Fayaz Muhammad¹, Jahangir Khan¹, Asad Ullah¹, Fasee Ullah¹, Razaullah Khan², Inayat Khan², Mohammed ElAffendi³, Gauhar Ali^3,*

CMC-Computers, Materials & Continua, Vol.77, No.1, pp. 647-664, 2023, DOI:10.32604/cmc.2023.038748

Abstract IIF (Indirect Immune Florescence) has gained much attention recently due to its importance in medical sciences. The primary purpose of this work is to highlight a step-by-step methodology for detecting autoimmune diseases. The use of IIF for detecting autoimmune diseases is widespread in different medical areas. Nearly 80 different types of autoimmune diseases have existed in various body parts. The IIF has been used for image classification in both ways, manually and by using the Computer-Aided Detection (CAD) system. The data scientists conducted various research works using an automatic CAD system with low accuracy. The diseases in the human body… More >

Jun Xiang^1,2,*, Xiaoqing Chen¹, Qi Liu¹, Huihua Jing², Tongqiang Chen², Wanli Tang², Wenyang Xu²

Journal of Renewable Materials, Vol.11, No.10, pp. 3739-3750, 2023, DOI:10.32604/jrm.2023.028090

Abstract Biomass-derived carbon dots (C-dots) are considered a very important carbon material in metal ion detection of their small environmental impact, simple preparation process, and relatively low cost. A green approach for synthesizing biomass-derived C-dots from Chenpi using a hydrothermal method without further processing is proposed in the present study. The as-synthesized C-dots show excellent fluorescence properties, superior resistance to UV irradiation photobleaching, and high photostability in salt-containing solutions. The C-dots were used in the form of label-free fluorescent probes for sensitively detecting Hg²⁺ selectively. The outcome relationship behaved linearly and was established based on a given range between 10–300 nM… More > Graphic Abstract

Ruizhi Huang¹, Huasheng Yu², Yong Yang¹, Heqin Liu², Xuelong Wu¹, Zhihong Liu¹, Haiyan He¹, Gengwei Wu¹, Wengjia Wang¹, Hua Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 333-348, 2023, DOI:10.32604/phyton.2022.023252

Abstract As global temperature rise, the threat of heat stress to rapeseed production is becoming more obvious. Exploring the response characteristics of two important biological pathways, oil accumulation and photosynthesis, to heat stress during B. napus seed filling is helpful in the genetic improvement of heat-tolerant rapeseed. The effects of heat stress on seed oil accumulation and chlorophyll fluorescence characteristics of 29 B. napus germplasms with different oil content and environmental sensitivity, including 6 rapeseed varieties which exhibited environment-sensitive/insensitive and with high, medium or low oil content, were tested by whole plant heat stress or the in vitro silique culture system.… More >

Shuang Song¹, Xiaomei Li², Xin Wang¹, Qi Zhou¹, Yongping Li¹, Xiaojing Wang¹, Shoukun Dong^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.11, pp. 2475-2490, 2022, DOI:10.32604/phyton.2022.021090

Abstract Soybean is an important legume food crop, and its seeds are rich in nutrients, providing humans and animals with edible oil and protein feed. However, soybean is sensitive to water requirements, and drought is an important factor limiting soybean yield and quality. This study used Heinong 84 (drought resistant variety) and Hefeng 46 (intermediate variety) as tested varieties planted in chernozem, albic, and black soils. The effects of drought stress on the activities of key enzymes in carbon metabolism and photosynthetic characteristics of soybean were studied during the flowering stage, most sensitive to water. (1) The activities of SS-1, 6PGDH,… More >

MEIGE WANG, JUAN GONG, CHUNHUI SONG, ZHENGYANG WANG, SHANGWEI SONG, JIAN JIAO, MIAOMIAO WANG, XIANBO ZHANG^*, TUANHUI BAI^*

BIOCELL, Vol.46, No.7, pp. 1763-1770, 2022, DOI:10.32604/biocell.2022.018934

Abstract

Drought stress is one of the major environmental obstacles that limit the production and development of apples (Malus domestica Borkh.). The role of melatonin is well known in the protection of plants under environmental stresses. In this study, we investigated the effect of melatonin on apple rootstock M. hupehensis Rehd under drought stress. The results showed that drought inhibited the growth of M. hupehensis and dramatically reduced root surface area, root volume, the number of tips and forks, and root diameter. Drought-induced growth inhibition was significantly decreased by adding melatonin. Net photosynthetic rate (Pn), intercellular CO₂ concentration (Ci), stomatal conductance… More >

Ekaterina Yotsova¹, Martin Stefanov¹, Georgi Rashkov¹, Margarita Kouzmanova², Anelia Dobrikova¹, Emilia Apostolova^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1365-1380, 2022, DOI:10.32604/phyton.2022.020566

Abstract The aim of this study was to assess the impact of the microalgae Chlorella vulgaris on the rice seedlings at physiological conditions and under cadmium (Cd) stress. We examined the effects of C. vulgaris in the nutrient solution on rice seedlings grown hydroponically in the presence and the absence of 150 μM CdCl₂, using the low (77 K) temperature and pulse amplitude modulated (PAM) chlorophyll fluorescence, P700 photooxidation measurements, photochemical activities of both photosystems, kinetic parameters of oxygen evolution, oxidative stress markers (MDA, H₂O₂ and proline), pigment content, growth parameters and Cd accumulation. Data revealed that the application C. vulgaris not… More >

Ekhlaque A. Khan^1,2,*, Hamdino M. I. Ahmed³, Meena Misra¹, Pallavi Sharma^1,4, Amarendra N. Misra¹

Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 959-973, 2022, DOI:10.32604/phyton.2022.018708

Abstract Cadmium (Cd), a life threatening hazardous heavy metal is abundant in nature. Cd amounts are greater in leaves than other plant parts, and it shows considerable effects on photosynthesis. Nitric oxide (NO), a free radical present in living organisms, is now known as an important signaling molecule playing various physiological processes in plants. In this study, the possible ameliorative effect of NO on photosynthesis was examined on pea seedlings grown under Cd stress. Results showed that chlorophyll, net photosynthetic rate, transpiration rate, stomatal conductance, photochemical efficiency of Photosystem II and Photosystem I decreased, and Fo and non-photochemical parameters for PSII… More >

CHEN CAI¹, HAO SUN², LIANG HU³, ZHICHAO FAN^1,*

BIOCELL, Vol.45, No.2, pp. 229-257, 2021, DOI:10.32604/biocell.2021.014338

Abstract Integrin molecules are transmembrane αβ heterodimers involved in cell adhesion, trafficking, and signaling. Upon activation, integrins undergo dynamic conformational changes that regulate their affinity to ligands. The physiological functions and activation mechanisms of integrins have been heavily discussed in previous studies and reviews, but the fluorescence imaging techniques –which are powerful tools for biological studies– have not. Here we review the fluorescence labeling methods, imaging techniques, as well as Förster resonance energy transfer assays used to study integrin expression, localization, activation, and functions. More >

Hui Yao¹, Liqun Wang³, Jia Guo¹, Weimin Liu⁴, Jingjing Li¹, Yingxiao Wang², Linhong Deng^1,*, Mingxing Ouyang^1,2,3,*

Molecular & Cellular Biomechanics, Vol.17, No.3, pp. 101-111, 2020, DOI:10.32604/mcb.2020.09595

Abstract Prostate cancer is the most common cancer among men beyond 50 years old, and ranked the second in mortality. The level of Prostate-specific antigen (PSA) in serum has been a routine biomarker for clinical assessment of the cancer development, which is detected mostly by antibody-based immunoassays. The proteolytic activity of PSA also has important functions. Here a genetically encoded biosensor based on fluorescence resonance energy transfer (FRET) technology was developed to measure PSA activity. In vitro assay showed that the biosensor containing a substrate peptide ‘RLSSYYSGAG’ had 400% FRET change in response to 1 µg/ml PSA within 90 min, and… More >

Yanwei Feng¹, Wen Lei², Rui Gu³, Ping Zhao⁴, Shijun Ni^1,*, Ningfei Lei^3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 667-679, 2020, DOI:10.32604/phyton.2020.010160

Abstract Water, minerals, nutrients, etc., can be shared by physiological integration among inter-connected ramets of clonal plants. Nitrogen plays an important role in alleviating cadmium (Cd) stress for clonal plants. But how different forms of nitrogen affect growth performance of clonal plants subjected to heterogeneous Cd stress still remains poorly understood. A pot experiment was conducted to investigate the differential effects of ammonium and nitrate on growth performance of Glechoma longituba under heterogeneous Cd stress. In the experiment, parent ramets of Glechoma longituba clonal fragments were respectively supplied with modified Hoagland solution containing 7.5 mM ammonium, 7.5 mM nitrate or the… More >