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  • Open Access

    ARTICLE

    Putrescine Enhances Seed Germination Tolerance to Heat Stress in Arabidopsis thaliana

    Shiyan Lu, Yulan Hu, Yilin Chen, Yaru Yang, Yue Jin, Ping Li*, Xiangyang Hu*

    Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1879-1891, 2022, DOI:10.32604/phyton.2022.022605

    Abstract Putrescine (Put) as the compound of plant polyamines is catalyzed by arginine decarboxylase (ADC), which is encoded by two members, ADC1 and ADC2 in Arabidopsis, and ADC2 is mainly responsible for Put biosynthesis. Accumulated evidence demonstrates the important function of Put in plant growth and development, but its role in regulating seed germination under high temperature (HT) has not been reported yet. SOMNUS (SOM) is the negative regulator for seed germination thermoinhibition by altering downstream gibberellin (GA) and abscisic acid (ABA) metabolism. In this study, we found exogenous application of Put obviously alleviated the inhibition effect of HT on seed… More >

  • Open Access

    ARTICLE

    Effect of Putrescine on Low-Temperature Acclimation in Chlamydomonas reinhardtii

    Muslum S. Inal1,2, Dilek Unal1,*, Bengu Turkyilmaz Unal3,*, Munir Ozturk4

    Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 583-598, 2022, DOI:10.32604/phyton.2022.018223

    Abstract Putrescine is reported to be necessary for cold acclimation under low-temperature stress. In this study, the effect of low-temperature on some physiological and biochemical parameters has been investigated using the green algae Chlamydomonas reinhardtii. The lipid peroxidation rate, amount of Rubisco protein, activities of antioxidant enzymes and gene expression of polyamine biosynthesis (odc2, and spd1), heat shock proteins (hsp70c, hsp90a, and hsp90c), and PSII repair mechanisms (psba, rep27, and tba1) were determined to understand the low-temperature response. Exogenous putrescine application significantly increased Rubisco protein concentration and catalase enzyme activities under low-temperature stress. Moreover, real-time RT-PCR results and gene expression analysis… More >

  • Open Access

    ARTICLE

    Brief Note : Improved in vitro embryo development of stenospermic grape by putrescine

    MARÍA TERESA PONCE, MÓNICA ELIZABETH GUIÑAZÚ, RICARDO TIZIO

    BIOCELL, Vol.26, No.2, pp. 263-266, 2002, DOI:10.32604/biocell.2002.26.263

    Abstract The goal of this study was to determine the effect of putrescine, added to the culture medium, on the in vitro development of stenospermic grape (Vitis vinifera L) embryos. The cross breedings of Perlón x G.C88552 and Perlón x Argentina were used. 0 (control), 2 and 4 mM of putrescine were added to the immature seed’s culture medium. In Perlón x Argentina, 2mM of putrescine statistically increased the percentage of total embryos, direct germination, polyembryos and normal plants. In Perlón x G.C88552, only 2 mM of putrescine increased all the variables considered, eventually tripling the percentage of normal plants obtained.… More >

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