Home / Advanced Search

  • Title/Keywords

  • Author/Affliations

  • Journal

  • Article Type

  • Start Year

  • End Year

Update SearchingClear
  • Articles
  • Online
Search Results (51)
  • Open Access


    Identification and Evaluation of Insect and Disease Resistance in Transgenic Cry1Ab13-1 and NPR1 Maize

    Yongjing Xi, Zhou Yang, Yukun Jin, Jing Qu, Shuyan Guan, Siyan Liu, Piwu Wang*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1257-1274, 2023, DOI:10.32604/phyton.2023.025918

    Abstract PCR detection, quantitative real-time PCR (q-RTPCR), outdoor insect resistance, and disease resistance identification were carried out for the detection of genetic stability and disease resistance through generations (T2, T3, and T4) in transgenic maize germplasms (S3002 and 349) containing the bivalent genes (insect resistance gene Cry1Ab13-1 and disease resistance gene NPR1) and their corresponding wild type. Results indicated that the target genes Cry1Ab13-1 and NPR1 were successfully transferred into both germplasms through tested generations; q-PCR confirmed the expression of Cry1Ab13-1 and NPR1 genes in roots, stems, and leaves of tested maize plants. In addition, S3002 and 349 bivalent gene-transformed lines… More >

  • Open Access


    Identification and Characterization of ZF-HD Genes in Response to Abscisic Acid and Abiotic Stresses in Maize

    Xiaojie Jing1,2,3,#, Chunyan Li1,2,3,#, Chengjuan Luo1,2,3, Chaonan Yao1,2,3, Jiahao Zhang1,2,3, Tingting Zhu1,2,3, Jiuguang Wang1,2,3, Chaoxian Liu1,2,3,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 707-723, 2023, DOI:10.32604/phyton.2023.024338

    Abstract The zinc finger homeodomain (ZF-HD) genes belong to the homeobox gene family, playing critical roles in flower development and stress response. Despite their importance, however, to date there has been no genome-wide identification and characterization of the ZF-HD genes that are probably involved in stress responses in maize. In this study, 24 ZF-HD genes were identified, and their chromosomal locations, protein properties, duplication patterns, structures, conserved motifs and expression patterns were investigated. The results revealed that the ZF-HD genes are unevenly distributed on nine chromosomes and that most of these genes lack introns. Six and two ZF-HD genes have undergone… More >

  • Open Access


    Distribution, Etiology, Molecular Genetics and Management Perspectives of Northern Corn Leaf Blight of Maize (Zea mays L.)

    M. Ashraf Ahangar1, Shabir Hussain Wani1,*, Zahoor A. Dar2, Jan Roohi1, Fayaz Mohiddin1, Monika Bansal3, Mukesh Choudhary4, Sumit K. Aggarwal4, S. A. Waza1, Khursheed Ahmad Dar5, Ayman El Sabagh6,7, Celaleddin Barutcular8, Omer Konuşkan9, Mohammad Anwar Hossain10,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2111-2133, 2022, DOI:10.32604/phyton.2022.020721

    Abstract Maize is cultivated extensively throughout the world and has the highest production among cereals. However, Northern corn leaf blight (NCLB) disease caused by Exherohilum turcicum, is the most devastating limiting factor of maize production. The disease causes immense losses to corn yield if it develops prior or during the tasseling and silking stages of crop development. It has a worldwide distribution and its development is favoured by cool to moderate temperatures with high relative humidity. The prevalence of the disease has increased in recent years and new races of the pathogen have been reported worldwide. The fungus E. turcicum is… More >

  • Open Access


    Effects of Auxin at Different Concentrations on the Growth, Root Morphology and Cadmium Uptake of Maize (Zea mays L.)

    Lingyan Hu, Haiyan Chen, Guangqun Zhang, Zihao Yu, Kai Yan, Fangdong Zhan, Yongmei He*

    Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1933-1944, 2022, DOI:10.32604/phyton.2022.020041

    Abstract Indoleacetic acid (IAA) is an important regulator that plays a crucial role in plant growth and responses to abiotic stresses. In the present study, a sand cultivation experiment was carried out to investigate the effects of IAA at different concentrations (0, 0.01, 0.1, 0.5, 1, and 2.5 mmol/L) on maize growth, root morphology, mineral elements (Ca, Mg) and Cd uptake under 20 mg/kg Cd stress. The results showed that 0.01 mmol/L is the optimal IAA concentration for enhancing the Cd tolerance of maize. Compared with the control treatment, 0.01 mmol/L IAA promoted maize growth, with significant increases in the height,… More >

  • Open Access


    Optimization of Callus Induction Conditions from Immature Embryos of Maize under Stress

    Xiaotong Wei1,#, Xuhong Fan2,#, Xuan Lu1, Liyuan Han1, Honglin Zhang3, Siyan Liu3, Jing Qu3, Shuyan Guan3,*, Yiyong Ma3,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1945-1957, 2022, DOI:10.32604/phyton.2022.020062


    The embryos of maize (Zea mays L.) inbred lines GS02, GS07, GS08, GS11 and GS15 were used as receptor materials to optimize the receptor system from the aspects of genotype, medium components and stress (PEG6000, mannitol, salt and low phosphorus). The results showed that GS07 had the highest induction rate (95.2%). Orthogonal test analysis showed that the best combination of medium components in induction was A2B3C1D3(2), namely, the concentration of 2, 4-dichlorophenoxy acetic acid (2,4-D) was 4 mg·mL-1, the concentration of L-Proline (L-Pro) was 0.8 mg·mL-1, and the concentration of silver nitrate (AgNO3) was 10 mg·mL-1 (or 5 mg·mL). Interestingly,… More >

  • Open Access


    Development of a New Cold-Tolerant Maize (Zea mays L.) Germplasm Using the ICE1 Gene from Arabidopsis thaliana

    Jing Qu1, Shuang Liu2, Peng Jiao2, Zhenzhong Jiang2, Jianbo Fei2, Shuyan Guan1,*, Yiyong Ma1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1709-1719, 2022, DOI:10.32604/phyton.2022.018854

    Abstract To develop cold-tolerant maize germplasms and identify the activation of INDUCER OF CRT/DRE-BINDING FACTOR EXPRESSION (ICE1) expression in response to cold stress, RT-PCR was used to amplify the complete open reading frame sequence of the ICE1 gene and construct the plant expression vector pCAMBIA3301-ICE1-Bar. Immature maize embryos and calli were transformed with the recombinant vector using Agrobacterium tumefaciens-mediated transformations. From the regenerated plantlets, three T1 lines were screened and identified by PCR. A Southern blot analysis showed that a single copy of the ICE1 gene was integrated into the maize (Zea mays L.) genomes of the three T1 generations. Under… More >

  • Open Access


    Discovering Candidate Chromosomal Regions Linked to Kernel Size-Related Traits via QTL Mapping and Bulked Sample Analysis in Maize

    Hameed Gul1, Mengya Qian1, Mohammad G. Arabzai1,2, Tianhui Huang1, Qiannan Ma1, Fangyu Xing1, Wan Cao1, Tingting Liu1, Hong Duan1, Qianlin Xiao1,*, Zhizhai Liu1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1429-1443, 2022, DOI:10.32604/phyton.2022.019842

    Abstract Kernel size-related traits, including kernel length, kernel width, and kernel thickness, are critical components in determining yield and kernel quality in maize (Zea mays L.). Dissecting the phenotypic characteristics of these traits, and discovering the candidate chromosomal regions for these traits, are of potential importance for maize yield and quality improvement. In this study, a total of 139 F2:3 family lines derived from EHel and B73, a distinct line with extremely low ear height (EHel), was used for phenotyping and QTL mapping of three kernel size-related traits, including 10-kernel length (KL), 10-kernel width (KWid), and 10-kernel thickness (KT). The results… More >

  • Open Access


    Meta-Analysis of Flowering-Related Traits and Mining of Candidate Genes in Maize

    Jianyu Lu1, Qingping Jiang1, Tongyu Liu1, Yangyang Zhou1, Mengtong Liu1, Jing Qu2, Yiyong Ma2,*, Shuyan Guan2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1199-1209, 2022, DOI:10.32604/phyton.2022.019298

    Abstract Maize flowering is an important agronomic character, which is controlled by quantitative trait loci (QTL). Over the years, a large number of flowering-related QTL have been found in maize and exist in public databases. However, combining these data, re-analyzing and mining candidate loci and fine mapping of flowering-related traits to reduce confidence intervals has become a hot issue in maize. In this study, the QTL of 6 important agronomic traits of maize flowering were collected from 15 published articles, including flowering period (DA), Days to tasseling (DTT), Days to silking (DS), Days to pollen shedding (DTP), anthesis-silking interval (ASI) and… More >

  • Open Access


    Embryo and Endosperm Phytochemicals from Polyembryonic Maize Kernels and Their Relationship with Seed Germination

    J. David García-Ortíz1, Rebeca González-Centeno1, María Alejandra Torres-Tapia2, J. A. Ascacio-Valdés1, José Espinoza-Velázquez2, Raúl Rodríguez-Herrera1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 929-941, 2022, DOI:10.32604/phyton.2022.018368

    Abstract Because of the growing worldwide demand for maize grain, new alternatives have been sought for breeding of this cereal, e.g., development of polyembryonic varieties, which agronomic performance could positively impact the grain yield per unit area, and nutritional quality. The objectives of this study were to (1) determine the phytochemicals present in the embryo and endosperm of grain from maize families with high, low, and null polyembryony frequency, which were planted at different locations, and (2) state the relationship between these compounds and seed germination. The extracted phytochemicals from corn were identified by HPLC-MS. The results showed that the genotype… More >

  • Open Access


    Foliar Application of Cytokinin Modulates Gas Exchange Features, Water Relation and Biochemical Responses to Improve Growth Performance of Maize under Drought Stress

    M. Rafiqul Islam1,*, M. Shahinur Islam1, Nurunnaher Akter1, Mohammed Mohi-Ud-Din2, Mohammad Golam Mostofa3,4,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 633-649, 2022, DOI:10.32604/phyton.2022.018074

    Abstract Improvement of plant performance under drought stress is crucial to sustaining agricultural productivity. The current study investigated the ameliorative effects of foliar-applied kinetin, an adenine-type cytokinin (CK), on growth and gas exchange parameters, water relations and biochemical attributes of maize plants under drought stress. Eighteen-day-old maize plants were subjected to drought by maintaining soil moisture content at 25% field capacity for 8 days followed by foliar application of kinetin at 0, 75, 150 and 225 mg L−1 (CK0, CK75, CK150 and CK225, respectively) to the plants for two-times at the 9-day interval. Results revealed that drought stress markedly reduced stem diameter,… More >

Displaying 1-10 on page 1 of 51. Per Page