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  • Open Access

    ARTICLE

    Optimizing Silver Nanoparticle Concentrations to Improve the In Vitro Regeneration and Growth of Phalaenopsis Orchids

    Hay Mon Aung1, Aung Htay Naing2,*, Chang Kil Kim2, Kyeung II Park1,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2719-2727, 2025, DOI:10.32604/phyton.2025.068713 - 30 September 2025

    Abstract Phalaenopsis orchids are economically important ornamental crops; however, their commercial micropropagation is often limited by poor rooting efficiency and inconsistent growth. In this study, we investigated the effects of silver nanoparticles (Ag-NPs) on the in vitro regeneration and growth of Phalaenopsis cultivar 611B to determine the optimal concentration of Ag-NPs for improved micropropagation outcomes. Shoot tip explants (2–3 mm)—derived from protocorm-like bodies were cultured on a regeneration medium containing Hyponex (20:20:20 and 6.5:6.5:19), 18 g/L sugar, 2 g/L peptone, 0.8 g/L activated charcoal, 12.5 g/L potato extract, 50 mL/L apple juice, and 10 mg/L 6-benzylaminopurine (6-BA), with… More >

  • Open Access

    ARTICLE

    Optimizing Ethanol Concentrations to Enhance Growth and Bioactive Compound Production in Agastache rugosa Cultivated in a Plant Factory

    Vu Phong Lam1,2,#, Dao Nhan Loi2,3,#, Xin Wang3, Jongwon Do4,5, Isaya Kisekk5, Nguyen Thi Thanh Nga6, Jongseok Park1,3,*

    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1253-1267, 2025, DOI:10.32604/phyton.2025.062507 - 30 April 2025

    Abstract Agastache rugosa, a medicinal plant known for its bioactive compounds, has gained attention for its pharmacological and commercial potential. This study aimed to optimize ethanol concentration to enhance growth and bioactive compound production in A. rugosa cultivated in a controlled plant factory system. Ethanol treatments at 40 and 80 mM significantly promoted both vegetative and reproductive growth. Plants treated with these concentrations exhibited higher net photosynthetic rates (A) and intercellular CO2 concentration (Ci) compared to the untreated control, whereas stomatal conductance (gs) and transpiration rate (E) remained unaffected. Chlorophyll and carotenoid concentrations, and SPAD values, significantly increased with ethanol… More >

  • Open Access

    ARTICLE

    Salicylic Acid Improved the Growth of Soybean Seedlings by Regulating Water Status and Plant Pigments and Limiting Oxidative Injury under Salinity Stress

    Shahin Imran1,2,*, Md. Asif Mahamud3, Newton Chandra Paul1, Prosenjit Sarker4, Md. Tahjib-Ul-Arif5, Nazmul Islam5, Mohammad Saidur Rhaman6, Saleh H. Salmen7, Sulaiman Ali Alharbi7, Mohammad Javed Ansari8, Mohammed Ali Alshehri9, Akbar Hossain10,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2251-2266, 2024, DOI:10.32604/phyton.2024.055736 - 30 September 2024

    Abstract Soybean (Glycine max) is a potential legume crop, but it cannot thrive in mild salinity. Salicylic acid (SA) is a renowned plant growth hormone that improves tolerance to saline conditions. Hence, the study was performed to understand the functions of priming seeds and supplementation of SA in modulating salt tolerance in soybean seedlings. When exposed to salt stress, soybean seedlings showed considerably higher contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) while having decreased germination and growth factors, water contents, and photosynthetic pigments. The germination rate, final germination percentage, germination index, germination energy, and seed vigor index… More >

  • Open Access

    ARTICLE

    The Impact of Inoculum Preparation Media on Pollutant Removal through Phycoremediation of Agricultural Drainage Water by Desmodesmus sp.

    Asmaa Salah1, Hoda Sany1, Abo El-Khair B. El-Sayed2, Reham M. El-Bahbohy1, Heba I. Mohamed3,*, Ayman Amin1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2875-2890, 2023, DOI:10.32604/phyton.2023.031064 - 15 September 2023

    Abstract Water is the most essential natural resource for the future development. Agriculture production is extensively water-dependent and a significant polluter of water resources. So, this work investigated the effect of two different preparation media [Bold’s Basal Medium (BBM) and Domiati cheese whey (DCW)] for agricultural drainage water (ADW) remediation. All treatments were incubated for 6 days. According to the results of biomass productivity, specific growth rate, photosynthetic pigments, and biochemical composition, Desmodesmus sp. can grow in drainage water without dilution. The two treatments significantly reduced the concentration of nitrate, phosphate, chemical oxygen demand, and sodium in More >

  • Open Access

    ARTICLE

    Improvement of Selected Morphological, Physiological, and Biochemical Parameters of Banana (Musa acuminata L.) Using Potassium Silicate under Drought Stress Condition Grown in vitro

    Hosny Abdel Aziz1, Mohamed Sharaf2,3, Magdy Omar4, Ahmed Abou El-Yazied5, Nada Ibrahim AlJwaizea6, Shaimaa Ismail5, Mohamed M. A. Omar7, Khadiga Alharbi6,*, Amr Elkelish8,9, Moataz Tawfik10

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1019-1036, 2023, DOI:10.32604/phyton.2023.026769 - 06 January 2023

    Abstract Drought stress has become more common in recent years as a result of climate change impacts on the production of banana crops and other fruit trees. The growth and productivity of Musa spp are severely impacted by the gradual degradation of water resources and the erratic distribution pattern of annual precipitation amount. The aim of the work includes increased drought tolerance in light of water scarcity in the world as a result of the bananas’ being gluttonous for water needs. This investigation was carried out from 2019 to 2020 to study the effect of potassium silicate… More >

  • Open Access

    ARTICLE

    Role of foliar spray of plant growth regulators in improving photosynthetic pigments and metabolites in Plantago ovata (Psyllium) under salt stress–A field appraisal

    ABDUL SAMAD1, KANVAL SHAUKAT1,*, MAHMOOD-UR-REHMAN ANSARI2, MEREEN NIZAR1, NOREEN ZAHRA3, AMBREEN NAZ4, HAFIZ MUHAMMAD WALEED IQBAL5, ALI RAZA6, VLADAN PESIC7, IVICA DJALOVIC8

    BIOCELL, Vol.47, No.3, pp. 523-532, 2023, DOI:10.32604/biocell.2023.023704 - 03 January 2023

    Abstract Salinity is one of the major abiotic factors that limit the growth and productivity of plants. Foliar application of plant growth regulators (PGRs) may help plants ameliorate the negative impacts of salinity. Thus, a field experiment was conducted at the Botanical Garden University of Balochistan, Quetta, to explore the potential role of PGRs, i.e., moringa leaf extract (MLE; 10%), proline (PRO; 1 µM), salicylic acid (SA; 250 µM), and thiourea (TU; 10 mM) in ameliorating the impacts of salinity (120 mM) on Plantago ovata, an important medicinal plant. Salinity hampered plant photosynthetic pigments and metabolites but… More >

  • Open Access

    REVIEW

    Screening and evaluation of chilli (Capsicum annuum L.) genotypes for waterlogging tolerance at seedling stage

    MD. REZWAN MOLLA1,2,4, MD. MOTIAR ROHMAN2,*, MD. ROBYUL ISLAM1,2, MIRZA HASANUZZAMAN3,*, LUTFUL HASSAN4

    BIOCELL, Vol.46, No.7, pp. 1613-1627, 2022, DOI:10.32604/biocell.2022.019243 - 17 March 2022

    Abstract Waterlogging is an illustrious abiotic stress and the constrictions it enforces on plant roots have negative effects on growth and development. This study was undertaken to investigate waterlogging stress tolerant potential in chilli (Capsicum annum L.) genotypes through evaluating morphological, physiological, biochemical and anatomical parameters. Thirty-five days old seedlings of 10 chilli genotypes were exposed to waterlogging stress maintaining water height 3–5 cm over the soil surface artificially for three days. This duration (36–38 DAE) was termed as waterlogging period, and subsequent withdrawal of waterlogging condition (39–45 DAE) was regarded as a recovery phase. Based on… More >

  • Open Access

    ARTICLE

    Proline and Oxidative Metabolism in Young Pecan Trees Associated with Sulphate Accumulation

    Dalila Jacqueline Escudero-Almanza1, Oscar Cruz-Alvarez1, Ofelia Adriana Hernández-Rodríguez1, Juan Luis Jacobo-Cuellar1, Esteban Sánchez-Chávez2, Pablo Preciado-Rángel3, Dámaris Leopoldina Ojeda-Barrios1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.6, pp. 1141-1152, 2022, DOI:10.32604/phyton.2022.019129 - 14 February 2022

    Abstract Pecan [Carya illinoinensis (Wangenh.) K. Koch.] is a deciduous tree whose fruits (nuts) are of high economic value and offer excellent nutritional benefits. However, soils high in sulphates can limit its growth and development. Working with 5-year-old trees of ‘Western Schley’ pecan grown in soils high in sulphates, the levels of proline and oxidative metabolism were recorded in the leaflets. Results showed that different levels of visible leaflet damage (‘sufficiency’, ‘low’, ‘moderate’ or ‘severe’) were associated with different levels of leaflet sulphates (mg kg−1): ‘sufficiency’ (≤40), ‘low’ (41–60), ‘moderate’ (61–80) and ‘severe’ (80–100). ‘Severe’ sulphate damage was… More >

  • Open Access

    ARTICLE

    Changes in Growth, Photosynthetic Pigments, Cell Viability, Lipid Peroxidation and Antioxidant Defense System in Two Varieties of Chickpea (Cicer arietinum L.) Subjected to Salinity Stress

    Zeenat Mushtaq1, Shahla Faizan1, Basit Gulzar2, Humira Mushtaq3, Sayyada Bushra1, Alisha Hussain1, Khalid Rehman Hakeem4,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.1, pp. 149-168, 2022, DOI:10.32604/phyton.2022.016231 - 16 August 2021

    Abstract Salinity is one of the most severe abiotic stresses for crop production. The present study investigates the salinity-induced modulation in growth indicators, morphology and movement of stomata, photosynthetic pigments, activity of carbonic anhydrase as well as nitrate reductase, and antioxidant systems in two varieties of chickpea (Pusa-BG5023, and Pusa-BGD72). On 20th day of sowing, plants were treated with varying levels of NaCl (0, 50, 100, 150 and 200 mM) followed by sampling on 45 days of sowing. Recorded observations on both the varieties reveal that salt stress leads to a significant decline in growth, dry biomass,… More >

  • Open Access

    ARTICLE

    Trichoderma-Induced Improvement in Growth, Photosynthetic Pigments, Proline, and Glutathione Levels in Cucurbita pepo Seedlings under Salt Stress

    Mona H. Soliman1, Taghreed S. Alnusaire2, Nessreen F. Abdelbaky3,4, Aisha A. M. Alayafi5, Mirza Hasanuzzaman6,*, Mohamed M. Rowezak2, Mohamed El-Esawi7, Amr Elkelish8

    Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 473-486, 2020, DOI:10.32604/phyton.2020.08795 - 22 June 2020

    Abstract Salt stress is one of the major abiotic stress in plants. However, traditional approaches are not always efficient in conferring salt tolerance. Experiments were conducted to understand the role of Trichoderma spp. (T. harzianum and T. viride) in growth, chlorophyll (Chl) synthesis, and proline accumulation of C. pepo exposed to salinity stress. There were three salt stress (50, 100, and 150 mM NaCl) lavels and three different Trichoderma inoculation viz. T. harzianum, T. viride, and T. harzianum + T. viride. Salt stress significantly declined the growth in terms of the shoot and root lengths; however, it was improved by the inoculation of Trichoderma spp.… More >

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