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  • Open Access

    ARTICLE

    Optimizing Silver Nanoparticle Concentrations to Improve the In Vitro Regeneration and Growth of Phalaenopsis Orchids

    Hay Mon Aung1, Aung Htay Naing2,*, Chang Kil Kim2, Kyeung II Park1,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2719-2727, 2025, DOI:10.32604/phyton.2025.068713 - 30 September 2025

    Abstract Phalaenopsis orchids are economically important ornamental crops; however, their commercial micropropagation is often limited by poor rooting efficiency and inconsistent growth. In this study, we investigated the effects of silver nanoparticles (Ag-NPs) on the in vitro regeneration and growth of Phalaenopsis cultivar 611B to determine the optimal concentration of Ag-NPs for improved micropropagation outcomes. Shoot tip explants (2–3 mm)—derived from protocorm-like bodies were cultured on a regeneration medium containing Hyponex (20:20:20 and 6.5:6.5:19), 18 g/L sugar, 2 g/L peptone, 0.8 g/L activated charcoal, 12.5 g/L potato extract, 50 mL/L apple juice, and 10 mg/L 6-benzylaminopurine (6-BA), with… More >

  • Open Access

    ARTICLE

    Optimizing Ethanol Concentrations to Enhance Growth and Bioactive Compound Production in Agastache rugosa Cultivated in a Plant Factory

    Vu Phong Lam1,2,#, Dao Nhan Loi2,3,#, Xin Wang3, Jongwon Do4,5, Isaya Kisekk5, Nguyen Thi Thanh Nga6, Jongseok Park1,3,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1253-1267, 2025, DOI:10.32604/phyton.2025.062507 - 30 April 2025

    Abstract Agastache rugosa, a medicinal plant known for its bioactive compounds, has gained attention for its pharmacological and commercial potential. This study aimed to optimize ethanol concentration to enhance growth and bioactive compound production in A. rugosa cultivated in a controlled plant factory system. Ethanol treatments at 40 and 80 mM significantly promoted both vegetative and reproductive growth. Plants treated with these concentrations exhibited higher net photosynthetic rates (A) and intercellular CO2 concentration (Ci) compared to the untreated control, whereas stomatal conductance (gs) and transpiration rate (E) remained unaffected. Chlorophyll and carotenoid concentrations, and SPAD values, significantly increased with ethanol… More >

  • Open Access

    ARTICLE

    Salicylic Acid Improved the Growth of Soybean Seedlings by Regulating Water Status and Plant Pigments and Limiting Oxidative Injury under Salinity Stress

    Shahin Imran1,2,*, Md. Asif Mahamud3, Newton Chandra Paul1, Prosenjit Sarker4, Md. Tahjib-Ul-Arif5, Nazmul Islam5, Mohammad Saidur Rhaman6, Saleh H. Salmen7, Sulaiman Ali Alharbi7, Mohammad Javed Ansari8, Mohammed Ali Alshehri9, Akbar Hossain10,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2251-2266, 2024, DOI:10.32604/phyton.2024.055736 - 30 September 2024

    Abstract Soybean (Glycine max) is a potential legume crop, but it cannot thrive in mild salinity. Salicylic acid (SA) is a renowned plant growth hormone that improves tolerance to saline conditions. Hence, the study was performed to understand the functions of priming seeds and supplementation of SA in modulating salt tolerance in soybean seedlings. When exposed to salt stress, soybean seedlings showed considerably higher contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) while having decreased germination and growth factors, water contents, and photosynthetic pigments. The germination rate, final germination percentage, germination index, germination energy, and seed vigor index… More >

  • Open Access

    ARTICLE

    Effects of Different Light Qualities on the Growth Characteristics of Populus trinervis

    Jiaqi Li1,2, Zhensheng Qiao1,2, Dan Zong1,2, Chengzhong He1,2,3,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.5, pp. 1043-1056, 2024, DOI:10.32604/phyton.2024.050637 - 28 May 2024

    Abstract Populus trinervis is native to China and plays an irreplaceable role in maintaining the ecological balance of boreal and temperate forests. P. trinervis mainly grows in high-altitude areas. At present, there are limited studies on the response of P. trinervis to different light qualities, so it is necessary to investigate the photosynthetic physiological changes of P. trinervis in different light environments. In our study, P. trinervis was grown for 8 months under light filtered by three different colored films. The three treatments were blue film, green film, and white plastic film. The effects of blue (B), green (G), and white… More >

  • Open Access

    ARTICLE

    The Impact of Inoculum Preparation Media on Pollutant Removal through Phycoremediation of Agricultural Drainage Water by Desmodesmus sp.

    Asmaa Salah1, Hoda Sany1, Abo El-Khair B. El-Sayed2, Reham M. El-Bahbohy1, Heba I. Mohamed3,*, Ayman Amin1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2875-2890, 2023, DOI:10.32604/phyton.2023.031064 - 15 September 2023

    Abstract Water is the most essential natural resource for the future development. Agriculture production is extensively water-dependent and a significant polluter of water resources. So, this work investigated the effect of two different preparation media [Bold’s Basal Medium (BBM) and Domiati cheese whey (DCW)] for agricultural drainage water (ADW) remediation. All treatments were incubated for 6 days. According to the results of biomass productivity, specific growth rate, photosynthetic pigments, and biochemical composition, Desmodesmus sp. can grow in drainage water without dilution. The two treatments significantly reduced the concentration of nitrate, phosphate, chemical oxygen demand, and sodium in More >

  • Open Access

    ARTICLE

    An in Vitro Approach to Investigate the Role of Abscisic Acid in Alleviating the Negative Effects of Chilling Stress on Banana Shoots

    Ibrahim Hmmam1,*, Ali Raza2, Ivica Djalovic3, Nagwa Khedr1, Abdou Abdellatif1

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1695-1711, 2023, DOI:10.32604/phyton.2023.028317 - 09 March 2023

    Abstract Banana is a tropical crop cultivated in warm places. Chilling stress in Egypt is making banana crops less productive. Abscisic acid (ABA), a key plant hormone, regulates metabolic and physiological processes and protects plants from a variety of stresses. In vitro growing banana shoots were pre-treated with ABA at four concentrations (0, 25, 50, and 100 mM) and chilled at 5°C for 24 h, followed by a six-day recovery period at 25°C. By comparing ABA treatments to both positive and negative controls, physiological and biochemical changes were investigated. Chilling stress (5°C) caused a considerable increase… More >

  • Open Access

    ARTICLE

    Improvement of Selected Morphological, Physiological, and Biochemical Parameters of Banana (Musa acuminata L.) Using Potassium Silicate under Drought Stress Condition Grown in vitro

    Hosny Abdel Aziz1, Mohamed Sharaf2,3, Magdy Omar4, Ahmed Abou El-Yazied5, Nada Ibrahim AlJwaizea6, Shaimaa Ismail5, Mohamed M. A. Omar7, Khadiga Alharbi6,*, Amr Elkelish8,9, Moataz Tawfik10

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1019-1036, 2023, DOI:10.32604/phyton.2023.026769 - 06 January 2023

    Abstract Drought stress has become more common in recent years as a result of climate change impacts on the production of banana crops and other fruit trees. The growth and productivity of Musa spp are severely impacted by the gradual degradation of water resources and the erratic distribution pattern of annual precipitation amount. The aim of the work includes increased drought tolerance in light of water scarcity in the world as a result of the bananas’ being gluttonous for water needs. This investigation was carried out from 2019 to 2020 to study the effect of potassium silicate… More >

  • Open Access

    ARTICLE

    Role of foliar spray of plant growth regulators in improving photosynthetic pigments and metabolites in Plantago ovata (Psyllium) under salt stress–A field appraisal

    ABDUL SAMAD1, KANVAL SHAUKAT1,*, MAHMOOD-UR-REHMAN ANSARI2, MEREEN NIZAR1, NOREEN ZAHRA3, AMBREEN NAZ4, HAFIZ MUHAMMAD WALEED IQBAL5, ALI RAZA6, VLADAN PESIC7, IVICA DJALOVIC8

    BIOCELL, Vol.47, No.3, pp. 523-532, 2023, DOI:10.32604/biocell.2023.023704 - 03 January 2023

    Abstract Salinity is one of the major abiotic factors that limit the growth and productivity of plants. Foliar application of plant growth regulators (PGRs) may help plants ameliorate the negative impacts of salinity. Thus, a field experiment was conducted at the Botanical Garden University of Balochistan, Quetta, to explore the potential role of PGRs, i.e., moringa leaf extract (MLE; 10%), proline (PRO; 1 µM), salicylic acid (SA; 250 µM), and thiourea (TU; 10 mM) in ameliorating the impacts of salinity (120 mM) on Plantago ovata, an important medicinal plant. Salinity hampered plant photosynthetic pigments and metabolites but… More >

  • Open Access

    ARTICLE

    Drought Tolerance in Mung Bean is Associated with the Genotypic Divergence, Regulation of Proline, Photosynthetic Pigment and Water Relation

    Mohammad Rafiqul Islam1, Mohd Mostofa Kamal2, Mohammad Faruk Hossain3, Jamil Hossain4, Mohammad Golam Azam4,11, Mst Masuma Akhter5, M. Kamrul Hasan6, Ibrahim Al-Ashkar7, Khalid F. Almutairi7, Ayman EL Sabagh8,*, Md Atikur Rahman9, Muhammad Aamir Iqbal10, Mohammad Sohidul Islam6,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 955-981, 2023, DOI:10.32604/phyton.2023.025138 - 29 November 2022

    Abstract Drought is one of the critical conditions for the growth and productivity of many crops including mung bean (Vigna radiata L. Wilczek). Screening of genotypes for variations is one of the suitable strategies for evaluating crop adaptability and global food security. In this context, the study investigated the physiological and biochemical responses of four drought tolerant (BARI Mung-8, BMX-08010-2, BMX-010015, BMX-08009-7), and four drought sensitive (BARI Mung-1, BARI Mung-3, BU Mung-4, BMX-05001) mung bean genotypes under wellwatered (WW) and water deficit (WD) conditions. The WW treatment maintained sufficient soil moisture (22% ± 0.5%, i.e., 30% deficit… More >

  • Open Access

    ARTICLE

    Comparative Analysis of the Photosynthetic Characteristics and Active Compounds of Semiliquidambar cathayensis Chang Heteromorphic Leaves

    Xiaoming Tian*, Guangfeng Xiang, Hao Lv, Jing Peng, Lu Zhu

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 837-850, 2023, DOI:10.32604/phyton.2023.024408 - 29 November 2022

    Abstract In the present study, the variation patterns of leaf shape in different populations of individual Semiliquidambar cathayensis plants were analyzed to investigate the relationship among leaf shape variation, photosynthetic properties, and active compounds to understand the genetic characteristics of S. cathayensis and screen elite germplasms. The leaf shape of 18 offspring from three natural S. cathayensis populations was analyzed to investigate the level of diversity and variation patterns of leaf shape. Furthermore, photosynthetic pigment content, physiological parameters of photosynthesis, and the active compounds in leaves of different shapes were determined. Statistical analysis showed that the leaf shape variation in  S.More >

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