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  • Open Access

    ARTICLE

    Effect of Putrescine on Low-Temperature Acclimation in Chlamydomonas reinhardtii

    Muslum S. Inal1,2, Dilek Unal1,*, Bengu Turkyilmaz Unal3,*, Munir Ozturk4

    Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 583-598, 2022, DOI:10.32604/phyton.2022.018223

    Abstract Putrescine is reported to be necessary for cold acclimation under low-temperature stress. In this study, the effect of low-temperature on some physiological and biochemical parameters has been investigated using the green algae Chlamydomonas reinhardtii. The lipid peroxidation rate, amount of Rubisco protein, activities of antioxidant enzymes and gene expression of polyamine biosynthesis (odc2, and spd1), heat shock proteins (hsp70c, hsp90a, and hsp90c), and PSII repair mechanisms (psba, rep27, and tba1) were determined to understand the low-temperature response. Exogenous putrescine application significantly increased Rubisco protein concentration and catalase enzyme activities under low-temperature stress. Moreover, real-time RT-PCR results and gene expression analysis… More >

  • Open Access

    ARTICLE

    High-Throughput MicroRNA and mRNA Sequencing Reveals that MicroRNAs May Be Involved in Pectinesterase-Mediated Cold Resistance in Potato

    Chongchong Yan1, Qianqian Wang1, Nan Zhang2, Jiajia Wang1, Xuexiang Ren1, Bingjie Xue3, Xiaojing Pu4, Zhihong Xu4, Huajun Liao1,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 561-586, 2020, DOI:10.32604/phyton.2020.010322

    Abstract Since potato cultivars are sensitive to low temperature, cold injury severely affects the geographical distribution and yield of potato. Although some miRNAs have been identified in response to cold stress in plants, there is no report about the role of miRNAs in the response to cold stress in potato. Here, via high throughput sequencing, we described the profiling of cold stress response to miRNA and mRNA in potato. Two small RNA and six mRNA libraries were constructed and sequenced. 296 known and 211 novel miRNAs were identified, in which 34 miRNAs in Cold Group (CG) had the higher expression quantity… More >

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