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  • Open Access

    ARTICLE

    Morphometric and Biochemical Changes in Agave americana L. Plantlets Induced By Ethyl Methanesulfonate

    S. J. Reyes-Zambrano1,†, M. L. Ramírez-Merchant1,†, C. Arias-Castro2, M. A. Rodríguez-Mendiola2, C. A. Lecona-Guzmán1, V. M. Ruíz-Valdiviezo1, D. González-Mendoza3, F. A. Gutiérrez-Miceli1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.3, pp. 277-284, 2019, DOI:10.32604/phyton.2019.06504

    Abstract A. americana L. is a crop with very little genetic variability. In order to evaluate the effect of ethyl methanesulfonate (EMS) to induce variability in in vitro plantlets of A. americana, different explants (meristems, leaves and roots) were evaluated for the production of callus. MS medium supplemented with ANA (2.68 μM) and BAP (2.68 μM) was used. Callus obtained from apical meristem were treated with 15 mM EMS for two hours after which shoot formation was induced using 2,4-D (0.11 μM) and BAP (44 μM). The EMS induced variations in the morphometric and morphological parameters of the plantlets obtained, with… More >

  • Open Access

    ARTICLE

    Total Phenols, Flavonoids and Antioxidant Activity in Annona muricata and Annona purpurea Callus Culture

    M. Y. Ovando-Domínguez1, M. C. Luján-Hidalgo1, D. González-Mendoza2, A. A. Vargas-Díaz3, N. Ruiz-Lau1,4, F. A. Gutiérrez-Miceli1, C. A. Lecona-Guzmán1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 139-147, 2019, DOI:10.32604/phyton.2019.06546

    Abstract Callus cultures of Annona muricata and Annona purpurea were induced in Murashige and Skoog (MS) medium supplemented with different concentrations of 1-naphthylacetic acid (NAA), 6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) utilized hypocotyls with explant. The highest percentage of callus formation was the treatment supplemented with 3 mg L-1 NAA for A. muricata (100%) while for A. purpurea in lower percentage (75%). BA stimulated the formation of shoots in all the evaluated concentrations, being the concentration of 2 mg L-1 the one that induced the greater formation of shoots for A. muricata (23 shoots/explant) and A. purpurea (28 shoots/explant). The content… More >

  • Open Access

    ARTICLE

    Isocyanate-Free Polyurethanes by Coreaction of Condensed Tannins with Aminated Tannins

    M. Thébault1,2, A. Pizzi13*, F.J. Santiago-Medina1, F.M. Al-Marzouki3, S. Abdalla3

    Journal of Renewable Materials, Vol.5, No.1, pp. 21-29, 2017, DOI:10.7569/JRM.2016.634116

    Abstract Isocyanate-free polyurethane resins biosourced to a very high percentage level were prepared by the reaction of aminated mimosa tannin extract with commercial mimosa tannin extract prereacted with dimethyl carbonate. The reaction took place with ease at ambient temperature. Indications were that the polyurethanes obtained formed a hard film when cured at a temperature higher than 100 °C. Furthermore, the carbohydrate fraction of the tannin extract also appeared to be carbonated and reacted to generate isocyanate-free polyurethane linkages with the aminated tannins. This indicated that not only the polyphenolic fraction of the tannin extract, but also its other major component, can… More >

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