Guiling Xu^1,#, Xiaoxuan You^1,#, Yuehua Feng^1,2,*, Xiaoke Wang¹, Yuqi Gao¹, Hongjun Ren¹, Zhili Han¹, Jiale Li¹

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3251-3268, 2025, DOI:10.32604/phyton.2025.067997 - 29 October 2025

Abstract Nitrogen (N) reduction combined with organic fertilizer has become a highly popular fertilization method, meeting the sustainable development of agriculture. A field experiment was conducted to investigate the effects of N reduction (NR) and combined application of organic fertilizer (OF) on N utilization, yield, and quality of hybrid indica rice in the karst area. Using rice ‘Yixiangyou2115’ as the material, a split-plot design experiment was carried out with OF application rate as the main plots and NR rate as the subplots. The OF application rate had three levels: M0 (0 kg/ha), M1 (low OF, 1673… More >

Anton Louise P. De Ocampo^*

Revue Internationale de Géomatique, Vol.34, pp. 731-749, 2025, DOI:10.32604/rig.2025.066185 - 10 October 2025

Abstract The effects of climate change are becoming more evident nowadays, and the environmental stress imposed on crops has become more severe. Farmers around the globe continually seek ways to gain insights into crop health and provide mitigation as early as possible. Phenotyping is a non-destructive method for assessing crop responses to environmental stresses and can be performed using airborne systems. Unmanned Aerial Systems (UAS) have significantly contributed to high-throughput phenotyping and made the process rapid, efficient, and non-invasive for collecting large-scale agronomic data. Because of the high complexity and cost of specialized equipment used in… More >

Đorđe Vojnović¹, Robert Pokluda², Ivana Maksimović¹, Janko Červenski³, Sonja Ilin³, Boris Adamović¹, Jelena Popović-Đorđević^4,*

Phyton-International Journal of Experimental Botany, Vol.94, No.7, pp. 1997-2013, 2025, DOI:10.32604/phyton.2025.065535 - 31 July 2025

Abstract Onion (Allium cepa L.) is a widely cultivated vegetable valued for its taste, aroma, and nutritional content. Biostimulants (BTs) are substances that stimulate plant growth and development. This study explores the impact of BTs and nitrogen (N) fertilization on the yield and nutrient use efficiency of directly seeded onions, addressing the challenges of excessive N application and promoting sustainable practices. A two-year field experiment was conducted using a split-plot design with three replications. Treatments included a control (C), a seaweed extract-based BT (T1), humic and fulvic acids (T2), and Trichoderma spp. (T3). N rates applied were 64… More > Graphic Abstract

Shamim Ara Bagum¹, Mahbub Ul Islam², M Shalim Uddin^2,*, Sripati Sikder³, Ahmed Gaber⁴, Akbar Hossain^5,*

Phyton-International Journal of Experimental Botany, Vol.94, No.6, pp. 1897-1919, 2025, DOI:10.32604/phyton.2025.065607 - 27 June 2025

Abstract The yield of maize (Zea mays L.) is highly influenced by nitrogen fertilization. This study investigated the impact of nitrogen fertilization on morphophysiological traits in maize (Zea mays L.) and developed algorithms to relate manual phenotyping and digital phenotyping of maize with leaf nitrogen and digital field image traits. The experiment included three hybrid maize varieties, V1 (Hybrid 981), V2 (BARI Hybrid maize-9), and V3 (Hybrid P3396), which were evaluated across three nitrogen levels (N1 = 100 kg N ha⁻¹, N2 = 200 kg N ha⁻¹, N3 = 300 kg N ha⁻¹) in a split-plot design with three replications.… More >

Yuhe Wang^1,2, Haijun Sun³, Yaqiong Hao^2,4, Xiancan Zhu¹, Ju Min^2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.6, pp. 1735-1750, 2025, DOI:10.32604/phyton.2025.063868 - 27 June 2025

Abstract Over the past three decades, the expansion of intensive vegetable farming in southern China has led to excessive nitrogen (N) and phosphorus (P) fertilizer application, causing substantial N and P runoff losses. This study investigated four major vegetable production regions in southern China—the upper reaches of the Yangtze River (U-YR), the middle lower reaches of the Yangtze River (ML-YR), the Southeast Coast (SC), and the Pearl River basin (PR)—analyzing 175 published articles to characterize spatiotemporal patterns of N and P fertilizer applications and associated runoff losses from 1992 to 2021. The result showed that the… More >

Saif Ullah^1,2, Izhar Ali^1,2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1029-1045, 2025, DOI:10.32604/phyton.2025.064608 - 30 April 2025

Abstract Nitrogen (N) assimilation is crucial for the growth and development of C₃ plants, as it converts inorganic N into organic forms, important for protein synthesis, nucleic acids and other vital biomolecules. However, abiotic stressors such as drought, salinity, extreme temperatures and others significantly impact N uptake and utilization, thereby hindering plant growth and development. Recent advances in molecular biology have illuminated the complex networks that govern N assimilation under these stressful conditions, emphasizing the role of transcription factors, regulatory genes, and stress-responsive pathways. This review provides an integrated perspective on the latest research in nitrogen metabolism… More >

Kesu Wei¹, Guangju Liu², Bin Wei³, Qifang Zhang⁴, Shengjiang Wu¹, Zhaowei Li^2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1287-1298, 2025, DOI:10.32604/phyton.2025.062385 - 30 April 2025

Abstract To systematically examine the tolerance of flue-cured tobacco K326 to soil drought stress, this study set up different water content trials for K326 at 60%, 40%, and 20% levels during the vigorous growing period by greenhouse pot planting. Pigment content, chlorophyll fluorescence parameters, antioxidant enzyme activity, and SPS and GS activities associated with carbon and nitrogen metabolism were investigated in the K326 tobacco leaves. The results showed that chlorophyll a and chlorophyll b contents decreased, non-photochemical quenching coefficient Y(NPQ) decreased in the later stage of vigorous growth, and energy dissipation quantum yield Y(NO) levels increased… More >

Rui Zhang¹, Le Ren¹, Zhihang Liu¹, Xinyue Gu¹, Shanshan Du¹, Xiao Chen¹, Bingran Xi¹, Bingnan Zhao^1,2, Chao Si^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 709-721, 2025, DOI:10.32604/phyton.2025.062281 - 31 March 2025

Abstract Plant invasion poses a serious environmental challenge to freshwater ecosystems. Heavy metal pollution and nutrient enrichment, particularly nitrogen (N) and phosphorus (P), are two additional critical environmental issues known to influence the growth of invasive plants. Notably, the N/P ratio in the environments is often inconstant; however, whether these variations can cause differential responses in invasive plants remains underexplored, despite its crucial importance for understanding plant invasiveness. In this study, we examined the effects of different N/P ratios (4:1, 16:1, 32:1, 64:1) on the growth of a widespread invasive plant Alternanthera philoxeroides in cadmium (Cd) polluted… More >

Chang Liu^1,3, Chengcheng Yin¹, Jinjin Zhang², Haijun Sun^1,3,*

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 695-707, 2025, DOI:10.32604/phyton.2025.061790 - 31 March 2025

Abstract To promote the growth of cutting seeding of poplar (Populus L.), nitrogen (N) fertilizer and surface weed managements were required. We here conducted a pot experiment to examine the effects of natural vegetation, barnyardgrass (Echinochloa Beauv.), and sesbania (Sesbania cannabina pers.) on the growth of poplar cutting seedlings, soil properties, and ammonia (NH₃) volatilization under three N inputs (0, 0.5, and 1.5 g/pot, i.e., N0, N0.5, and N1, respectively). Results showed that N application promoted the growth of poplar cutting seedlings, including plant height, ground diameter, and biomass, compared with N0 treatment. Moreover, under N0, sesbania significantly increased… More >

Fei Li^1,2, Meili Ding¹, Hui Yuan¹, Siping Wang¹, Bin Liang^2,*

Phyton-International Journal of Experimental Botany, Vol.94, No.1, pp. 243-250, 2025, DOI:10.32604/phyton.2025.059499 - 24 January 2025

Abstract This study investigated the effects of chlorine-based fertilizers under varying nitrogen solution concentrations in a soilless culture system. The experiment included four nitrogen solution concentration levels, with nitrogen concentrations of 6 mmol/L (C1), 12 mmol/L (C2), 18 mmol/L (C3), and 24 mmol/L (C4). Each nutrient concentration level was further divided into four chloride ion treatments (R1, R2, R3, and R4), where 100%, 60%, 33%, and 0% of the NH₄⁺ and K⁺ ions were derived from NH₄Cl and KCl, respectively. The length, surface area and volume of root were significantly higher by 25.3%~136.9%, 40.1%~173.1%, 27.9%~178.0%, respectively, in the… More >