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  • Open Access


    Interaction of Acaena elongata L. with Arbuscular Mycorrhizal Fungi under Phosphorus Limitation Conditions in a Temperate Forest

    Yasmin Vázquez-Santos1, Yuriana Martínez-Orea1, Javier Álvarez-Sánchez1, Noé Manuel Montaño2, Sara Lucía Camargo-Ricalde2, Silvia Castillo-Argüero1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 605-619, 2021, DOI:10.32604/phyton.2021.014208

    Abstract The aim of this study was to analyze the performance of Acaena elongata colonized by arbuscular mycorrhizal fungi (AMF) to different phosphorus (P) concentrations, as a measure of AMF dependency. A. elongata, is a species from soils where P availability is limited, such as temperate forests. Our research questions were: 1) How do different P concentrations affect the AMF association in Acaena elongata, and 2) How does the AMF association influence A. elongata’s growth under different P concentrations? A. elongata’s growth, P content in plant tissue, AMF colonization and dependency were measured under four P concentrations: control (0 g P… More >

  • Open Access


    Gene expression of granulosa and cumulus cells: The prospect in predicting the quality and developmental competence of oocytes in vitro maturation


    BIOCELL, Vol.44, No.4, pp. 487-499, 2020, DOI:10.32604/biocell.2020.011638

    Abstract In vitro maturation (IVM), a promising assisted reproductive technology (ART), has been evolving in clinical trials and applications. There is a huge potential demand for IVM in clinical practice because it reduces the stimulation of gonadotropins to patients and provides evidence for the safety of neonatal birth. Unfortunately, the maturation rate of oocytes in vivo is not as high as it is in vivo due to a different microenvironment. Moreover, there are still controversies in predicting the developmental capability of oocytes in IVM. The granulosa cells (GCs) and cumulus cells (CCs), closely surrounding the oocytes, play a critical role in… More >

  • Open Access


    Single-channel response of hamster oocytes to fertilization with homologous spermatozoa


    BIOCELL, Vol.30, No.1, pp. 43-49, 2006, DOI:10.32604/biocell.2006.30.043

    Abstract Electrophysiological events occur early after fertilization, along with changes in intracellular Ca2+ concentration . Passive electrical parameters were determined in golden hamster oocytes by whole cell patch-clamp method. In separate experiments the effect of 4-aminopyridine on resting oocytes was tested. The single-channel patch clamp configuration was employed to assess the electrical response to fertilization with homologous sperm. Structure of oocytes submitted to patch clamp was evaluated with scanning electron microscopy and found to be preserved.
    Oocyte diameter was 70.2 ± 2.2 µm; their resting parameters were: membrane potential 23.8 ± 0.8 mV; total membrane specific resistance 519.1 ± 94.6… More >

  • Open Access


    Quality and capsaicinoid concentration on genotypes of Serrano pepper (Capsicum annuum L.) produced under organic fertilization

    Valadez Sánchez YM1, E Olivares Sáenz1, RE Vázquez Alvarado1, JR Esparza-Rivera3, P Preciado-Rangel4, RD Valdez-Cepeda5, JL García-Hernandez2

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 21-26, 2016, DOI:10.32604/phyton.2016.85.021

    Abstract The aim of this study was to evaluate the effect of three organic fertilization sources on the agronomic and biochemical qualities of four genotypes of Serrano pepper. The experiment was performed at the field in Gómez Palacio, Durango (México). The capsaicinoids (CAP´s) are compounds responsible of the pungency of the peppers, and they are valuable for health and food and cosmetic food. The organic treatments applied were: solarized bovine manure (60 Mg/ha), vermicompost (10 Mg/ha) and poultry manure (5 Mg/ ha). A non-fertilized treatment was used as control. The evaluated variables were: length, diameter and individual weight on fruits, and… More >

  • Open Access


    Use of diammonium phosphate on wheat grown in southwestern Buenos Aires (Argentina)

    Ron MM, T Loewy

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 15-20, 2016, DOI:10.32604/phyton.2016.85.015

    Abstract In the southwest of Buenos Aires Province (Argentina), nitrogen (N) and phosphorus (P) deficiencies are important wheat yield limiting factors. There is an information gap regarding differences between single element and binary N-P sources. The objective of this paper was to develop a general model for N-P fertilization in the area that also quantified diammonium phosphate (DAP) effect and its interactions with N-urea, applied at sowing or tillering, as compared with triple superphosphate (TSP). Between 1984 and 1985, 13 experiments were carried out in farmer’s fields N. With the yield data for each experiment, a yield function was fitted which… More >

  • Open Access


    Nitrate reductase activity, biomass, yield, and quality in cotton in response to nitrogen fertilization

    Hernández-Cruz AE1, E Sánchez2*, P Preciado-Rangel1, M L García-Bañuelos2, A Palomo-Gil1, A Espinoza-Banda1

    Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 454-460, 2015, DOI:10.32604/phyton.2015.84.454

    Abstract In the production of cotton (Gossypium hirsutum L.), nitrogen fertilization is one of the most costly crop practices, but important to reach high yields. However, high nitrogen (N) content in plants does not always translate into a high fibre production. One way of assessing the efficiency of the N fertilizer is through the enzymatic activity of the nitrate reductase (NR). This is a key enzyme in N assimilation, whose activity is regulated by a number of endogenous and exogenous factors that determine yield. The aim of this study was to assess the effect of N fertilization on yield, fibre quality,… More >

  • Open Access


    Onion yield as affected by plant density, nitrogen level and loss of leaf area

    Siliquini OA1, GA Orioli2, JC Lobartini2

    Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 338-344, 2015, DOI:10.32604/phyton.2015.84.338

    Abstract Onion (Allium cepa) is an important horticulture crop because of its value as a food with a long shelf life being a relatively non-perishable product. It is very helpful to understand the growth response of the seeded onion crop to conduct appropriate field practices in attaining the highest or optimum yields. A three year field experiment was conducted using a variety of onion Valcatorce INTA, in a randomized block design with five replicates. Treatments were two plant densities and three rates of N application. The bulb growth followed a classical sigmoid curve. During the rapid growing period, the crop had… More >

  • Open Access


    Fertilization effects with P on accumulated leaf area duration, biomass and yield of three cultivars of maize in Toluca, Mexico

    Díaz López E1, A Morales Ruíz1, EJ Vargas Ramírez1, P Hernández Herrera1, RC Reséndiz Melgar1, I Brena Hernández2, JM Loeza Corte2

    Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 319-330, 2015, DOI:10.32604/phyton.2015.84.319

    Abstract The effect of six phosphorus levels (0, 40, 80, 120, 160 and 200 kg/ha) on the duration of cumulative leaf area, biomass and agronomic yield was determined in the maize cultivars: Amarillo Almoloya, Cacahuacintle and Condor in 2010 and 2011. Such cultivars were sown in the Cerrillo Piedras Blancas Mexico. A completely randomized complete block design with factorial arrangement was utilized. High phosphorus levels (120, 160 and 200 kg/ha) positively affected the duration of cumulative leaf area; greatest values were obtained in Cacahuacintle. A greater duration of accumulated leaf area contributes to determine high values of biomass accumulation and grain… More >

  • Open Access


    Impact of selenium fertilization on the activity of detoxifying enzymes of H2O2 in bean plants

    Hermosillo-Cereceres MA1, E Sánchez1, E Muñoz-Márquez1, A Guevara-Aguilar1, M García-Bañuelos1, D Ojeda-Barrios2

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 347-352, 2014, DOI:10.32604/phyton.2014.83.347

    Abstract Selenium (Se) is an essential element for many organisms, although in high concentrations it may become toxic, leading to the generation of reactive oxygen species (ROS). In this study, bean plants received different application rates of Se (0, 10, 20, 40, 80, and 160 µM) in the form of selenite and selenate to evaluate the activity of the detoxifying enzymes of H2O2. The results indicated that the activity of these enzymes in bean plants depended on the chemical form of Se: selenite at rates of 20 µM or greater diminished biomass and yield, increasing the activity of superoxide dismutase (SOD).… More >

  • Open Access


    Cotton production with high sowing densities using organic fertilization

    López Martínez JD1, E Salazar Sosa1, HI Trejo-Escareño1, JL García Hernández1, M Navarro Morones2, C Vázquez-Vázquez1

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 237-242, 2014, DOI:10.32604/phyton.2014.83.237

    Abstract The Laguna Region located in the states of Durango and Coahuila, Mexico, is the main cow milk production region of Mexico. Here there are about 500000 heads of cattle. This region produces about 1.2 million tons of cattle manure annually, which allows raising the possibility of their use in agriculture. Treatments evaluated in this study were: plant density (120000 and 240000 plants/ha), solarized cattle manure (0, 40, 80, 120 t/ha), and an additional chemical treatment of 120-60-00 kg/ha of nitrogen, phosphorus and potassium, respectively. A randomized block design with a strip arrangement was used. Three replicates were used per treatment.… More >

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