RONGXUE WEI¹, CHUNCHUN HAN^1,*, FENGJIANG YE¹, SHOUHAI WEI², FANG HE¹, HEHE LIU¹, LIANG LI¹, HONGYONG XU¹, SHENQIANG HU¹, XIANYIN ZENG²

BIOCELL, Vol.46, No.1, pp. 171-183, 2022, DOI:10.32604/biocell.2022.015409

Abstract In order to explore the role of forkhead box protein O1 (FoxO1) in the lipid metabolism and cell proliferation, goose primary hepatocytes were isolated and incubated with insulin or PI3K-Akt-mTOR pathway dual inhibitor NVP-BEZ235, and then transfected with FoxO1 interference plasmid. The related parameters of lipid metabolism and cell proliferation were measured. The results firstly showed that FoxO1 interference increased the intracellular TG and lipids concentration (P < 0.05); and increased the proliferative index (PI), cell DNA synthesis, protein expression of Cyclin D1 in goose primary hepatocytes (P < 0.05). Secondly, the co-treatment of insulin and FoxO1 interference increased the… More >

Salome Kiparoidze^1,*, Nino Karumidze², Ekaterine Bakuradze¹, Irina Modebadze¹, Levan Rusishvili¹, Dimitri Kordzaia², Diana Dzidziguri¹

Molecular & Cellular Biomechanics, Vol.18, No.3, pp. 131-138, 2021, DOI:10.32604/mcb.2021.015596

Abstract According to the literature, different mechanisms and kinetics proceeding of regenerative growth has been established using the basic models of liver regeneration (after resection or chemically induced). Hence, in order to determine general regularities of the adaptive-compensatory processes in various pathological conditions, the processes taking place in the cholestatic liver of adult white rats during the first four days after common bile duct ligation have been studied. It has been shown that in cholestatic liver, compensatory-adaptive processes take place with different kinetics compared to those after resection. In particular, in response to the increased functional load caused by destructive processes… More >

Dongdong Zheng^1,#, Chi Shen^1,2,#, Wenshi Liu¹, Wenjing Lv¹, Xiaofei Li^1,*

Congenital Heart Disease, Vol.16, No.4, pp. 373-381, 2021, DOI:10.32604/CHD.2021.015260

Abstract Objectives: Pulmonary arterial hypertension in congenital heart disease (PAH-CHD) is the most common type of PAH and increases morbidity and mortality in patients with CHD. Right heart catheterization (RHC) is the standard method to diagnose PAH. However, RHC is an invasive and complicated method with relatively high cost. Noninvasive, feasible, and cost-efficient methods are urgently needed. The objective of this study was to evaluate three potential biomarkers of PAH-CHD: Hepatocyte growth factor (HGF), osteopontin (OPN), and suppression of tumorigenicity 2 (ST2). Methods: Plasma samples were collected from patients with CHD (n = 46) and healthy individuals (n = 22) and… More >

Cristiane Dos Santos VERGILIO, Edésio José Tenório De MELO^*

BIOCELL, Vol.37, No.2, pp. 45-54, 2013, DOI:10.32604/biocell.2013.37.045

Abstract Cadmium (Cd) induces several effects in different tissues, but our knowledge of the toxic effects on organelles is insufficient. To observe the progression of Cd effects on organelle structure and function, HuH-7 cells (human hepatic carcinoma cell line) were exposed to CdCl2 in increasing concentrations (1 μM – 20 μM) and exposure times (2 h – 24 h). During Cd treatment, the cells exhibited a progressive decrease in viability that was both time- and dose-dependent. Cd treated cells displayed progressive morphological changes that included cytoplasm retraction and nuclear condensation preceding a total loss of cell adhesion. Treatment with 10 μM… More >

Lidia M. Lopez*, Marta Dubin**, Patricia H. Carrizo**, Mario H. Burgos***, Amanda Pellegrino De Iraldi*, Andrés O. M. Stoppani**

BIOCELL, Vol.27, No.2, pp. 213-224, 2003, DOI:10.32604/biocell.2003.27.213

Abstract CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho[1,2b]pyran- 5,6-dione; CG-NQ), a β-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 µM CG-NQ, the following effects were observed: (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or peroxysomes; and (h) production of hydrogen… More >

Fan Feng¹, Tingting Xia¹, Runze Zhao¹, Mengyue Wang¹, Li Yang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 131-131, 2019, DOI:10.32604/mcb.2019.07211

Abstract Objective: To study the effects of different substrate stiffness on human hepatocytes using RNA sequencing (RNA-Seq) technology. The stiffness was corresponding to physiology and pathology stiffness of liver tissues.
Results: With the aid of RNA-Seq technology, our study characterizes the transcriptome of hepatocytes cultured on soft, moderate, stiff and plastic substrates. Compared to soft substrate, our RNA-Seq results revealed 1131 genes that were up-regulated and 2534 that were down-regulated on moderate substrate, 1370 genes that were up-regulated and 2677 down-regulated genes on stiff substrate. Functional enrichment analysis indicated that differentially expressed genes were associated with the regulation of actin… More >

Berengere PAPEGAY^{1, *}, Michaela STADLER¹, Vincent Nuyens¹, Isabelle SALMON², Veronique KRUYS³, Jean G. BOOGAERTS¹

BIOCELL, Vol.38, No.1, pp. 25-32, 2014, DOI:10.32604/biocell.2014.38.025

Abstract Ischemia-reperfusion injury is a determinant in liver injury occurring during surgery, ischemic states and multiple organ failure. The pre-existing nutritional status of the liver, i.e., fasting, might contribute to the extent of tissue injury. This study investigated whether alanine, an amino acid precursor of glucose, could protect ex vivo perfused livers of fasting rats from reperfusion injury. The portal vein was cannulated, the liver removed and perfused in a closed ex vivo system. Isolated livers were perfused either with glucose 1 g/L and 10 g/L, or with equal concentrations of alanine (n = 10 in each group). The experiment consisted… More >

Odin RAMÍREZ-FERNÁNDEZ^1,*, Rafael GODÍNEZ¹, Esmeralda ZUÑIGA-AGUILAR¹, Luis E. GÓMEZ-QUIROZ², María C. GUTIÉRREZ-RUIZ², Juan MORALES³, Roberto OLAYO³

BIOCELL, Vol.39, No.2-3, pp. 9-14, 2015, DOI:10.32604/biocell.2015.39.009

Abstract We have designed and evaluated a radial-flow bioreactor for three-dimensional liver carcinoma cell culture on a new porous coated scaffold. We designed a culture chamber where a radial flow of culture medium was continuously delivered through it. Once this system was established, flow was simulated using flow dynamics software based on numeric methods to solve Navier-Stockes flow equations. Perfusion within cell culture scaffolds was simulated using a flow velocity of 7 mL/min and found that cell culture medium was distributed unhindered in the bioreactor chamber. Afterwards, the bioreactor was built according to the simulated design and was tested with liver… More >

Elizabeth PÉREZ-TEJADA^1,4*, Juan MORALES-CORONA³, Luis Ernesto GÓMEZ-QUIRÓZ², María Concepción GUTIERREZ-RUIZ², Roberto OLAYO³

BIOCELL, Vol.41, No.2-3, pp. 41-44, 2017, DOI:10.32604/biocell.2017.41.041

Abstract Low pressure plasma polymer films were synthesized using pyrrole and allylamine monomers and adding iodine was used (or not) for the reaction in both cases. They were polymerized on glass substrates under the same reaction conditions. Polymerization of allylamine was also studied at different operating powers. These thin polymer films were used as culture surfaces for HepG2 cells, a cell line derived from a human hepatoma. The proliferation, differentiation and two-dimensional propagation until obtaining monolayer of the cells was studied on the different synthetized films and correlations were established between the conditions of synthesis, the physicochemical characteristics obtained and the… More >