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  • Open Access

    ARTICLE

    Detection of ROS and translocation of ERP-57 in apoptotic induced human neuroblastoma (SH-SY5Y) cells

    Atif Kamil1, Mubarak Ali Khan1, Muhammad AAsim2, Nadir Zaman Khan2, Raham Sher Khan1, Muhsin Jamal3, Waqar Ahmad4, Mir Azam Khan4, Fazal Jalil4

    BIOCELL, Vol.43, No.3, pp. 167-174, 2019, DOI:10.32604/biocell.2019.06729

    Abstract Several toxic compounds are known to induce apoptosis in mammalian cell lines. The human neuroblastoma cells (SH-SY5Y) were exposed to the phosphatase inhibiting toxin okadaic acid (OA) or hydrogen peroxide (H2O2) to induce apoptosis as well as generate reactive oxygen species (ROS). Mitoxantrone (MXT) was used as a positive control for apoptosis. The SH-SY5Y cells were transfected with eukaryotic expression plasmid pHyPer-dMito encoding mitochondrial-targeted fluorescent or pHyPer-dCito encoding cytoplasmic-targeted fluorescent sensor for hydrogen peroxide (HyPer). The ERp57, also called GRP58 (Glucose-regulated protein 58), is a stress protein induced in conditions like glucose starvation and More >

  • Open Access

    ARTICLE

    Cloning and characterization of 66 kDa streptavidin-binding peptides (SBP) of Pisum sativum L. embryo specific to var. Alaska

    Mahmoud MOUSTAFA1,2 , Saad ALAMRI1, Tarek TAHA3, Ali SHATI1, Sulaiman ALRUMMAN1, Mohamed ALKAHTANI1

    BIOCELL, Vol.43, No.3, pp. 155-166, 2019, DOI:10.32604/biocell.2019.06814

    Abstract The aim of the current research was to clone and to characterize the partial 66 kDa streptavidin-binding peptide (SBP) found in the germinated embryos of Pisum sativum L. var. Alaska. The pea (P. sativum var. Alaska) embryos possess prominent 66 kDa SBPs that gradually disappeared after few hours of germination in germinated embryos, but not in the cotyledons. The total RNA was isolated from embryos of P. sativum but could not be isolated from the cotyledons. The partial nucleotides sequences of 66 kDa SBPs of embryonic stalk (P. sativum var. Alaska) were cloned and identified using pMOSBlue vector.… More >

  • Open Access

    ARTICLE

    Down-regulation of Halr1 during induced differentiation of embryonal carcinoma P19 cells

    Zahra HOSSEININIA1, Sara SOLTANIAN2, Naser MAHDAVI-SHAHRI3, Hesam DEHGHANI1,4

    BIOCELL, Vol.43, No.3, pp. 145-154, 2019, DOI:10.32604/biocell.2019.06468

    Abstract Maintenance of pluripotency depends to diverse regulatory factors. Studies in embryonic stem cells (ESCs) have indicated that large intergenic non-coding RNAs (lincRNAs) are involved in the regulatory network of pluripotency. However, the presence and function of pluripotency-associated lincRNAs in cancer cells with pluripotency features are unknown. In this study, we used embryonal carcinoma (EC) P19 cell lines to investigate the expression level of Halr1 in pluripotency and retinoic acid (RA)-induced differentiated states. Down-regulation of pluripotency associated factors such as OCT4, NANOG, SSEA1 and alkaline phosphatase at transcript and protein levels were used to confirm More >

  • Open Access

    ARTICLE

    XRCC1 Arg399Gln and Arg194Trp polymorphisms regulate XRCC1 expression and chemoresistance of non-small cell lung cancer cells

    Dairong LI1, Xianlu ZHUO1,2, Lumi HUANG1, Xiaohui JI1, Donglin WANG1

    BIOCELL, Vol.43, No.3, pp. 139-144, 2019, DOI:10.32604/biocell.2019.06460

    Abstract X-ray repair cross-complementing protein 1 (XRCC1) could repair cisplatin-induced DNA damage. XRCC1 Arg399Gln and Arg194Trp variants alter XRCC1 expression and function, leading to changes in cancer sensitivity to cisplatin treatment. This study aimed to investigate the effects of XRCC1 Arg399Gln and Arg194Trp polymorphisms on cell viability, apoptosis and XRCC1 expression in cisplatin-sensitive A549 and cisplatin-resistant A549/DDP nonsmall cell lung cancer (NSCLC) cells. Plasmids carrying XRCC1 Arg399Gln and Arg194Trp were constructed and transfected into A549 and A549/DDP cells. RT–PCR, Western blot, MTT assay, and flow cytometry analysis were performed to assess cell viability, apoptosis, and More >

  • Open Access

    ARTICLE

    MicroRNA expression profile and lipid metabolism characteristics in liver of rat undergoing high-fat diet

    Wen JING1,§, Chuanfen LI2,3,§, Yingli LU4, Lianshi FENG4

    BIOCELL, Vol.43, No.3, pp. 129-138, 2019, DOI:10.32604/biocell.2019.06087

    Abstract This study aimed to investigate the microRNA expression profile and the characteristics of lipid metabolism in the livers of rats undergoing a high-fat diet. Fifty male Sprague-Dawley (SD) rats were divided into a standard chow group (C group, N = 10) and a high-fat diet group (H group, N = 40). After 12 weeks, the rat body weight, body length, fat mass, and serum lipid concentration were measured. The expression profile of microRNAs and the gene and protein expression levels involved in lipid metabolism in rat liver were detected. Body fat and serum lipid… More >

  • Open Access

    REVIEW

    The antitumor effects of Newcastle disease virus on glioma

    Ji SHI; Peixin SUN, Ye ZHANG, Bing YAO, Haozhe PIAO

    BIOCELL, Vol.43, No.3, pp. 119-128, 2019, DOI:10.32604/biocell.2019.07577

    Abstract Glioma is the most common primary malignant brain tumor with a poor survival rate. In recent years, no significant progress has been made in the treatment of gliomas in contrast to the development of improved diagnosis via molecular typing. Newcastle disease virus (NDV), a negative-stranded RNA virus that exhibits oncolytic activity, has been investigated for its capacity to elicit antitumor activity in many types of cancers, including glioma. Therefore, application of oncolytic viruses, such as NDV, as a new treatment strategy to specifically target aberrant signaling in glioblastomas has brought new hope. For many years, More >

  • Open Access

    REVIEW

    mRNA-specific translational regulation in yeast

    Bengü ERGÜDEN

    BIOCELL, Vol.43, No.3, pp. 103-118, 2019, DOI:10.32604/biocell.2019.06517

    Abstract The expression of a gene is governed at various levels, from transcriptional to translational level. The translational control is widely used to regulate gene expression, especially when a rapid, local, and selective control over protein synthesis is required. The present review describes instructive examples of translational regulation in yeast, together with regulatory elements within mRNAs. The review also outlines the important contributions of mRNAbinding proteins that act in harmony with several translational elements to generate appropriate translational signals and responses. More >

  • Open Access

    ARTICLE

    Hyaluronic acid inhibited the upregulation of heat shock protein 70 in human chondrocytes from osteoarthritis and Kashin-Beck disease

    Zongqiang GAO1, Xiong GUO2, Junchang CHEN1, Chen DUAN2

    BIOCELL, Vol.43, No.2, pp. 99-102, 2019, DOI:10.32604/biocell.2019.06520

    Abstract This study aimed to investigate the effect of hyaluronic acid (HA) on the expression of heat shock protein 70 (HSP70) in chondrocytes isolated from patients with osteoarthritis (OA) and Kashin-Beck disease (KBD). The chondrocytes were collected from OA and KBD patients, and chondrocytes isolated from patients of accident injuries were used as the control. The chondrocytes were treated with HA at different doses. HSP70 expression in chondrocytes at both mRNA and protein levels was tested by PCR and Western blot analysis. Compared with control, both mRNA and protein levels of HSP70 were higher in chondrocytes More >

  • Open Access

    ARTICLE

    Interaction of IL-22/IL-22R1 promotes cell proliferation and suppresses apoptosis of colorectal cancer via phosphorylation of STAT3

    Xiaoning Qin1, Liqing Yuan2, Hongxun Ruan1, Lin Lin1

    BIOCELL, Vol.43, No.2, pp. 89-98, 2019, DOI:10.32604/biocell.2019.06352

    Abstract Interleukin-22 (IL-22) is a member of IL-10 cytokine family which is expressed in activated T cells predominantly and in activated natural killer cells at lower levels. Previous studies have demonstrated the link between elevated levels of IL-22 and disease severity of psoriasis, Crohn’s disease, rheumatoid arthritis and interstitial lung diseases. However, the function of IL-22 in the development and progression of colorectal cancer (CRC) remains elusive. In this study, we first evaluated the IL-22/IL-22R1 level in CRC patients, and found that tumor tissues have more active expression of IL-22 and IL-22R1 than normal tissues,… More >

  • Open Access

    ARTICLE

    Gene expression of 49 kDa apyrase, cytoskeletal proteins, ATPase, ADPase and amino acid contents of Pisum sativum (L.) cells germinated in Euryops arabicus (Steud. ex Jaub. & Spach) water extract

    Mahmoud MOUSTAFA1,2, Saad ALAMRI1, Hoida ZAKI2, Naglaa LOUTFY2, Tarek TAHA3, Ali SHATI1, Mohamed ALKAHTANI1, Sajda SIDDIQI1

    BIOCELL, Vol.43, No.2, pp. 81-88, 2019, DOI:10.32604/biocell.2019.06231

    Abstract The present research reports of quick and marked changes induced by plant extract of Euryops arabicus in the gene expression of 49-kDa apyrases, cytoskeletal proteins, ATPases, ADPase and amount of amino acid of pea (Pisum sativum L. var. Alaska). Pellets of cytoskeletals proteins (27000 xg) were probed with anti-apyrase antibody, biotinylated anti-rat, actin and alpha and beta-tubulin for Western blotting. ATPase and ADPase activities were determined based on the hydrolytic efficacy of adenine triphosphate and adenine diphosphate. By 72 hours, the abundance of apyrases, cytoskeletal proteins and amount of amino acid in pellets of 27000 xg… More >

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