Xinwen Wang, Fupeng Zhang, Xi Yang, Meiping Xue, Xiaoli Li, Yu Gao, Likun Liu

Oncology Research, Vol.27, No.8, pp. 871-877, 2019, DOI:10.3727/096504018X15426271404407

Abstract Second-generation irreversible epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI), afatinib, has been approved for treating EGFR mutant lung cancer patients, but the mechanism of acquired resistance to afatinib has not been well studied. In this study, we established afatinib acquired resistant cell lines. Gene array technology was used to screen changes in gene expression between afatinib-resistant lung cancer cells and parental cells. Our results showed that secreted phosphoprotein 1 (SPP1) was significantly increased in afatinib-resistant lung cancer cells. To study the effect of SPP1 on afatinib resistance, siSPP1 was used to knock down SSP1 in afatinib-resistant lung cancer… More >

Gaowu Hu, Wei Pen, Ming Wang

Oncology Research, Vol.27, No.4, pp. 439-447, 2019, DOI:10.3727/096504018X15214994641786

Abstract Tripartite motif-containing 14 (TRIM14) is abnormally expressed in several human cancers. However, the function and expression of TRIM14 in human breast cancer are still largely unknown. To understand the biological function of TRIM14 in breast cancer, we measured the expression level of TRIM14. Cell proliferation and cell apoptosis were measured after TRIM14 overexpression or knockdown. Upregulation of TRIM14 was found in human breast cancer specimens and cell lines. Reduction of TRIM14 inhibited cell proliferation but increased cell apoptosis in the BT474 and MDA-MB-231 cell lines. Further study showed that knockdown of TRIM14 upregulated the expression of BAX while downregulating the… More >

Caili Yu, Hao Liu, Jintao Shao, Faai Zhang^*

Journal of Renewable Materials, Vol.10, No.4, pp. 1049-1061, 2022, DOI:10.32604/jrm.2022.017315

Abstract Preparation of polymer microspheres from naturally occurring resource is a challenge. Here, a rosin-based polyol (RAG) was used to prepare polyurethane resin (RPU) firstly, and then act as both self-assembled precursor and emulsifier, rosin based polyurethane microspheres (RPUMs) were prepared. In the process of self-emulsification, the RPU formed vesicles by self-assembly. The outer shell of the vesicle consisted of hydrophilic segments, while the inner shell contained the hydrophobic phase. After cross-linking the RPU and removal of the solvent in the core, the porous-hollow microspheres with pH-sensitive were obtained. The microspheres were characterized by optical microscope (OM), scanning electron microscopy (SEM)… More > Graphic Abstract

XINGYU MEI, ZHOUWEI WU, CHENGZHONG ZHANG, YUE SUN, WEIMIN SHI^*

BIOCELL, Vol.45, No.6, pp. 1551-1559, 2021, DOI:10.32604/biocell.2021.015376

Abstract Vitiligo results in an autoimmune disorder destructing skin pigment cells, melanocytes (Mcs). This study aimed to investigate whether Astragaloside IV (AIV) could efficiently induce differentiation of bone marrow mesenchymal stem cells (BMMSCs) into Mcs. BMMSCs were induced and differentiated into Mcs with 0.1, 0.2, and 0.4 mg/L AIV during 150-day. Morphologic changes of differentiated cells were observed. Levels of some melanocytic specific genes (TRP-1, TRP-2, MART-1, Mitf) were measured with quantitative polymerase chain reaction (qPCR) at 90, 120, and 150 days of induction. After 90-day induction, the differentiated cells with 0.4 mg/L AIV demonstrated the typical morphology of Mcs, positive… More >

SAUD BAWAZER¹, ASGHAR KHAN², ABDUR RAUF^3,*, TAIBI BEN HADDA⁴, YAHYA S. AL-AWTHAN^5,6, OMAR BAHATTAB⁵, UMER RASHID⁷, INAMULLAH KHAN⁸, MUHAMMAD ASIF NAWAZ⁹, MD SAHAB UDDIN^10,11, OLATUNDE AHMED¹², MOHAMMAD ALI SHARIATI¹³

BIOCELL, Vol.45, No.3, pp. 751-759, 2021, DOI:10.32604/biocell.2021.014004

Abstract Protein tyrosine phosphatase 1B (PTP1B) inhibition is considered as a potential therapeutic for the treatment of cancer, type 2 diabetes, and obesity. In our present work, we investigated the anti-diabetic potential of 8-hydroxydiospyrin (8-HDN) from D. lotus against the PTP1B enzyme. It showed significant inhibitory activity of PTP1B with an IC₅₀ value of 18.37 ± 0.02 μM. A detailed molecular docking study was carried out to analyze the binding orientation, binding energy, and mechanism of inhibition. A comparative investigation of 8-HDN in the catalytic, as well as the allosteric site of PTP1B, was performed. Binding energy data showed that compound… More >

Dafni Foti¹, Costas Passialis¹, Elias Voulgaridis¹, Stergios Adamopoulos^2,*

Journal of Renewable Materials, Vol.9, No.1, pp. 85-96, 2021, DOI:10.32604/jrm.2021.011868

Abstract Recycled polystyrene in combination with paraffin wax, alkyd resin, and gum rosin were used as components in formulations to investigate their water repellency when applied to cellulosic filter paper substrates. Polystyrene was used in concentration of 5, 10, 15 and 20%, alkyd resin and gum rosin of 5% each and paraffin wax of 0.5%. Totally, twenty four water repellent solutions were prepared. Water repellency was evaluated in terms of water absorption of the cellulosic fibrous mats. The relations between retention of solid substances of the formulations and grammage and water absorption of filter paper samples were also determined. The results… More >

Qiaoguang Li¹, Sheng Gong¹, Jie Yan¹, Hongchao Hu¹, Xugang Shu^1,2,*, Hanqing Tong^3,*, Zhiye Cai¹

Journal of Renewable Materials, Vol.8, No.3, pp. 289-300, 2020, DOI:10.32604/jrm.2020.08897

Abstract The plasticizer is an important polymer material additive. Non-toxic and environmentally friendly plasticizers are developed recently in order to decrease fossil fuel reserves, serious environmental pollution and the toxicity of phthalate esters. In this study, a new, efficient and environmentally friendly plasticizer of hydrogenated rosin dodecyl ester was prepared by an esterification reaction of hydrogenated rosin and dodecanol. The influences of different reaction conditions (including different catalysts, the catalyst concentration, the ratio of the reactants, reaction temperature, and reaction time) on the esterification yield are examined and discussed. Hydrogenated rosin dodecyl ester with 71.8% yield was synthesized under the optimized… More >

ROBERTO YUNES, PEDRO FERNÁNDEZ, GUSTAVO F. DONCEL, ANÍBAL A. ACOSTA

BIOCELL, Vol.29, No.3, pp. 287-293, 2005, DOI:10.32604/biocell.2005.29.287

Abstract Our objective was to determine the effect of phosphodiesterase (PDE) inhibition on: 1) tyrosine phosphorylation of human spermatozoa at the tail level; and 2) sperm motion parameters and hyperactivated motility. The study was conducted with normozoospermic and asthenozoospermic samples incubated under in vitro capacitating conditions. The main outcome measures were computer-assisted sperm motion analysis and fluorescent immunodetection of phosphotyrosine-containing proteins. Pentoxifylline (PTX) was used as PDE inhibitor because of its wide use in the clinic. PTX-treatment significantly increased sperm velocity, hyperactivated motility and tyrosine-phosphorylation, both in normo and asthenozoospermic samples. Tyrosine-phosphorylation of tail proteins was highly conspicuous in both types… More >

M. I. ROSATTI, M. T. BECONI, M. CÓRDOBA

BIOCELL, Vol.28, No.3, pp. 311-316, 2004, DOI:10.32604/biocell.2004.28.311

Abstract Acrosin activity is associated with normal fertility in human and bovine spermatozoa. The aim of the study was to determine the variation of the enzyme activity in the proacrosin-acrosin system in capacitated and acrosome reacted cryopreserved bovine sperm. Enzyme activity was assessed spectrophotometrically using N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) as specific substrate for acrosin at pH 8. Capacitation with heparin and quercitin failed to induce conversion of proacrosin to acrosin. An increase in acrosin activity produced by the presence of progesterone, in a dose-dependent manner, was related with the induction of true acrosome reaction. The total level of acrosin activity registered showed… More >

R. Yunes, G.F. Doncel, A.A. Acosta

BIOCELL, Vol.27, No.1, pp. 29-36, 2003, DOI:10.32604/biocell.2003.27.029

Abstract Our objective was to study the incidence of sperm-tail phosphotyrosine immunoreactivity in normozoospermic and asthenozoospermic human sperm samples, its association with sperm motion parameters, particularly hyperactivated motility, and its potential involvement in the pathogenesis of asthenozoospermia. The work was conducted as a prospective experimental study in the Sperm Biology and Andrology laboratories of the Jones Institute, a medical school-based fertility center. The study subjects were healthy fertile male donors (normozoospermic samples) and infertile patients (asthenozoospermic samples) attending the center. Recently ejaculated semen samples were washed twice to eliminate seminal plasma and a swim-up was performed to select the motile population… More >