Zoran S. Ilić^1,*, Ljiljana Stanojević², Lidija Milenković¹, Ljubomir Šunić¹, Dragana Lalević¹, Aleksandra Milenković², Žarko Kevrešan³

Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2515-2527, 2025, DOI:10.32604/phyton.2025.069082 - 29 August 2025

Abstract This study aims to determine the phytochemical composition and antioxidant activity (AA) of different plant parts (bulbs, stalk, leaves and flowers) of wild rosy garlic (Allium roseum) from Montenegro. The flower exhibited the highest concentration of total phenols (55.7 GAE/g d.e.), followed by the leaf (25.6 mg GAE/g d.e.). The leaf displayed the highest concentration of total flavonoids (41.48 mg RE/g d.e.), followed by the flower (36.26 mg RE/g d.e.) and top part of the stalk (26.80 mg RE/g d.e.). The AA of different parts of A. roseum after 60 min of incubation decreased in the following… More >

Menghan Li^1,2,#, Yuhui He^1,#, Changning Chen², Li Liu², Jia Xu², Jiahao Cao^1,2, Xiaotong Guo^1,*, Linlin Dong^1,2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2575-2591, 2025, DOI:10.32604/phyton.2025.064518 - 29 August 2025

Abstract Codonopsis pilosula is a major Qi-tonifying medicinal herb, and its active composition is analyzed systematically. However, the relationship between its production origins and commodity specification grades with the active composition of C. pilosula lacks systematic research. This study integrates the HPLC and UV-Vis methodologies to evaluate the quality of C. pilosula from commodity specification grades and different origins, and it explores the correlation between ecological factors and production origins with active components. Here, network pharmacology is used to determine that lobetyolin, syringin, and tangshenoside I have potential efficacy in treating pulmonary fibrosis and oxidative stress. The HPLC and… More >

Lesya Voytenko^1,*, Mykola Shcherbatiuk¹, Valentyna Vasyuk¹, Kateryna Romanenko¹, Lidiya Babenko¹, Oleksandr Smirnov^2,3, Iryna Kosakivska¹

Phyton-International Journal of Experimental Botany, Vol.94, No.7, pp. 2195-2214, 2025, DOI:10.32604/phyton.2025.067772 - 31 July 2025

Abstract Prolonged lack of rain and high-temperature lead to soil water deficits, inhibiting cereal crop growth in early ontogenesis and reducing grain quality and yield. Rye (Secale cereale L.) is a key grain crop, particularly in regions where wheat cultivation is challenging or unfeasible. To clarify its drought adaptation mechanisms, we analyzed the effects of moderate soil drought on growth, hormonal homeostasis, and the dynamics and distribution of free amino acids and phenolic compounds in rye at early vegetative stages and post-recovery. Drought triggered both general and organ-specific changes in endogenous phytohormones. A nonspecific response involved the… More >

Bismillah Mubeen^1,2,#, Ammarah Hasnain^2,3,#,*, Syed Atif Hasan Naqvi⁴, Fahad Hakim⁵, Syed Sheharyar Hassan Naqvi⁶, Muhammad Zeeshan Hassan⁴, Muhammad Umer Iqbal⁷, Mahmoud Moustafa⁸, Mohammed O. Alshaharni⁸, Mingzheng Duan^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.7, pp. 1941-1971, 2025, DOI:10.32604/phyton.2025.064056 - 31 July 2025

Abstract Plants have long served as a cornerstone for drug discovery, offering a vast repertoire of bioactive compounds with proven efficacy in combating oxidative stress, a pivotal driver of chronic diseases such as cancer, diabetes, cardiovascular disorders, and neurodegenerative conditions. This review synthesizes current knowledge on plant-derived antioxidants, emphasizing their mechanisms, therapeutic potential, and quantitative efficacy validated through standardized assays. Key phytochemicals, including polyphenols, carotenoids, flavonoids, and terpenoids, neutralize reactive oxygen species (ROS) through radical scavenging, enzyme modulation, and gene regulation. For instance, lutein, a carotenoid found in leafy greens, demonstrates potent antioxidant activity with IC₅₀ values… More >

Claude Y. Hamany Djande, Paul A. Steenkamp, Ian A. Dubery^*

Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1269-1286, 2025, DOI:10.32604/phyton.2025.062392 - 30 April 2025

Abstract Centella asiatica L., a medicinal herb, has attracted substantial interest in research as well as commercial domains due to its bioactive compounds which include the pentacyclic triterpenoid centellosides, and in addition, hydroxy. In addition, hydroxycinnamic acid conjugates as well as flavonoids. The latter is the major class of secondary plant metabolites and comprises various subclasses, including anthocyanidins. Anthocyanins are rarely reported in extracts from C. asiatica and differ structurally due to a flavylium (2-phenylchromenylium) ion that carries a positive charge at the oxygen atom of the C-ring of the basic flavonoid structure. Callus of C. asiatica was initiated… More >

Mehmet Fidan¹, Behcet Inal^2,*, Mesut Sırrı³, Bülent Hallaç⁴, Ulutas Mehmet Sefa¹

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 781-791, 2025, DOI:10.32604/phyton.2025.062171 - 31 March 2025

Abstract The use of conventional herbal medicines is a rapidly expanding phenomenon in developed nations. For instance, 30%–50% of all drug use in China is attributed to traditional herbal preparations. Current study evaluated the antioxidant (DPPH, FRAP), total phenolic and flavonoid content, antibacterial activity, and DNA damage protective potential of aqueous and methanolic extracts obtained from the aerial parts and roots of Cymbocarpum wiedemannii Boiss., an endemic plant in Turkey. In antioxidant analyses, the methanolic extract of the aerial parts showed the highest %DPPH (73.38) and IC50 (3.46 mg/mL) values. The FRAP analysis revealed the highest iron-reducing… More > Graphic Abstract

Xinxin Chen¹, Fuxin Li¹, Jingyu Jia¹, Yajing Tian¹, Xin Li^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 825-842, 2025, DOI:10.32604/phyton.2025.062015 - 31 March 2025

Abstract In plants, transcription factor (TF) family LATERAL ORGAN BOUNDARIES DOMAIN (LBD) has been identified to be involved in the ripening and senescence processes of fruits. However, the function of LBD in Hylocereus undatus (Haw.) Britton & Rose (H. undatus) has not been reported yet. Through transcriptomic analysis, virus-induced gene silencing (VIGS) technology, and RT-qPCR validation, we investigated the role of the LBD TFs in the senescence of H. undatus. Transcriptomic analysis revealed that HuLBD1 is a key transcription factor of the LBD family regulating H. undatus senescence. After silencing HuLBD1, 5075 differentially expressed genes (DEGs) were identified. GSEA results showed… More >

Enyan Chen^1,#, Jingyu Jia^1,3,#, Jiaju Sun¹, Jie Wang², Xinxin Chen¹, Xin Li^1,3,4,*

Phyton-International Journal of Experimental Botany, Vol.93, No.11, pp. 2837-2856, 2024, DOI:10.32604/phyton.2024.057932 - 30 November 2024

Abstract During post-harvest storage of Cucumis sativus fruit, the application of trypsin treatment could increase flavonoid compound levels and reduce oxidative damage. To investigate the mechanism of trypsin-induced flavonoid biosynthesis in C. sativus, we conducted a combined analysis of transcriptomics and widely targeted metabolomics. One hundred and seventy-five significantly different metabolites were obtained from metabolomics data. The kyoto encyclopedia of genes and genomes (KEGG) functional enrichment results indicated that these metabolites were mainly involved in the phenylpropanoid biosynthesis pathway. By combining the results of the weighted gene co-expression network analysis (WGCNA) with the 130 upregulated phenylpropanoid metabolites, 22… More >

Xiaodong Shi^*, Tingyu Wang, Sui Ai, Jiasi Li

Phyton-International Journal of Experimental Botany, Vol.93, No.10, pp. 2571-2581, 2024, DOI:10.32604/phyton.2024.056606 - 30 October 2024

Abstract Cotton rose (Hibiscus mutabilis) is a well-known ornamental plant that produces large flowers of vibrant colors. However, metabolites in H. mutabilis flowers with vibrant color have not been fully understood. By performing a combined analysis of metabolomics and transcriptomics data, we here explored mechanisms for the production of primary active compounds in this plant. Multivariate statistics unveiled differences in flavonoid metabolism between white and pink flowers, with pink flowers exhibiting a greater flavonoid abundance. The white-to-pink transition of cotton rose flowers may be attributed to pelargonidin-3-O-glucoside formation. On examining the expression of genes related to the structure More > Graphic Abstract

Lili Liu¹, Can Hu⁴, Huilin Cheng¹, Chuangju Ma¹, Jiancheng Sun¹, Yunyi Chen², Manxi Wu², Han Yang², Cui Sun^2,3, Jinping Cao^2,3, Dengliang Wang^1,*, Yue Wang^2,*, Chongde Sun^2,3

Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2499-2518, 2024, DOI:10.32604/phyton.2024.054449 - 30 September 2024

Abstract Oral ulcers, a common mucosal affliction, severely diminish patients’ quality of life due to pain and discomfort. Despite existing treatments, the frequent recurrence of oral ulcers underscores the need for more effective therapeutic options. This study aimed to investigate the inhibitory effects of Ponkan (Citrus reticulata ‘Ponkan’) extracts and their flavonoid components on oral ulcers, exploring their potential as a natural therapy. Twenty different Ponkan cultivars were selected for flavonoid extraction and analysis. Ultra-performance liquid chromatography (UPLC) and high-resolution mass spectrometry (HRMS) were utilized for substance identification, leading to the discovery of 16 flavonoid compounds. Using… More > Graphic Abstract