Xiaodong Shi^1,*, Yue Qi¹, Liangzhu Lin¹, Jia Wang¹, Xiaobo Qin², Bei Niu^3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3149-3162, 2023, DOI:10.32604/phyton.2023.027324

Abstract Tartary buckwheat (Fagopyrum tataricum) is a dual-purpose medicinal and food crop grown for its high contents of functional compounds and abundant nutrients. Although studies have shown the differences of total flavonoid content in Tartary buckwheat at different ploidy levels, the composition of flavonoid and its regulatory mechanisms are largely unknown. In this study, the leaf metabolome and transcriptome of diploid and tetraploid accessions of Tartary buckwheat were analyzed to gain insight into the impact of polyploidization on comparative secondary metabolite composition and molecular regulatory mechanism. Based on a widely targeted metabolomics analysis, a total of 792 metabolites were identified, including… More >

Danlei Song^#, Xiaona Yu^#, Yaoyao Li, Xianheng Wang, Xinyuan Cui, Tong Si, Xiaoxia Zou, Yuefu Wang, Minglun Wang, Xiaojun Zhang^*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 439-453, 2023, DOI:10.32604/phyton.2022.023165

Abstract The high-quality genomes and large-scale full-length cDNA sequences of allotetraploid peanuts have been sequenced and released, which has accelerated the functional genomics and molecular breeding research of peanut. In order to understand the difference in the transcriptional levels of wild and cultivated peanuts. In this study, we integrated of second- and third-generation sequencing technologies to sequence full-length transcriptomes in peanut cv. Pingdu9616 and its putative ancestor Arachis monticola. The RNA extracted from six different tissues (i.e., roots, stems, leaves, flowers, needles and pods) were sampled at 20 days after flowering. A total of 31,764 and 33,981 high-quality transcripts were obtained… More >

Xiaodong Shi^1,*, Cong Wang¹, Wenjian Li¹, Hanlei Xiao¹, Yanjie Li¹, Bei Niu^2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1973-1986, 2022, DOI:10.32604/phyton.2022.020065

Abstract Polyploid plants grow well, are stress tolerant, and are rich in nutrients and bioactive compounds. Thus, they are useful for improving crop quality and yield. In this study, we compared the seed characteristics and metabolite profiles of diploid and tetraploid tartary buckwheat, which was developed via an artificially induced chromosome doubling event. The length, width, area, and thousand-grain weight were greater for the tetraploid seeds than for the diploid seeds. However, the germination rate decreased for the tetraploid seeds. Additionally, there was a gap between the shell and kernel of the tetraploid seeds. Moreover, the water absorption rate was higher… More >

Longhua Zhou^1,2,#, Ting He^1,2,#, Jing Li^1,2,3, Guimei Guo^1,2, Yingbo Li^1,2, Hongwei Xu^1,2, Runhong Gao^1,2, Linli Huang^1,2, Yifei Wang^1,2, Ruiju Lu^1,2, Zhiwei Chen^1,2,*, Chenghong Liu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1419-1428, 2022, DOI:10.32604/phyton.2022.019912

Abstract Phytohormones play important roles in plant growth and development, and polyploids are thought to be an important method for plant breeding. However, the relationship between ploidy and phytohormone is still unclear. In this study, barley at three ploidy levels were produced by microspore culture. Therefore, we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and distribution. The results showed that Abscisic acid (ABA), gibberellin (GA), jasmonic acid (JA), auxin (IAA), salicylic acid (SA) and cytokinin (CTK) were successfully determined in shoots and… More >

Marina Tsvetova¹, Lev Elkonin^1,*, Yulia Italianskaya¹

Phyton-International Journal of Experimental Botany, Vol.88, No.4, pp. 389-401, 2019, DOI:10.32604/phyton.2019.07485

Abstract Apomictic seed development is a complex process including formation of unreduced embryo sac, parthenogenetic embryo development from the egg cell, and endosperm formation either autonomously, or due to fertilization of polar nuclei by the sperm (under pseudogamous form of apomixis). In the latter case, an obstacle to the normal endosperm development is disturbance of maternal (m) -to-paternal (p) genomic ratio 2m: 1p that occurs in the cases of pollination of unreduced embryo sac with haploid sperms. Usage of tetraploid pollinators can overcome this problem because in such crosses maternal-to-paternal genomic ratio is 4m: 2p that provides formation of kernels with… More >

KARINA SAYURI UTSUNOMIYA*, MARIA SUELY PAGLIARINI*, CACILDA BORGES DO VALLE**

BIOCELL, Vol.29, No.3, pp. 295-301, 2005, DOI:10.32604/biocell.2005.29.295

Abstract The genus Brachiaria (Trin.) Griseb. has achieved considerable importance to cattle production systems, as a result of the good production and adaptation of a few cultivars to poor and acid soils of the Brazilian savannas. Many of its species and accessions are polyploid and apomictic, which limits direct hybridization. To assist the breeding program, cytogenetic characterization has been undertaken on the accessions of Brachiaria collection at the Embrapa Beef Cattle Research Center. In this study, chromosome number and meiotic behavior are reported for the Brachiaria nigropedata (Ficalho & Hiern) Stapf collection. The 20 available accessions are tetraploid (2n = 4x… More >

Rodríguez de León JI¹, MH Reyes-Valdés², DV Mendoza-Rodríguez³, F Ramírez-Godina², V Robledo-Torres², M Gómez-Martínez², G Hernández-Guzmán⁴

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 101-106, 2015, DOI:10.32604/phyton.2015.84.101

Abstract The cultivated husk tomato (Physalis ixocarpa) (2n = 2x = 24) is native from Mexico and Central America and shows a wide genetic variation. Presently, it is the fourth horticultural crop in cultivation surface in Mexico. The working team of this research previously developed an autotetraploid population by using colchicine. The objectives of the present work were to analyze the ploidy level and meiotic behavior of the subsequent generations (C3, C4, C5, C6) from the original (C2) composed only by plants with the duplicated genome from the Rendidora cultivar, and to determine pollen viability. As a diploid control the cultivar… More >

Wang XL^1,2, Z Zhao¹, JE Quan¹

Phyton-International Journal of Experimental Botany, Vol.80, pp. 93-100, 2011, DOI:10.32604/phyton.2011.80.093

Abstract Robinia pseudoacacia (locust hereafter) is an ornamental tree with various uses. Both homologous tetraploid (tetraploid hereafter) and diploid cultivars are commercially available. The tetraploids have advantageous traits, but poor germinability, and cuttings are difficult to root. Since auxin applications can promote rooting, we evaluated the effects of dipping cuttings in various indole-3-butyric acid (IBA) concentrations for four hours on rooting parameters. IBA significantly affected all measured parameters of both tetraploid and diploids cuttings. Tetraploid cuttings produced no roots without IBA. The effects were strongest at 1000 and 800 mg/L IBA for tetraploids and diploids, respectively. Values for tetraploids and diploids… More >