DANILA BOBKOV^1,2,3,*, ANASTASIA POLYANSKAYA¹, ANASTASIA MUSORINA¹, GALINA POLJANSKAYA¹

BIOCELL, Vol.46, No.9, pp. 2053-2058, 2022, DOI:10.32604/biocell.2022.019469

Abstract All non-immortalized mesenchymal stem cells have a limited proliferative potential, that is, replicative senescence (RS) is an integral characteristic of the life of all mesenchymal stem cells (MSCs). It is known that one of the important signs of RS is a decrease of cell motility, and that violations of migration processes contribute to the deterioration of tissue regeneration. Therefore, the characterization of the properties of the cell line associated with RS is a prerequisite for the effective use of MSCs in restorative medicine. One of the key proteins regulating cell motility is the small GTPase RhoA. The main purpose of… More >

JULIANA SOARES^1,2,#, DOUGLAS G. FREITAS^1,3,#, PEDRO S. LOURENÇO^1,4, JEFTE FARIAS^1,5, BRUNO PONTES^1,2,3,4,5,*

BIOCELL, Vol.46, No.9, pp. 2009-2013, 2022, DOI:10.32604/biocell.2022.019969

Abstract Mammalian cell surfaces consist of the plasma membrane supported by an underneath cortical cytoskeleton. Together, these structures can control not only the shape of cells but also a series of cellular functions ranging from migration and division to exocytosis, endocytosis and differentiation. Furthermore, the cell surface is capable of exerting and reacting to mechanical forces. Its viscoelastic properties, especially membrane tension and bending modulus, are fundamental parameters involved in these responses. This viewpoint summarizes our current knowledge on how to measure the viscoelastic properties of cell surfaces employing optical tweezers-based tether assays, paving the way for a better understanding of… More >

Hyun Yoo¹, Kyungyong Chung^2,*

Intelligent Automation & Soft Computing, Vol.34, No.1, pp. 1-13, 2022, DOI:10.32604/iasc.2022.024890

Abstract Human pose estimation has been a major concern in the field of computer vision. The existing method for recognizing human motion based on two-dimensional (2D) images showed a low recognition rate owing to motion depth, interference between objects, and overlapping problems. A convolutional neural network (CNN) based algorithm recently showed improved results in the field of human skeleton detection. In this study, we have combined human skeleton detection and deep neural network (DNN) to classify the motion of the human body. We used the visual geometry group network (VGGNet) CNN for human skeleton detection, and the generated skeleton coordinates were… More >

Batyrkhan Omarov^1,2,3,4,*, Sergazy Narynov¹, Zhandos Zhumanov^1,2, Aidana Gumar^1,5, Mariyam Khassanova^1,5

CMC-Computers, Materials & Continua, Vol.72, No.1, pp. 315-331, 2022, DOI:10.32604/cmc.2022.024566

Abstract In this paper, we propose a skeleton-based method to identify violence and aggressive behavior. The approach does not necessitate high-processing equipment and it can be quickly implemented. Our approach consists of two phases: feature extraction from image sequences to assess a human posture, followed by activity classification applying a neural network to identify whether the frames include aggressive situations and violence. A video violence dataset of 400 min comprising a single person's activities and 20 h of video data including physical violence and aggressive acts, and 13 classifications for distinguishing aggressor and victim behavior were generated. Finally, the proposed method… More >

MARIANO BIZZARRI^*, PAOLA PONTECORVI

BIOCELL, Vol.46, No.6, pp. 1357-1362, 2022, DOI:10.32604/biocell.2022.018471

Abstract The cytoskeleton includes three main classes of networked filaments behaving as a coherent and complex structure that confers stability to cell shape while serving as sensor of internal/extracellular changes. Microenvironmental stimuli interfere with the non-linear dynamics that govern cytoskeleton architecture, namely by fostering symmetry breakings and transitions across different phenotypic states. Such process induces a wholecoherent adaptive response, involving the reprogramming of biochemical and gene-expression patterns. These characteristics are especially relevant during development, and in those conditions in which a deregulated crosstalk between cells and the stroma is at the core of the pathological process. Therefore, studying how the cytoskeleton… More >

Motasem S. Alsawadi^*, Miguel Rio

CMC-Computers, Materials & Continua, Vol.71, No.3, pp. 4643-4658, 2022, DOI:10.32604/cmc.2022.022783

Abstract Action recognition has been recognized as an activity in which individuals’ behaviour can be observed. Assembling profiles of regular activities such as activities of daily living can support identifying trends in the data during critical events. A skeleton representation of the human body has been proven to be effective for this task. The skeletons are presented in graphs form-like. However, the topology of a graph is not structured like Euclidean-based data. Therefore, a new set of methods to perform the convolution operation upon the skeleton graph is proposed. Our proposal is based on the Spatial Temporal-Graph Convolutional Network (ST-GCN) framework.… More >

ANGELES C. TECALCO-CRUZ^*

BIOCELL, Vol.46, No.5, pp. 1209-1213, 2022, DOI:10.32604/biocell.2022.018136

Abstract Cytoskeletal remodeling affects the shape, adhesion, and motility of cells. Cytoskeletal dynamics are modulated by matrix proteins, integrins, and several cytokines in the tumor microenvironment. In this scenario, signaling is activated by integrins and interferons, which can induce ISG15 gene expression. This gene encodes a ubiquitin-like protein that functions as a protein modifier via the ISGylation system. Furthermore, non-conjugated ISG15 acts as a cytokine-like protein. In this viewpoint, the interplay between free ISG15, protein ISGylation, and cytoskeletal dynamics in the tumor microenvironment is discussed. More >

CLAIRE ELIZABETH HILLS, ELEFTHERIOS SIAMANTOURAS, PAUL EDWARD SQUIRES^*

BIOCELL, Vol.46, No.4, pp. 937-940, 2022, DOI:10.32604/biocell.2022.018414

Abstract Changes in cell-cell and cell-substrate adhesion markers are increasingly used to characterize disease onset and progression. However, these relationships depend on both the biochemical and molecular association between cells and between cells and their extracellular matrix, as well as the biophysical and mechanical properties orchestrated by cytoskeletal, membrane and matrix components. To fully appreciate the role of cell adhesion when determining normal physiology and the impact of disease on cellular function, it is important to consider both biochemical and biophysical attributes of the system being investigated. In this short viewpoint we reflect on our experiences assessing cell-cell and/or cell-matrix interactions… More >

M. CAROLINA DI SANTO^1,2,#, AGUSTINA ALAIMO^1,2,#, ANA PAULA DOMÍNGUEZ RUBIO^1,2, OSCAR E. PÉREZ^1,2,*

BIOCELL, Vol.46, No.4, pp. 885-891, 2022, DOI:10.32604/biocell.2022.018391

Abstract Chitosan-based nanocarriers (CS-NCs) show a promising role in improving drugs and bioactive compounds delivery for therapy. However, the effects exerted by CS-NCs at the cellular level, including their recognition and uptake, have not been fully investigated yet. Many factors, including size, shape, concentration, and surface chemistry of CS-NCs, play an important role in determining the types of intracellular signals triggered. The mechanism of uptake and the involvement of the cytoskeleton during the CS-NCs endocytosis variates among the different cell types as well as further effects observed inside cells. In the present work, we discuss the effects induced by CS-NCs per… More >

CINZIA SIGNORINI^*, GIULIA COLLODEL, ELENA MORETTI

BIOCELL, Vol.46, No.4, pp. 881-884, 2022, DOI:10.32604/biocell.2022.018062

Abstract The manuscript deals with our studies and experiences in the assessment of cytoskeleton in different cellular models and situations. The immunofluorescent study of several cytoskeletal proteins was relevant in the evaluation of a therapy for osteoarthritis, in case of alkaptonuria and in testing the efficacy of docetaxel in neuroblastoma cancer cells leading to apoptosis. A relevant part of our experience focus on the study of cytoskeleton in seminiferous epithelium and spermatozoa, identifying alterations affecting blood-testis barrier after a silver nanoparticle treatment, chromosomal segregation in case of varicocele, sperm motility and diagnosing systematic sperm defects as “Primary ciliary dyskinesia” and “Dysplasia… More >