Xuesong Wang^1,#, Zhihong Wang^1,2,#, Qiuling Yang¹, Sheng Peng³, Mijun Peng^1,*

Journal of Renewable Materials, Vol.10, No.12, pp. 3131-3145, 2022, DOI:10.32604/jrm.2022.020204

Abstract To evaluate the potential anti-aging ability of Eucommia ulmoides, four characteristic components (chlorogenic acid, geniposidic acid, aucubin, quercetin) were selected to assess their effects on H2O2-induced oxidative damage model of human umbilical vein endothelial cell (HUVEC). Oxidative damage indexes, inflammatory factors, cell cycle, cell apoptosis, cell senescence, and their related proteins were analyzed by methyl thiazolyl tetrazolium (MTT) assay, enzyme-linked immunosorbent assay (ELISA), propidium iodide (PI) staining, annexin V-FITC/ PI double staining, SA β-galactosidase staining, and western blotting (WB). The results showed that H2O2- induced cell growth inhibition rate decreased as supplementation with characteristic components when compared to H2O2 group.… More >

LI KAILONG^1,2, DU XIAOLAN¹, HE YANI², ZHAO LIN¹, YANG JVRONG², SONG RUIHUA¹, CHEN LIN¹

BIOCELL, Vol.31, No.2, pp. 213-223, 2007, DOI:10.32604/biocell.2007.31.213

Abstract Objective: To investigate the course of tubular cell senescence and expressions of p53, p21, and Rb during the late phase of ischemia/reperfusion (IRI) in the kidney, and assess the effects of the p53-Rb pathway on tubular cell senescence. Methods: Experimental models of unilateral renal IRI were used in p53(+/+) and p53(-/-) mice. Histological changes at the tubular level, progress of cell senescence, and the expression of Rb, p21, and/or p53 proteins in tubular cells were studied at different moments in time after IRI. Results: Chronic tubulointerstitial fibrosis was much more severe and widely distributed in IRI kidneys of p53(+/+) mice… More >