Maria Babar¹, Siddra Ijaz^1,*, Imran Ul Haq², Muhammad Sarwar Khan¹

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1289-1300, 2023, DOI:10.32604/phyton.2023.026469

Abstract We developed a molecular marker for MAS of mungbean resistant varieties against CLS from the consensus sequence (MB-CLsRG) of identified RGAs (MB-ClsRCaG1 and MB-ClsRCaG2). The MB-CLsRG sequence-specific primer pair was used to screen Cercospora leaf spot (CLS) resistant varieties of mungbean in genomic analysis that showed congruency with phenotypic screening. Validation of molecular marker linkage with CLS resistance was performed using rtPCR in transcriptomic analysis. The sequenced PCR products showed 100% homology with MB-CLsRG sequence and putative disease resistance proteins that confirmed the linkage of molecular marker with CLS resistance in mungbean. The antifungal potential of MB-CLsRG gene encoding protein… More >

Yongjing Xi, Zhou Yang, Yukun Jin, Jing Qu, Shuyan Guan, Siyan Liu, Piwu Wang^*

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1257-1274, 2023, DOI:10.32604/phyton.2023.025918

Abstract PCR detection, quantitative real-time PCR (q-RTPCR), outdoor insect resistance, and disease resistance identification were carried out for the detection of genetic stability and disease resistance through generations (T₂, T₃, and T₄) in transgenic maize germplasms (S3002 and 349) containing the bivalent genes (insect resistance gene Cry1Ab13-1 and disease resistance gene NPR1) and their corresponding wild type. Results indicated that the target genes Cry1Ab13-1 and NPR1 were successfully transferred into both germplasms through tested generations; q-PCR confirmed the expression of Cry1Ab13-1 and NPR1 genes in roots, stems, and leaves of tested maize plants. In addition, S3002 and 349 bivalent gene-transformed lines… More >

Adel A. Rezk^1,2, Mohamed M. El-Malky³, Hossam S. El-Beltagi^1,4,*, Mohammed Al-daej¹, Kotb A. Attia^5,6

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 725-746, 2023, DOI:10.32604/phyton.2022.024645

Abstract Monogenic lines, which carried 23 genes for blast resistance were tested and used donors to transfer resistance genes by crossing method. The results under blast nursery revealed that 9 genes from 23 genes were susceptible to highly susceptible under the three locations (Sakha, Gemmeza, and Zarzoura in Egypt); Pia, Pik, Pik-p, Piz-t, Pita, Pi b, Pi, Pi 19 and Pi 20. While, the genes Pii, Pik-s, Pik-h, Pi z, Piz-5, Pi sh, Pi 3, Pi 1, Pi 5, Pi 7, Pi 9, Pi 12, Pikm and Pita-2 were highly resistant at the same locations. Clustering analysis confirmed the results, which… More >

M. Ashraf Ahangar¹, Shabir Hussain Wani^1,*, Zahoor A. Dar², Jan Roohi¹, Fayaz Mohiddin¹, Monika Bansal³, Mukesh Choudhary⁴, Sumit K. Aggarwal⁴, S. A. Waza¹, Khursheed Ahmad Dar⁵, Ayman El Sabagh^6,7, Celaleddin Barutcular⁸, Omer Konuşkan⁹, Mohammad Anwar Hossain^10,*

Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2111-2133, 2022, DOI:10.32604/phyton.2022.020721

Abstract Maize is cultivated extensively throughout the world and has the highest production among cereals. However, Northern corn leaf blight (NCLB) disease caused by Exherohilum turcicum, is the most devastating limiting factor of maize production. The disease causes immense losses to corn yield if it develops prior or during the tasseling and silking stages of crop development. It has a worldwide distribution and its development is favoured by cool to moderate temperatures with high relative humidity. The prevalence of the disease has increased in recent years and new races of the pathogen have been reported worldwide. The fungus E. turcicum is… More >

Huimin Cui, Shuo Qu, Abraham Lamboro, Yaolei Jiao, Piwu Wang^*

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1445-1464, 2022, DOI:10.32604/phyton.2022.020010

Abstract In agricultural production, a single insect-resistant and disease-resistant variety can no longer meet the demand. In this study, the expression vector pCAMBIA-3301-PR1 containing the disease-resistant gene PR1 was constructed by means of genetic engineering, and the PR1 gene was genetically transformed to contain the PR1 gene through the pollen tube method. In CryAb-8Like transgenic high-generation T₇ receptor soybean, a new material that is resistant to insects and diseases is obtained. For T₂ transformed plants, routine PCR detection, Southern Blot hybridization, fluorescence quantitative PCR detection, indoor and outdoor pest resistance identification and indoor disease resistance identification were performed. The results showed… More >

Huiyun Li, Ye Liu, Sumei Chen, Jiafu Jiang, Aiping Song, Weimin Fang, Fadi Chen^*

Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 851-859, 2020, DOI:10.32604/phyton.2020.012361

Abstract Black spot disease, caused by the necrotrophic fungus Alternaria tenuissima (Fr.) Wiltsh (A. tenuissima), is considered a highly destructive disease of Chrysanthemum (Chrysanthemum morifolium Ramat.). A set of 17 accessions of commercial chrysanthemum cultivars were evaluated for resistance to A. tenuissima by seedling artificial inoculation. It was found that the reaction of the accessions to artificial inoculation ranged from resistant to highly susceptible. Five varieties of chrysanthemum (‘Zhongshan Taogui’, ‘Jinba’, ‘Zhongshan Jinguan’, ‘Jinling Wanhuang’ and ‘Jinling Yangguang’) were resistant; two varieties of chrysanthemum (‘Zhongshan Xinggui’ and ‘Zhongshan Jinkui’) were moderately resistant; and others were susceptible to various degrees, four varieties… More >

MST FARHANA NAZNEEN CHOWDHURY¹, MOHD RAFII YUSOP^1,2,*, SITI IZERA ISMAIL³, SHAIRUL IZAN RAMLEE¹, YUSUFF OLADOSU², MONIR HOSEN², GOUS MIAH⁴

BIOCELL, Vol.44, No.3, pp. 269-278, 2020, DOI:10.32604/biocell.2020.09627

Abstract Chili (Capsicum annuum L.) is the popular spicy vegetable crops belonging to family Solanaceae. Chili peppers are known for their pungency characteristic due to the presence of capsaicinoids that classifies them into hot or sweet pepper. Chili is used as spices, folk remedies for diseases, vegetables, and coloring agent showing a diverse role in human’s life. However, its production is hampered by different biotic stress and abiotic factors. Similarly, the unavailability of high yielding varieties, high temperature, and disease incidence, particularly, anthracnose disease, are the major constraints responsible for the low production of chili pepper. The advents of molecular markers,… More >

Ning Xuan¹, Hua Zhang¹, Xu Liu¹, Qiang Guo¹, Jian Liu², Fangyin Yao^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 45-55, 2020, DOI:10.32604/phyton.2020.08548

Abstract Rice blast disease is one of the most devastating diseases of rice (Oryza sativa L.) caused by the fungus Magnaporthe oryzae (M. oryzae), and neck blast is the most destructive phase of this illness. The underlying molecular mechanisms of rice blast resistance are not well known. Thus, we collected 150 rice varieties from different ecotypes in China and assessed the rice blast resistances under the natural conditions that favoured disease development in Jining, Shandong Province, China in 2017. Results showed that 92 (61.3%) and 58 (38.7%) rice varieties were resistant and susceptible to M. oryzae, respectively. Among the 150 rice… More >

Ruíz-Salazar R¹, JS Muruaga-Martínez², MLP Vargas-Vázquez², G Alejandre-Iturbide⁴, G Castañón-Nájera³, S Hernández-Delgado¹, N Almaraz-Abarca⁴, N Mayek-Pérez^1,5

Phyton-International Journal of Experimental Botany, Vol.85, pp. 184-193, 2016, DOI:10.32604/phyton.2016.85.184

Abstract In order to identify genes of resistance to four diseases that affect Phaseolus sp. [i.e, Common Bacterial Blight (CBB), Bean Common Mosaic Virus (BCMV), Bean Golden Mosaic Virus (BGMV) and angular leaf spot (ALS)], we amplified nine SCAR (Sequence Characterized Amplified Regions) markers: four linked to resistance to common blight, two for BCMV, two for BGMV and one for ALS. The genetic material included 16 ayocote bean (P. coccineus) populations collected from different locations at Veracruz and Puebla, Mexico. Populations from Veracruz showed greater number of SCARs for BGMV and ALS compared with populations from Puebla. The best P. coccineus… More >

Giménez¹ C, E de García², O Haddad³

Phyton-International Journal of Experimental Botany, Vol.77, pp. 65-79, 2008, DOI:10.32604/phyton.2008.77.065

Abstract Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through in vitro process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from vitroplants randomly selected) with… More >