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Search Results (9)
  • Open Access


    Forkhead box protein O1 (FoxO1) regulates lipids metabolism and cell proliferation mediated by insulin and PI3K-Akt-mTOR pathway in goose primary hepatocytes


    BIOCELL, Vol.46, No.1, pp. 171-183, 2022, DOI:10.32604/biocell.2022.015409

    Abstract In order to explore the role of forkhead box protein O1 (FoxO1) in the lipid metabolism and cell proliferation, goose primary hepatocytes were isolated and incubated with insulin or PI3K-Akt-mTOR pathway dual inhibitor NVP-BEZ235, and then transfected with FoxO1 interference plasmid. The related parameters of lipid metabolism and cell proliferation were measured. The results firstly showed that FoxO1 interference increased the intracellular TG and lipids concentration (P < 0.05); and increased the proliferative index (PI), cell DNA synthesis, protein expression of Cyclin D1 in goose primary hepatocytes (P < 0.05). Secondly, the co-treatment of insulin and FoxO1 interference increased the… More >

  • Open Access


    Study of Hepatocytes Polyploidization Peculiarities in Cholestatic Liver of Adult Rats

    Salome Kiparoidze1,*, Nino Karumidze2, Ekaterine Bakuradze1, Irina Modebadze1, Levan Rusishvili1, Dimitri Kordzaia2, Diana Dzidziguri1

    Molecular & Cellular Biomechanics, Vol.18, No.3, pp. 131-138, 2021, DOI:10.32604/mcb.2021.015596

    Abstract According to the literature, different mechanisms and kinetics proceeding of regenerative growth has been established using the basic models of liver regeneration (after resection or chemically induced). Hence, in order to determine general regularities of the adaptive-compensatory processes in various pathological conditions, the processes taking place in the cholestatic liver of adult white rats during the first four days after common bile duct ligation have been studied. It has been shown that in cholestatic liver, compensatory-adaptive processes take place with different kinetics compared to those after resection. In particular, in response to the increased functional load caused by destructive processes… More >

  • Open Access


    Plasma HGF and OPN as Potential Biomarkers of Pulmonary Arterial Hypertension in Congenital Heart Disease

    Dongdong Zheng1,#, Chi Shen1,2,#, Wenshi Liu1, Wenjing Lv1, Xiaofei Li1,*

    Congenital Heart Disease, Vol.16, No.4, pp. 373-381, 2021, DOI:10.32604/CHD.2021.015260

    Abstract Objectives: Pulmonary arterial hypertension in congenital heart disease (PAH-CHD) is the most common type of PAH and increases morbidity and mortality in patients with CHD. Right heart catheterization (RHC) is the standard method to diagnose PAH. However, RHC is an invasive and complicated method with relatively high cost. Noninvasive, feasible, and cost-efficient methods are urgently needed. The objective of this study was to evaluate three potential biomarkers of PAH-CHD: Hepatocyte growth factor (HGF), osteopontin (OPN), and suppression of tumorigenicity 2 (ST2). Methods: Plasma samples were collected from patients with CHD (n = 46) and healthy individuals (n = 22) and… More >

  • Open Access


    Autophagy, apoptosis and organelle features during cell exposure to cadmiumč

    Cristiane Dos Santos VERGILIO, Edésio José Tenório De MELO*

    BIOCELL, Vol.37, No.2, pp. 45-54, 2013, DOI:10.32604/biocell.2013.37.045

    Abstract Cadmium (Cd) induces several effects in different tissues, but our knowledge of the toxic effects on organelles is insufficient. To observe the progression of Cd effects on organelle structure and function, HuH-7 cells (human hepatic carcinoma cell line) were exposed to CdCl2 in increasing concentrations (1 μM – 20 μM) and exposure times (2 h – 24 h). During Cd treatment, the cells exhibited a progressive decrease in viability that was both time- and dose-dependent. Cd treated cells displayed progressive morphological changes that included cytoplasm retraction and nuclear condensation preceding a total loss of cell adhesion. Treatment with 10 μM… More >

  • Open Access


    Apoptogenic effect of the lipophilic o-naphthoquinone CG 10-248 on rat hepatocytes: light and electron microscopy studies

    Lidia M. Lopez*, Marta Dubin**, Patricia H. Carrizo**, Mario H. Burgos***, Amanda Pellegrino De Iraldi*, Andrés O. M. Stoppani**

    BIOCELL, Vol.27, No.2, pp. 213-224, 2003, DOI:10.32604/biocell.2003.27.213

    Abstract CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho[1,2b]pyran- 5,6-dione; CG-NQ), a β-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 µM CG-NQ, the following effects were observed: (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or peroxysomes; and (h) production of hydrogen… More >

  • Open Access


    Gene Expression Profiling of Human Hepatocytes Grown on Differing Substrate Stiffness

    Fan Feng1, Tingting Xia1, Runze Zhao1, Mengyue Wang1, Li Yang1,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 131-131, 2019, DOI:10.32604/mcb.2019.07211

    Abstract Objective: To study the effects of different substrate stiffness on human hepatocytes using RNA sequencing (RNA-Seq) technology. The stiffness was corresponding to physiology and pathology stiffness of liver tissues.
    Results: With the aid of RNA-Seq technology, our study characterizes the transcriptome of hepatocytes cultured on soft, moderate, stiff and plastic substrates. Compared to soft substrate, our RNA-Seq results revealed 1131 genes that were up-regulated and 2534 that were down-regulated on moderate substrate, 1370 genes that were up-regulated and 2677 down-regulated genes on stiff substrate. Functional enrichment analysis indicated that differentially expressed genes were associated with the regulation of actin… More >

  • Open Access


    Alanine minimises hepatocyte injury after ischemia-reperfusion in an ex vivo rat liver model

    Berengere PAPEGAY1, *, Michaela STADLER1, Vincent Nuyens1, Isabelle SALMON2, Veronique KRUYS3, Jean G. BOOGAERTS1

    BIOCELL, Vol.38, No.1, pp. 25-32, 2014, DOI:10.32604/biocell.2014.38.025

    Abstract Ischemia-reperfusion injury is a determinant in liver injury occurring during surgery, ischemic states and multiple organ failure. The pre-existing nutritional status of the liver, i.e., fasting, might contribute to the extent of tissue injury. This study investigated whether alanine, an amino acid precursor of glucose, could protect ex vivo perfused livers of fasting rats from reperfusion injury. The portal vein was cannulated, the liver removed and perfused in a closed ex vivo system. Isolated livers were perfused either with glucose 1 g/L and 10 g/L, or with equal concentrations of alanine (n = 10 in each group). The experiment consisted… More >

  • Open Access


    Hepatocyte culture in a radial-flow bioreactor with plasma polypyrrole coated scaffolds


    BIOCELL, Vol.39, No.2-3, pp. 9-14, 2015, DOI:10.32604/biocell.2015.39.009

    Abstract We have designed and evaluated a radial-flow bioreactor for three-dimensional liver carcinoma cell culture on a new porous coated scaffold. We designed a culture chamber where a radial flow of culture medium was continuously delivered through it. Once this system was established, flow was simulated using flow dynamics software based on numeric methods to solve Navier-Stockes flow equations. Perfusion within cell culture scaffolds was simulated using a flow velocity of 7 mL/min and found that cell culture medium was distributed unhindered in the bioreactor chamber. Afterwards, the bioreactor was built according to the simulated design and was tested with liver… More >

  • Open Access


    Effect of synthesis variables of plasma synthesized polymers on growth of HepG2 cells

    Elizabeth PÉREZ-TEJADA1,4*, Juan MORALES-CORONA3, Luis Ernesto GÓMEZ-QUIRÓZ2, María Concepción GUTIERREZ-RUIZ2, Roberto OLAYO3

    BIOCELL, Vol.41, No.2-3, pp. 41-44, 2017, DOI:10.32604/biocell.2017.41.041

    Abstract Low pressure plasma polymer films were synthesized using pyrrole and allylamine monomers and adding iodine was used (or not) for the reaction in both cases. They were polymerized on glass substrates under the same reaction conditions. Polymerization of allylamine was also studied at different operating powers. These thin polymer films were used as culture surfaces for HepG2 cells, a cell line derived from a human hepatoma. The proliferation, differentiation and two-dimensional propagation until obtaining monolayer of the cells was studied on the different synthetized films and correlations were established between the conditions of synthesis, the physicochemical characteristics obtained and the… More >

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