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  • Open Access

    ARTICLE

    3D Bio-Plotted Composite Scaffold Made of Collagen Treated Hydroxyapatite-Tricalciumphosphate for Rabbit Tibia Bone Regeneration

    Pranav S. Sapkal1*, Abhaykumar M. Kuthe1, Divya Ganapathy2, Shantanu C. Mathankar3, Sudhanshu Kuthe4

    Molecular & Cellular Biomechanics, Vol.13, No.2, pp. 115-136, 2016, DOI:10.3970/mcb.2016.013.131

    Abstract Biphasic calcium phosphate scaffolds with 20/80 HA/TCP ratio were fabricated using the 3D-Bioplotting system to heal critical size defects in rabbit tibia bone. Four different architectures were printed in a layer by layer fashion with lay down patterns viz. (a) 0°– 90°, (b) 0°– 45°– 90°– 135°, (c) 0°–108°– 216° and (d) 0°– 60°– 120°. After high-temperature sintering scaffolds were coated with collagen and were further characterized by (FTIR) Fourier Transform Infrared Spectroscopy, (SEM) Scanning Electron Microscopy, (XRD) X-Ray diffraction, Porosity analysis and Mechanical testing. Scaffold samples were tested for its ability to induce cytotoxicity in Balb/c 3T3 cells at… More >

  • Open Access

    ARTICLE

    Soil Microbial Dynamics Modeling in Fluctuating Ecological Situations by Using Subtractive Clustering and Fuzzy Rule-Based Inference Systems

    Sunil Kr. Jha1, Zulfiqar Ahmad2

    CMES-Computer Modeling in Engineering & Sciences, Vol.113, No.4, pp. 443-459, 2017, DOI:10.3970/cmes.2017.113.443

    Abstract Microbial population and enzyme activities are the significant indicators of soil strength. Soil microbial dynamics characterize microbial population and enzyme activities. The present study explores the development of efficient predictive modeling systems for the estimation of specific soil microbial dynamics, like rock phosphate solubilization, bacterial population, and ACC-deaminase activity. More specifically, optimized subtractive clustering (SC) and Wang and Mendel's (WM) fuzzy inference systems (FIS) have been implemented with the objective to achieve the best estimation accuracy of microbial dynamics. Experimental measurements were performed using controlled pot experiment using minimal salt media with rock phosphate as sole carbon source inoculated with… More >

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