Bustingorri C, K Balestrasse, RS Lavado

Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 407-416, 2015, DOI:10.32604/phyton.2015.84.407

Abstract Arsenic (As) and Fluoride (F) are present in many soils, affecting crops and posing risks in the food chain. We performed pot experiments on spiked soils enriched in these elements either individually or simultaneously, over a wide range of concentrations. Soybean biomass production, grain yield, As and F accumulation and distribution within the plant, and the antioxidant response to these stresses were analyzed. Arsenic was more toxic than F. At As levels >35 mg/kg and F levels >375 mg/kg, yield loss reached 60% and 30%, respectively. At the highest dose of As plants died within 2 weeks, whereas F showed… More >

Galatro Andrea, Ivan Rousseau, Susana Puntarulo

Phyton-International Journal of Experimental Botany, Vol.75, pp. 7-20, 2006, DOI:10.32604/phyton.2006.75.007

Abstract Electron Paramagnetic Resonance (EPR) has been developed as a versatile field that uses different techniques sharing the common feature of resonant micro-wave radiation absorption by paramagnetic substances. Certain free radicals, such as ascorbyl radical (A•), show an stable spectrum, and can be directly detected by EPR at room temperature. Studies included in this work clearly show that this highly sensitive methodology can be successfully applied to biological systems. Exposure of plants to UV-B radiation was studied as a factor of oxidative stress in plants, and the effect on A^• steady state concentration was analyzed. A kinetic analysis was performed considering… More >

Berengere PAPEGAY^{1, *}, Michaela STADLER¹, Vincent Nuyens¹, Isabelle SALMON², Veronique KRUYS³, Jean G. BOOGAERTS¹

BIOCELL, Vol.38, No.1, pp. 25-32, 2014, DOI:10.32604/biocell.2014.38.025

Abstract Ischemia-reperfusion injury is a determinant in liver injury occurring during surgery, ischemic states and multiple organ failure. The pre-existing nutritional status of the liver, i.e., fasting, might contribute to the extent of tissue injury. This study investigated whether alanine, an amino acid precursor of glucose, could protect ex vivo perfused livers of fasting rats from reperfusion injury. The portal vein was cannulated, the liver removed and perfused in a closed ex vivo system. Isolated livers were perfused either with glucose 1 g/L and 10 g/L, or with equal concentrations of alanine (n = 10 in each group). The experiment consisted… More >

Natacha E PILONI, Elizabeth ROBELLO, Julián G BONETTO, Susana PUNTARULO^*

BIOCELL, Vol.40, No.1, pp. 39-42, 2016, DOI:10.32604/biocell.2016.40.039

Abstract Fe is essential for human life because it constitutes the required cofactor for proteins of diverse biological functions. However, the development of oxidative stress by exposure to excessive Fe, share signaling pathways with other treatments including activation of redox-sensitive factors. This study was focused on the comparison on the effects of Fe in the brain and other organs in vivo. The oxidative effects triggered by Fe overload strongly depend not only on the administration protocol, but also on the Fe-compound used, and the studied organ. In both the liver and the brain, Fe content drastically increased after Fe-dextran administration. However,… More >

Gabriela MALANGA, Paula Mariela GONZÁLEZ, Juan Manuel OSTERA, Susana PUNTARULO^*

BIOCELL, Vol.40, No.1, pp. 35-38, 2016, DOI:10.32604/biocell.2016.40.035

Abstract The harmful effects of the reactive species may be due to the increase in their steady state concentration either by the enhancement of their production rates and/or the decrease of their consumption rate by antioxidant activity. The ascorbyl radical (A^• ) can be considered as a final product of radical oxidative transformations of ascorbate (AH^-). The ratio A^• content/AH^- content (A^• /AH^-) has been widely used as an interesting tool to estimate mild to moderate oxidative transformations, providing a quick and simple method of diagnosis of stress in the hydrophilic cellular medium. The aim of this work was to summarize… More >

Marcelo HERNANDO¹, Christian HOUGHTON¹, Leda GIANNUZZI², Bernd KROCK³, Darío ANDRINOLO², Gabriela MALANGA^4,*

BIOCELL, Vol.40, No.1, pp. 23-26, 2016, DOI:10.32604/biocell.2016.40.023

Abstract Cyanobacteria are phototrophic organisms with great ecological and economical importance. Species of the genus Microcystis are known for their potential ability to synthesize toxins, notably microcystins. There is a growing interest in the evaluation of oxidative stress in relation to the impact of global climate change on natural ecosystems in different trophic levels. Several studies have focused on the analysis of organismal responses to mitigate the damage by controlling the generation of reactive oxygen species. Variations in environmental factors caused by climate change generate a situation of oxidative damage in Microcystis aeruginosa as a direct or indirect consequence. In this… More >

Nidia FERRAROTTI¹, Rosario MUSACCO-SEBIO², Christian SAPORITO-MAGRIÑÁ², Juan Manuel ACOSTA², Marisa REPETTO^{2 *}

BIOCELL, Vol.40, No.1, pp. 19-22, 2016, DOI:10.32604/biocell.2016.40.019

Abstract The objective of this work was to study the in vivo time course of biochemical processes of oxidative damage in the brain of Sprague-Dawley rats that received an acute overload of the redox active metals iron (Fe) and copper (Cu), and the redox inactive cobalt (Co) and nickel (Ni). Oxidative stress indicators (phospholipid and protein oxidation), glutathione (GSH), antioxidant enzymes and NADPH oxidase activities, and the plasma inflammatory cytokine (IL-6) were measured. The results showed that in brain oxidative mechanisms for both sets of metal are different, however in both cases are irreversible. The mechanism for Fe and Cu oxidative… More >

Jimena P. CABILLA, Sonia A. RONCHETTI, Beatriz H. DUVILANSKI^*

BIOCELL, Vol.40, No.1, pp. 15-18, 2016, DOI:10.32604/biocell.2016.40.015

Abstract Environmental contamination with some metalloids and heavy metals (M/HM) raises concern due to well known adverse effects on health. Among these pollutants, chromium VI (Cr VI), cadmium (Cd) and arsenic (As) are frequently present as a result of natural sources or due to industrial activities. They are able to easily enter the organism and negatively affect many organs and systems. In vivo (exposure to Cr VI, Cd or As through drinking water) and in vitro experiments (primary pituitary cell cultures) were performed in male Wistar rats to address their actions on hypothalamus-pituitary axis. All the M/HM accumulated in hypothalamus and… More >

WALTER MANUCHA

BIOCELL, Vol.31, No.1, pp. 1-12, 2007, DOI:10.32604/biocell.2007.31.001

Abstract Congenital obstructive nephropathy is the primary cause of end-stage renal disease in children. Rapid diagnosis and initiation of the treatment are vital to preserve function and/or to slow down renal injury. Obstructive uropathy effects -decline in the plasmatic renal flow and glomerular filtration rate, interstitial infiltrate of leukocytes, significant decrease of the urine concentration, loss of the capacity to concentrate urine as well as fibrosis and apoptosis- are a consequence of a variety of factors that work in complex ways and are still not fully understood. Mediators as angiotensin II, transforming growth factor-β (TGF-β) and nitric oxide (NO) have been… More >

Arwa Osama NEMER¹, Mohammad Saud AL ANAZI², Ramesa Shafi BHAT^1*, Arjumand S. WARSY³, Zeneb A BABAY⁴, Mohammad H. ADDAR⁴, Jilani SHAIK², Sooad AL-DAIHAN¹

BIOCELL, Vol.42, No.1, pp. 1-6, 2018, DOI:10.32604/biocell.2018.07005

Abstract Genomic instability and mutations caused by increases in oxidative stress during pregnancy can damage the fetoplacental unit and can upshot preterm birth. Oxidative damage to DNA may possibly be involved in etiology of preterm birth (PTB) which can be repaired by DNA repair gene. In the present study, we assessed the association of base excision repair gene family by analyzing the association of single nucleotide polymorphisms and genes expression in 8-oxoguanine glycosylase-1 (OGG1) and apurinic-apyrimidinic endonuclease 1 (APE1) genes with risk of preterm birth in Saudi women. We analyzed genotypes of four single nucleotide polymorphisms (SNPs) (rs1052133, rs293795, rs2072668 and… More >