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  • Open Access

    ARTICLE

    Isolation and molecular identification of cellulolytic bacteria from Dig Rostam hot spring and study of their cellulase activity

    Sareh HAJIABADI1, Mansour MASHREGHI1, Ahmad Reza BAHRAMI1,2, Kiarash GHAZVINI3, Maryam M. MATIN1,2,*

    BIOCELL, Vol.44, No.1, pp. 63-71, 2020, DOI:10.32604/biocell.2020.08171

    Abstract Cellulose is the main structural component of lignocellulosic wastes that can be converted to sugars and biofuels by cellulase. Due to wide applications of this enzyme in various industries around the world, cellulase is considered as the third industrial enzyme. The ability of thermophilic bacteria in the production of heat-stable cellulases has made them valuable tools in biotechnology. The aim of this study was isolation and molecular identification of cellulolytic thermophile bacteria from Dig Rostam hot spring and investigating their cellulase activity. Samples were taken from water and sediments of this hot spring, and cellulolytic bacteria were enriched in media… More >

  • Open Access

    ARTICLE

    Identification, population dynamics and parasitism of aphids in pecan in the Comarca Lagunera, Mexico

    Ávila-Rodríguez V1, CA Vacio-Fraga1, U Nava-Camberos2, C Márquez-Hernández1, C García de la Peña1, V Olalde-Portugal3, JL García-Hernández2

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 249-255, 2016, DOI:10.32604/phyton.2016.85.249

    Abstract Pecan Carya illinoinensis (Wang.) K. Koch is one of the most important crops in northern Mexico. In the Comarca Lagunera, during 2013, the pecan acre age was 7075 ha, a nut production of 8648 ton, and a value of US$ 29.9 million (US$ 4230/ha). The aphids Monellia caryella Fitch., Monelliopsis pecanis Bissell and Melanocallis caryaefoliae Davis (Hemiptera: Aphididae) are among the most important insect pests associated with the pecan. The objectives of this study were to identify the species of aphids, determine their population dynamics and parasitism, in pecan orchards with different insect pest management programs, in the Comarca Lagunera.… More >

  • Open Access

    ARTICLE

    First morphogenetic identification of Fusarium solani isolated from orange fruit in Egypt

    Abd-Elsalam KA1,2, K Youssef1,2, H Almoammar3

    Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 128-131, 2015, DOI:10.32604/phyton.2015.84.128

    Abstract Losses due to postharvest decay may occur at any time during postharvest handling, from harvest to consumption affecting the produce quality and quantity. Accurate identification of the pathogen causing postharvest disease is essential to the selection of an appropriate disease control approach. Nine isolates of Fusarium recovered from orange fruit were identified as Fusarium solani. The fungus is involved with fruit decay. The obtained cultures were purified and grown on potato-dextrose agar (PDA), malt yeast agar (MYA), and Czapek's nutrient media (CNM) under light for identification. A pathogenicity test was carried out to fulfil Koch's postulates. The pathogen could only… More >

  • Open Access

    ARTICLE

    Molecular markers to study the variability within the Eragrostis curvula complex

    Zappacosta D1, M Meier1, A Carrera1, G Pacheco2, S Cardone3, JP Selva1, V Echenique1

    Phyton-International Journal of Experimental Botany, Vol.80, pp. 211-220, 2011, DOI:10.32604/phyton.2011.80.211

    Abstract Weeping lovegrass is well adapted for forage production and useful for soil conservation in semiarid regions, constituting a morphologically diverse group. Diploid genotypes are unfrequent and reproduce sexually, whereas the tetraploids and plants of higher ploidy levels reproduce by apomixis. In the present work RAPD, AFLP and EST-SSR were used in order to assess the reproductive mode through progeny tests, to determine intracultivar homogeneity or seed purity, to establish genetic relationships among the cultivars within the complex and to characterize the new materials obtained by our group. Eight commercial cultivars and three new plant materials were analyzed. Uniform and variable… More >

  • Open Access

    ARTICLE

    Identification of axillary buds of potato seedlings based on a vision system with fuzzy logic

    Martínez Corral L1, E Martínez-Rubin2, F F lores-García3, M Vázquez-Rueda3, J Frías-Ramírez2, MA Segura-Castruita2

    Phyton-International Journal of Experimental Botany, Vol.80, pp. 79-84, 2011, DOI:10.32604/phyton.2011.80.079

    Abstract Potato (Solanum tuberosum L.) is a crop whose production yield at national level is very low compared with that in the most productive countries. This is because it is a partially automated crop with deficient and inadequate agronomic practices, low technification levels and great quantity of work wages required per hectare of cultivation. The necessity to generate technical and modern procedures that increase crop production, quality and yield has fostered development of projects leading to obtain seedlings free of pathogens with material of high genetic, physiological and sanitary quality. Utilization of a vision system for the computerized visual recognition of… More >

  • Open Access

    ARTICLE

    Seed and seedling morphology of Dicliptera squarrosa Nees (Acanthaceae) as a character identification source, and its relationship with survival structures

    Perissé P1, RJ Lovey2, CV Arias3, M Scandaliaris2, ML Molinelli1

    Phyton-International Journal of Experimental Botany, Vol.80, pp. 73-78, 2011, DOI:10.32604/phyton.2011.80.073

    Abstract Dicliptera squarrosa Nees, “canario rojo”, “coral de campo” or “ajicillo”, is a perennial native herb of the north, center and east of Argentina. It is cited as an ornamental, apiarian, emergency forage plant, and as a glyphosate-tolerant weed. The objectives were to characterize the seed and seedling of D. squarrosa and to establish the relationships with the structures that ensure its survival. The materials were treated with the conventional methodology to perform morphological studies, using stereoscopically and optical microscopy. The germination assays were carried out to determine the germination percentage and the germination type. The weight of 1000 seeds was… More >

  • Open Access

    ARTICLE

    Quantitative measures of leaf epidermal cells as a taxonomic and phylogenetic tool for the identification of Stanhopea species (Orchidaceae)

    Foroughbakhch R, RJ Ferry Sr, JL Hernández-Piñero, MA Alvarado-Vázquez, A Rocha-Estrada

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 113-127, 2008, DOI:10.32604/phyton.2008.77.113

    Abstract Orchids of the genus Stanhopea are currently identified only by their floral structure characteristics. A statistical analysis of a significant number of species of this genus disclosed that measurements of adaxial and abaxial epidermal cell surface areas can be correlated with specific recognized species by a leaf printing method. This allows an objectively either positive or nearly positive confirmation of the identity of a species in the absence of flowers and without damage to plants. When ordering the mean values obtained for these surface areas in each species in a decreasing order, a correlation was observed in a hierarchical way… More >

  • Open Access

    ARTICLE

    Morphological and genetic characteristics allow the identification of a collection of garlic cultivars in the North-Central region of Mexico

    Azuara Hernández1 L, H Silos Espino1, C Perales Segovia1, JF Gómez Leyva2, AG Alpuche Solís3, LM Macias Valdez4

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 81-91, 2008, DOI:10.32604/phyton.2008.77.081

    Abstract The objective of this study was to compare the yield and genetic relationships between two Perla garlic selections, obtained by individual selection of cloves, and commercial varieties and cultivars adapted to diverse regions of Mexico (which did not have a selection process). Varieties with fewer cloves showed higher yields. Coreano and California produced 20 ton/ha (fresh weight) and Perla selections between 17-19 ton/ha (fresh weight). Six primers of the series OPB were tested for genetic characterization and OPB-17 was selected. With the amplified DNA fragments, a bina-ry matrix was generated; afterwards, a dissimilarity matrix and dendrogram were developed. High genetic… More >

  • Open Access

    ARTICLE

    Identification and evolutionary relationships of partial gene sequences from dehydrin group in three species of cacti

    Hernández-Camacho S1, E Pérez-Molphe-Balch1, AG Alpuche-Solís2, JF Morales-Domínguez1

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 151-162, 2017, DOI:10.32604/phyton.2017.86.151

    Abstract Dehydrins or Group 2 Late Embryogenesis Abundant (LEA) proteins play an important role in the response and adaptation to different types of abiotic stresses such as droughts, high salinity and low temperatures. Using PCR techniques, we identified three gene fragments that encoded dehydrin-like proteins in three cactispecies Opuntia ficus-indica (OpfiDHN-like), Leuchtenbergia principis (LepDHN-like) and Mammillaria bombycina (MabDHN-like). Bioinformatic sequence analysis showed an identity between 96 and 97% with the Opuntia streptacantha dehydrin 1 (OpsDHN1) gene, demonstrating that the amplified fragments corresponded to dehydrin-like gene sequences, and that the designed oligonucleotides were effective for similar gene amplification in different cacti genera.… More >

  • Open Access

    REVIEW

    A Review of Structural Health Monitoring Techniques as Applied to Composite Structures

    Amafabia, Daerefa-a Mitsheal1, Montalvão, Diogo2, David-West, Opukuro1, Haritos, George1

    Structural Durability & Health Monitoring, Vol.11, No.2, pp. 91-147, 2017, DOI:10.3970/sdhm.2017.011.091

    Abstract Structural Health Monitoring (SHM) is the process of collecting, interpreting and analysing data from structures in order to determine its health status and the remaining life span. Composite materials have been extensively use in recent years in several industries with the aim at reducing the total weight of structures while improving their mechanical properties. However, composite materials are prone to develop damage when subjected to low to medium impacts (i.e. 1-10 m/s and 11-30 m/s respectively). Hence, the need to use SHM techniques to detect damage at the incipient initiation in composite materials is of high importance. Despite the availability… More >

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