Chunya Hu, Yu He^*

Oncologie, Vol.23, No.2, pp. 241-250, 2021, DOI:10.32604/Oncologie.2021.015828

Abstract Objective: The aim of the present study was to investigate the anti-tumor effects of formononetin on human nonsmall cell lung cancer (NSCLC) and its potential molecular mechanism. Methods: A549 cells were treated with different concentrations of formononetin, then detected the cell proliferation, apoptosis and the expression of HIPK2 respectively by MTT assay, flow cytometry analysis and RT-qPCR. Then the interaction between miR- 27a-3p and its target gene HIPK2 was verified through luciferase reporter assay. The expression of miR-27a- 3p, HIPK2, and p53 was detected after being treated with different formononetin concentrations by RT-qPCR and western blot. Results: The results showed… More >

Adeel Rafiq, Muhammad Afaq, Khizar Abbas, Wang-Cheol Song^*

CMC-Computers, Materials & Continua, Vol.69, No.1, pp. 627-645, 2021, DOI:10.32604/cmc.2021.016529

Abstract The installation of small cells in a 5G network extends the maximum coverage and provides high availability. However, this approach increases the handover overhead in the Core Network (CN) due to frequent handoffs. The variation of user density and movement inside a region of small cells also increases the handover overhead in CN. However, the present 5G system cannot reduce the handover overhead in CN under such circumstances because it relies on a traditionally rigid and complex hierarchical sequence for a handover procedure. Recently, Not Only Stack (NO Stack) architecture has been introduced for Radio Access Network (RAN) to reduce… More >

Jawad Tanveer¹, Amir Haider², Rashid Ali², Ajung Kim^1,*

CMC-Computers, Materials & Continua, Vol.68, No.3, pp. 3807-3823, 2021, DOI:10.32604/cmc.2021.016087

Abstract Drone applications in 5th generation (5G) networks mainly focus on services and use cases such as providing connectivity during crowded events, human-instigated disasters, unmanned aerial vehicle traffic management, internet of things in the sky, and situation awareness. 4G and 5G cellular networks face various challenges to ensure dynamic control and safe mobility of the drone when it is tasked with delivering these services. The drone can fly in three-dimensional space. The drone connectivity can suffer from increased handover cost due to several reasons, including variations in the received signal strength indicator, co-channel interference offered to the drone by neighboring cells,… More >

WEIGAO ZHONG^1,*, AIQIN CHEN², XIAOHONG TANG¹, YI LIU¹

BIOCELL, Vol.45, No.4, pp. 901-910, 2021, DOI:10.32604/biocell.2021.08672

Abstract Earlier studies indicated that circular RNAs (circRNAs) were found in various cancer cells, and circFOXM1 was reported to act as an oncogene in non-small cell lung cancer (NSCLC). However, the function of circFOXM1 in NSCLC remains unclear. The expression levels of genes were measured using quantitative real-time polymerase chain reactions (qRT-PCR). Cell proliferation and apoptosis were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide solution (MTT) and flow cytometry assay. The relative protein expression was assessed by western blot. Moreover, transwell assays were employed to examine cell migration and invasion. The targeted relationship was confirmed by dual-luciferase reporter assay. The expression of circFOXM1 was… More >

DAN ZHANG¹, HAIJING LIU¹, ZHENNAN YI², YUANYUAN LU³, YANYAN CHEN⁴, WEIQIANG SU², HUIBING LIN², ZHIHUI ZHANG^5,*, WEI LEI^6,*

BIOCELL, Vol.45, No.3, pp. 617-625, 2021, DOI:10.32604/biocell.2021.012504

Abstract Chemotherapy drug resistance is the main cause leading to the relapse and metastasis of non-small cell lung cancer (NSCLC) patients. Our study aimed to investigate the mechanism of pemetrexed resistance in NSCLC. Firstly, the pemetrexed (PEM)-resistant PC-9 and A549 lung adenocarcinoma cell lines (PC-9/PEM and A549/PEM) were established. The expression of thymidylate synthase (TS) in PC-9/PEM, A549/PEM, A549, and PC-9 cells were analyzed by qRT-PCR and western blot. Then, cell viability, colony formation, migration, and invasion were performed on PEM-resistant cells transfected with TS siRNA. The role of EGFR in PEM resistance of PEM-resistant cells was investigated using EGFR siRNA.… More >

JUNQI GUO^1,2,#, YUN YANG^1,2,#, WEI ZHAO^1,2, ZHONGHAI YAN³, XIA YANG⁴, YUNFEI YAN^1,2, RUIMIN HAO^1,2, JINXIA HU^1,2,*, FEI JIAO^1,2,*

BIOCELL, Vol.45, No.3, pp. 627-638, 2021, DOI:10.32604/biocell.2021.013496

Abstract Increasing evidence indicates that aberrant expressions of some microRNAs are associated with cancer progression. However, the roles and biological mechanisms of miRNA-16-5p in human non-small cell lung cancer (NSCLC) are not to be well studied. Here, we validated that the expression of miR-16-5p was decreased significantly in NSCLC samples and cell lines. The correlation between the clinicopathological features of NSCLC and the miR-16- 5p expression showed that the expression of miR-16-5p in non-small cell lung cancer was linked with the advanced TNM stage, positive lymph node metastasis, with short overall survival (OS). Also, a negative correlation between miR-16-5p and Fermitin… More >

JI ZHU^#, XIYING BO^#, GENGXI JIANG, SHIHUA YAO, TIEJUN ZHAO^*, LING CHEN^*

BIOCELL, Vol.45, No.3, pp. 639-647, 2021, DOI:10.32604/biocell.2021.012847

Abstract Non-small cell lung cancer (NSCLC) cells intake and consume glucose at high efficiency by aerobic glycolysis to maintain robust cell growth and resist cell death. MicroRNAs (miRNAs) have been known to play pivotal roles in NSCLC development partly through mediating glycolysis. However, only a few miRNAs have been experimentally confirmed as critical regulators of glycolysis in NSCLC. TCGA datasets were analyzed to screen for differentially expressed miRNAs between NSCLC and normal tissues. The function of miR-1294-5p was determined in NSCLC cells by cell proliferation, glucose uptake, lactate release, and Extracellular Acidification Rate (ECAR) assays. The target of miR- 1294-5p was… More >

Nan Geng¹, Wenxia Hu¹, Zhikun Liu², Jingwei Su², Wenyu Sun³, Shaonan Xie⁴, Cuimin Ding^1,*

Oncologie, Vol.22, No.4, pp. 245-254, 2020, DOI:10.32604/oncologie.2020.014125

Abstract Objective: This study was set out to inquire into the expression and clinical significance of lncRNA activated by transforming growth factor β (LncRNA-ATB) and in cancer tissues of patients with non-small cell lung cancer (NSCLC). Methods: LncRNA-ATB in cancer tissues and adjacent tissues of 89 NSCLC patients was detected by quantitative real-time polymerase chain reaction (qRT-PCR), and its clinical diagnostic value in NSCLC was determined by receiver operating characteristic (ROC) curves. Based on the median expression of LncRNAATB in NSCLC tissues, 89 patients were allocated into high- and low-expression groups. The 3-year survival rate was calculated using Kaplan-Meier method and… More >

Binbin QIAN¹, Xiaoduo LIU², Xiaolin GU¹, Lu YANG³, Dake CHEN^1,*

BIOCELL, Vol.44, No.1, pp. 19-26, 2020, DOI:10.32604/biocell.2020.07859

Abstract Prolylcarboxypeptidase (PRCP) belongs to the S28 family of proteases, which is also a dipeptidyl peptidase. In this study, we demonstrate the expression pattern of PRCP in Non-small cell lung cancer (NSCLC). We found that the repression of PRCP expression by small interfering RNA successfully inhibited cell proliferation, migration, and invasion. Further, we explored the involvement of PRCP in the regulation of epithelial-mesenchymal transition (EMT). The epithelial marker E-cadherin was significantly increased, meanwhile mesenchymal markers MUC1, vimentin, and SNAIL were markedly decreased in PRCP knockdown cells. Moreover, the downregulation of PRCP in the NSCLC cells induced the expression of apoptosis-related proteins… More >

Dairong LI¹, Xianlu ZHUO^1,2, Lumi HUANG¹, Xiaohui JI¹, Donglin WANG¹

BIOCELL, Vol.43, No.3, pp. 139-144, 2019, DOI:10.32604/biocell.2019.06460

Abstract X-ray repair cross-complementing protein 1 (XRCC1) could repair cisplatin-induced DNA damage. XRCC1 Arg399Gln and Arg194Trp variants alter XRCC1 expression and function, leading to changes in cancer sensitivity to cisplatin treatment. This study aimed to investigate the effects of XRCC1 Arg399Gln and Arg194Trp polymorphisms on cell viability, apoptosis and XRCC1 expression in cisplatin-sensitive A549 and cisplatin-resistant A549/DDP nonsmall cell lung cancer (NSCLC) cells. Plasmids carrying XRCC1 Arg399Gln and Arg194Trp were constructed and transfected into A549 and A549/DDP cells. RT–PCR, Western blot, MTT assay, and flow cytometry analysis were performed to assess cell viability, apoptosis, and XRCC1 expression. Compared to control cells,… More >