Minghui Cui, Limei Lin, Jie Zhang, Xin Song, Shuo Wang, Jing Dong, Xuelei Zhao, Yuehong Long^*, Zhaobin Xing^*

Phyton-International Journal of Experimental Botany, Vol.91, No.12, pp. 2649-2668, 2022, DOI:10.32604/phyton.2022.021631

Abstract Mevalonate pyrophosphate decarboxylase is a kind of key enzyme in the terpenoid synthesis pathway in Eleutherococcus senticosus. The results of bisulfite sequencing showed that there were three kinds of samples with a low (0.68%), medium (0.72%) and high (0.79%) DNA methylation ratio in the promoter of MDD in E. senticosus, respectively. The transcriptome sequencing results showed that the expression of MDD in E. senticosus was significantly up-regulated in the types with low DNA methylation ratios of MDD (P < 0.05). There was a significant negative correlation between the saponin content in E. senticosus and the DNA methylation ratio of MDD… More >

SHUAI LIU¹, JIANZHONG XU^1,*, TINGSHAN LIU¹, ZHIMING RAO², WEIGUO ZHANG^1,*

BIOCELL, Vol.46, No.10, pp. 2303-2313, 2022, DOI:10.32604/biocell.2022.020899

Abstract In this study, phosphoenolpyruvate and erythrose-4-phosphate are efficiently supplied by collaborative design of Embden-Meyerhof-Parnas (EMP) pathway and pentose phosphate (PP) pathway in Escherichia coli, thus increasing the L-tryptophan production. Firstly, the effects of disrupting EMP pathway on L-tryptophan production were studied, and the results indicated that the strain with deletion of phosphofructokinase A (i.e., E. coli JW-5 ΔpfkA) produced 23.4 ± 2.1 g/L of L-tryptophan production. However, deletion of phosphofructokinase A and glucosephosphate isomerase is not conducive to glucose consumption and cell growth, especially deletion of glucosephosphate isomerase. Next, the carbon flux in PP pathway was enhanced by introduction of… More >

Ying Chen^1,#, Chuanqiang Liu^1,#, Qingqing He¹, Jianjian Li², Jingjing Wang², Ling Li², Xiang Yao², Shenghao Zhou³, Haoran Wang^2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 651-665, 2022, DOI:10.32604/phyton.2022.017633

Abstract As a macronutrient, Phosphorus (P) takes many roles in plant growth and development. It should be significant to explore the molecular mechanism of low-phosphorus stress response of plants. Phosphate starvation response (PHR) transcription factors play important roles in response to phosphorus deficiency stress in plants. In this study, we isolated a gene related to the plant phosphorus signaling system from the acid-soil-resistant centipedegrass (Eremochloa ophiuroides [Munro] Hack.), termed EoPHR2. The subcellular localization of EoPHR2 protein was observed to be nuclear located. The expression patterns of EoPHR2 in different tissues and Al/Pi-stress conditions were analyzed by qRT-PCR, they suggested a potential… More >

Fazli Wahid¹, Muhammad Sharif², Shah Fahad³, Amjad Ali⁴, Muhammad Adnan¹, Rafiullah¹, Shah Saud⁵, Subhan Danish^6,*, Muhammad Arif Ali⁶, Niaz Ahmed⁶, Hüseyin Arslan⁷, Doğan Arslan⁸, Murat Erman⁸, Ayman EL Sabagh^8,9,*, Fatemeh Gholizadeh¹⁰, Rahul Datta¹¹

Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 257-278, 2022, DOI:10.32604/phyton.2022.016512

Abstract Phosphorus (P) is a critical nutrient that plays an essential role in improving soil fertility for optimum plant growth and productivity. It is one of the most deficient macro-nutrients in agricultural soils after nitrogen and is considered inadequate for plant growth and production. To P availability in soils, the farmers are applying huge amounts of synthetic P fertilizers that adversely affect the wider environment, groundwater, soil fertility and microbial population. Many beneficial microbes are known to release and supply soluble P for improving growth and yield of a variety of plants in a sustainable manner in P deficient soils. Thus,… More >

HAZAEL RAMIRO CEJA-GALVEZ¹, ERANDIS DHENI TORRES-SÁNCHEZ¹, JUAN HERIBERTO TORRES-JASSO², EMMANUEL REYESURIBE¹, JOEL SALAZAR-FLORES^1,*

BIOCELL, Vol.45, No.5, pp. 1241-1250, 2021, DOI:10.32604/biocell.2021.015771

Abstract Paraoxonase-1 (PON-1) is an enzyme that hydrolyzes organophosphate pesticides. The presence of polymorphisms in PON-1 (L55M and Q192R) decreases its enzyme activity and increases the risk of central nervous system (CNS) toxicity in occupationally exposed farmers, leading to chronic degenerative diseases and death. We studied 103 farmers in the region of Cienega Jalisco, Mexico, which were exposed mainly to organophosphate pesticides. We used serum and plasma samples to assay PON-1 activity and perform polymorphism analysis (L55M and Q192R) using qPCR and TaqMan probes, respectively. For both polymorphisms, there was high percentage of heterozygosity (55 LL = 0.19, LM = 0.75,… More >

Qionglin Luo¹, Mingliang Wang², Hui Zhang¹, Yuejun Ouyang¹, Hongwei Lin¹, You Shu^1,*, Shengpei Su^1,2,*

Journal of Renewable Materials, Vol.9, No.12, pp. 2067-2076, 2021, DOI:10.32604/jrm.2021.016255

Abstract Poly(L-lactic acid) (PLLA) is a thermoplastic material with complete degradability, high biocompatibility and excellent mechanical properties. It can replace petroleum-based polymers are currently being used in the fields of packaging, agriculture, textiles, medical and so on. However, PLLA’s extremely flammability greatly limits its wider application. An bio-based flame retardant L-APP/PLLA composites was prepared by melt blending of the L-APP and PLLA. The morphology, impact properties, thermal properties and flame retardant properties of composites were investigated by field emission scanning electron microscope (SEM), impact tester, differential scanning calorimeter (DSC), thermogravimetric analyzer (TGA), limiting oxygen indexer (LOI) and horizontalvertical burning tester. The… More > Graphic Abstract

Griselda Madrid-Delgado^1,#, Marcos Orozco-Miranda^1,#, Mario Cruz-Osorio^1,#, Ofelia Adriana Hernández-Rodríguez¹, Raúl Rodríguez-Heredia², Melchor Roa-Huerta², Graciela Dolores Avila-Quezada^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.5, pp. 1321-1338, 2021, DOI:10.32604/phyton.2021.016174

Abstract

This review highlights the key role that mycorrhizal fungi play in making phosphorus (Pi) more available to plants, including pathways of phosphorus absorption, phosphate transporters and plant-mycorrhizal fungus symbiosis, especially in conditions where the level of inorganic phosphorus (Pi) in the soil is low. Mycorrhizal fungi colonization involves a series of signaling where the plant root exudates strigolactones, while the mycorrhizal fungi release a mixture of chito-oligosaccharides and liposaccharides, that activate the symbiosis process through gene signaling pathways, and contact between the hyphae and the root. Once the symbiosis is established, the extraradical mycelium acts as an extension of the… More >

You Shu^1,2, Qionglin Luo², Hongwei Lin^2,*, Yuejun Ouyang², Xiaomin Zhang^1,2, Liping Sheng¹, Shengpei Su^1,2,*

Journal of Renewable Materials, Vol.9, No.5, pp. 909-921, 2021, DOI:10.32604/jrm.2021.014216

Abstract Polyvinyl alcohol (PVA) has been widely used in the fields of medical, food and packaging due to its excellent biocompatibility, good fiber-forming and film-forming properties. However, the high flammability of PVA has greatly limited its wider applications. The flame-retardant PVA was prepared by melt blending of a bio-based flame retardant (prepared from lignin, phosphoric acid and carbamide) with thermoplastic PVA (TPVA). The chemical structure, morphology, thermal properties, mechanical properties, fire property and fluidity of this flame retardant PVA were investigated by Fourier transform infrared spectrometer(FTIR), field emission scanning electron microscope(SEM), thermogravimetric analyzer(TGA), impact tester, universal testing machine, horizontal-vertical burning tester,… More >

ZHIHUI WANG¹, YE YUAN^2,*

BIOCELL, Vol.44, No.4, pp. 731-736, 2020, DOI:10.32604/biocell.2020.010575

Abstract Glyphosate is a non-selective broad-spectrum herbicide that blocks plant growth by inhibiting 5- Enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway in microorganisms and plants. The full-length epsps cDNA sequence (paepsps, Genebank: KY860582.1) was cloned and characterized for the first time from Phragmites australis. The full-length cDNA of paepsps was 1308 bp encoding a polypeptide of 435 amino acids. The bioinformatic analyses showed that PaEPSPS has highly homologous with EPSPS from other plants. RT-PCR analysis of paepsps expression indicated that the gene expressed in leaves, stems, and roots, with higher expression in leaves. The expression of the paepsps… More >

HAZAEL RAMIRO CEJA-GÁLVEZ¹, ERANDIS DHENI TORRES-SÁNCHEZ¹, JUAN HERIBERTO TORRES-JASSO², ARTURO VILLALOBOS ORNELAS¹, JOEL SALAZAR-FLORES^1,*

BIOCELL, Vol.44, No.3, pp. 363-370, 2020, DOI:10.32604/biocell.2020.09147

Abstract Paraoxonase-1 (PON1) is an important enzyme in various pathologies such as pesticide poisoning, diabetes, atherosclerosis, neuronal disorders, and cancer, due to its multifunctional activity since it acts on different metabolites. However, one of its main functions is the hydrolysis of organophosphate (OP) compounds from pesticides that cause fatal poisoning at the level of the central nervous system (CNS). The objective of this review was to investigate whether the structure, genetics, and function of PON1 affect the metabolism of organophosphate pesticides or other abnormalities. Information was selected from articles in the database PubMed–NCBI (https://www.ncbi.nlm.nih.gov/ pubmed/) with a publication date between 2011… More >