E. L. CAMADRO

BIOCELL, Vol.29, No.3, pp. 313-317, 2005, DOI:10.32604/biocell.2005.29.313

Abstract Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg.l^-1 kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg.l^-1 2,4-D and 1 mg.l^-1 kinetin. Shoot primordia (SP) induction from >18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were obtained… More >