XUHUI ZHAO^1,2, XIAOMIN HUANG¹, CHUNYAN DANG², XIA WANG¹, YUJIAO QI³, HONGLING LI^2,*

Oncology Research, Vol.32, No.5, pp. 999-1009, 2024, DOI:10.32604/or.2024.046679

Abstract Background: EBV-miR-BARTs exhibit significant relevance in epithelial tumors, particularly in EBV-associated gastric and nasopharyngeal cancers. However, their specific mechanisms in the initiation and progression of gastric cancer remain insufficiently explored. Material and Methods: Initially, EBV-miRNA-BART6-5p and its target gene SMAD4 expression were assessed in EBV-associated gastric cancer tissues and cell lines. Subsequent transfection induced overexpression of EBV-miRNA-BART6-5p in AGS and MKN-45, and downregulation in EBV-positive cells (SUN-719). The subsequent evaluation aimed to observe their impact on gastric cancer cell proliferation, migration, and glycolytic processes, with the TGF-β/SMAD4 signaling pathway value clarified using a TGF-β inhibitor. Results: EBV-miRNA-BART6-5p exhibits pronounced upregulation… More >

JINGLONG CAO^1,#, SHUMEI LI^2,#, TONG ZHANG^1,#, JIAN LIU¹, WENSHUANG HOU¹, ANQI WANG¹, CHANG WANG^3,4,*, CHENGHAO JIN^1,3,5,*

BIOCELL, Vol.48, No.2, pp. 313-325, 2024, DOI:10.32604/biocell.2023.043474

Abstract Background: Valtrate (Val) was extracted from the Valeriana jatamansi Jones plant, had good antitumor activity. However, its precise molecular mechanism in cancer cells was still unclear. This study investigated the effect of Val on gastric cancer (GC) cells and its potential molecular mechanism. Methods: Cell viability was examined by CCK-8 assay. Cell cycle, apoptosis, and Reactive oxygen species (ROS) level were analyzed by flow cytometry. The migration effect of Val on AGS cells was analyzed by transwell and wound-healing assay. The expression levels of proteins were analyzed by western blot. Results: The cell viability assay results demonstrated that Val significantly… More > Graphic Abstract

WEIXUE WANG^#, TONGTONG WANG^#, YAN ZHANG, TING DENG, HAIYANG ZHANG^*, YI BA^*

Oncology Research, Vol.32, No.3, pp. 489-502, 2024, DOI:10.32604/or.2023.046676

Abstract Different from necrosis, apoptosis, autophagy and other forms of cell death, ferroptosis is a mechanism that catalyzes lipid peroxidation of polyunsaturated fatty acids under the action of iron divalent or lipoxygenase, leading to cell death. Apatinib is currently used in the third-line standard treatment of advanced gastric cancer, targeting the anti-angiogenesis pathway. However, Apatinib-mediated ferroptosis in vascular endothelial cells has not been reported yet. Tumor-secreted exosomes can be taken up into target cells to regulate tumor development, but the mechanism related to vascular endothelial cell ferroptosis has not yet been discovered. Here, we show that exosomes secreted by gastric cancer… More >

XIANGDONG SUN^1,2,#, HUIJUAN WEN^1,2,#, FAZHAN LI^1,2, IHTISHAM BUKHARI^1,2, FEIFEI REN^1,2, XIA XUE^1,2, PENGYUAN ZHENG^1,2,*, YANG MI^1,2,*

Oncology Research, Vol.32, No.2, pp. 283-296, 2024, DOI:10.32604/or.2023.044618

Abstract Nicotinamide adenine dinucleotide (NAD+) plays an essential role in cellular metabolism, mitochondrial homeostasis, inflammation, and senescence. However, the role of NAD+-regulated genes, including coding and long non-coding genes in cancer development is poorly understood. We constructed a prediction model based on the expression level of NAD+ metabolism-related genes (NMRGs). Furthermore, we validated the expression of NMRGs in gastric cancer (GC) tissues and cell lines; additionally, β-nicotinamide mononucleotide (NMN), a precursor of NAD+, was used to treat the GC cell lines to analyze its effects on the expression level of NMRGs lncRNAs and cellular proliferation, cell cycle, apoptosis, and senescence-associated secretory… More > Graphic Abstract

TINGTING LI¹, WEI ZHONG¹, LIU YANG¹, ZHIYU ZHAO¹, LI WANG¹, CONG LIU¹, WANYUN LI¹, HAIYAN LV², SHENGYU WANG¹, JIANGHUA YAN¹, TING WU^1,*, GANG SONG^1,*, FANGHONG LUO^1,*

Oncology Research, Vol.32, No.2, pp. 361-371, 2024, DOI:10.32604/or.2023.043807

Abstract The high mortality rate associated with gastric cancer (GC) has resulted in an urgent need to identify novel therapeutic targets for GC. This study aimed to investigate whether GAIP interacting protein, C terminus 1 (GIPC1) represents a therapeutic target and its regulating mechanism in GC. GIPC1 expression was elevated in GC tissues, liver metastasis tissues, and lymph node metastases. GIPC1 knockdown or GIPC1 blocking peptide blocked the platelet-derived growth factor receptor (PDGFR)/PI3K/AKT signaling pathway, and inhibited the proliferation and migration of GC cells. Conversely, GIPC1 overexpression markedly activated the PDGFR/PI3K/AKT signaling pathway, and promoted GC cell proliferation and migration. Furthermore,… More > Graphic Abstract

MESFER AL SHAHRANI, REEM GAHTANI, MOHAMMAD ABOHASSAN, MOHAMMAD ALSHAHRANI, YASSER ALRAEY, AYED DERA, MOHAMMAD RAJEH ASIRI, PRASANNA RAJAGOPALAN^*

Oncology Research, Vol.32, No.2, pp. 251-259, 2024, DOI:10.32604/or.2023.043139

Abstract Gastric cancers are caused primarily due to the activation and amplification of the EGFR or HER2 kinases resulting in cell proliferation, adhesion, angiogenesis, and metastasis. Conventional therapies are ineffective due to the intra-tumoral heterogeneity and concomitant genetic mutations. Hence, dual inhibition strategies are recommended to increase potency and reduce cytotoxicity. In this study, we have conducted computational high-throughput screening of the ChemBridge library followed by in vitro assays and identified novel selective inhibitors that have a dual impediment of EGFR/HER2 kinase activities. Diversity-based High-throughput Virtual Screening (D-HTVS) was used to screen the whole ChemBridge small molecular library against EGFR and… More > Graphic Abstract

JIAZHUANG LI^*, WEI ZHANG, SHOUBAO GAO, LI SUN, QINGYANG TAI, YING LIU

BIOCELL, Vol.47, No.10, pp. 2313-2320, 2023, DOI:10.32604/biocell.2023.029860

Abstract Background: Trastuzumab resistance accounts for chemotherapy failure in gastric cancer patients in clinical practice. The significance of long non-coding RNAs (lncRNAs) in the maintenance of drug resistance in gastric cancer has been already underlined. Method: This study aimed to identify the specific role of lncRNA-ATB in gastric cancer progression and trastuzumab resistance. The downstream miRs of lncRNA-ATB and target genes of miRs were predicted by bioinformatics analysis and verified using dual luciferase reporter assay. Loss- and gain-function assays were performed to explore the roles of lncRNA-ATB, miR-200c, and zinc-finger protein 217 (ZNF217) in the cell functions and trastuzumab resistance of… More >

GAOZHONG LI, FUXIN LI, NING WEI, QING JIA^*

BIOCELL, Vol.47, No.9, pp. 2081-2099, 2023, DOI:10.32604/biocell.2023.029986

Abstract Background: This study was aimed at identifying natural killer (NK) cell-related genes to design a risk prognosis model for the accurate evaluation of gastric cancer (GC) prognosis. Methods: We obtained NK cell-related genes from various databases, followed by Cox regression analysis and molecular typing to identify prognostic genes. Various immune algorithms and enrichment analyses were used to investigate the mutations, immune status, and pathway variations among different genotypes. The key prognostic genes were assessed using the least absolute shrinkage and selection operator (Lasso) regression analysis and univariate Cox regression analysis. Thereafter, the risk score (RS) prognosis model was constructed based… More > Graphic Abstract

JUNYU HE^1,2,3, FENG ZENG^1,2,3, XI JIN^1,2,3, LIN LIANG^1,2,3, MENGXIANG GAO^1,2,3, WENTAO LI^1,2,3, GUIYUAN LI^1,2,3, YANHONG ZHOU^1,2,3,*

Oncology Research, Vol.31, No.4, pp. 615-630, 2023, DOI:10.32604/or.2023.029698

Abstract Fos-related antigen 1 (Fra-1) is a nuclear transcription factor that regulates cell growth, differentiation, and apoptosis. It is involved in the proliferation, invasion, apoptosis and epithelial mesenchymal transformation of malignant tumor cells. Fra-1 is highly expressed in gastric cancer (GC), affects the cycle distribution and apoptosis of GC cells, and participates in GC occurrence and development. However, the detailed mechanism of Fra-1 in GC is unclear, such as the identification of Fra-1-interacting proteins and their role in GC pathogenesis. In this study, we identified tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein eta (YWHAH) as a Fra-1-interacting protein in GC cells using co-immunoprecipitation… More >

MIN WANG^1,#, HAILIANG ZHAO^1,2,#, WEIWEI CHEN^3,#, CAIQUN BIE^4,#, JINYING YANG¹, WENRUI CAI¹, CHUTIAN WU¹, YANFANG CHEN¹, SHUFEN FENG¹, YING SHI¹, YUTING LI¹, HUIJUN TANG⁴, LIXIAN ZHONG¹, LILIANGZI GUO¹, SISI CHEN¹, LINJING LONG⁵, SHAOHUI TANG^1,*

Oncology Research, Vol.31, No.4, pp. 515-541, 2023, DOI:10.32604/or.2023.029101

Abstract The dysregulation of exosomal microRNAs (miRNAs) plays a crucial role in the development and progression of cancer. This study investigated the role of a newly identified serum exosomal miRNA miR-4256 in gastric cancer (GC) and the underlying mechanisms. The differentially expressed miRNAs were firstly identified in serum exosomes of GC patients and healthy individuals using next-generation sequencing and bioinformatics. Next, the expression of serum exosomal miR-4256 was analyzed in GC cells and GC tissues, and the role of miR-4256 in GC was investigated by in vitro and in vivo experiments. Then, the effect of miR-4256 on its downstream target genes… More > Graphic Abstract