YUE WANG^1,#, YINPING DUAN^1,#, KUNLIN CHEN³, HUIXIA LI^1,2,*, YAN QUAN^4,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.014305

Abstract Non-alcoholic fatty liver disease is the most common cause of hepatic dysfunction. In the present study, human normal hepatocyte L02 cells were treated with 50% fetal bovine serum to induce the formation of hepatic steatosis in vitro, and then the cells were treated with docosahexaenoic acid to investigate its protective effect on Non-alcoholic fatty liver disease. Our results showed that 50% of fetal bovine serum significantly induced intracellular lipid accumulation and hepatocyte fatty degeneration within 48 h. The expression level of adipose formation-related genes was significantly up-regulated, such as PPARγ, C/EBPα and SREBP-1; meanwhile, the content of cellular total lipid,… More >

LITING YOU^1,#, FEIFEI NA^2,#, JUAN ZHOU³, LIN JIAO³, YI ZHOU^3,*, BINWU YING^3,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.013978

Abstract Reviews The Dectin-1 cluster comprises seven members: CLEC-12A, CLEC-12B, CLEC-1A, CLEC-7A, CLEC- 2, CLEC-9A and OLR1. These members have been demonstrated to be involved in the tumorigenesis, progression, and metastasis of several cancers. However, little is known about their roles in human lung adenocarcinoma (LUAD). The expression patterns of the Dectin-1 cluster were analyzed via the ONCOMINE and GEPIA databases. We evaluated the prognostic value of the Dectin-1 cluster in patients with LUAD using the Kaplan-Meier plotter and GEPIA. Differential expression was validated with the EMBL-EBI database, and protein expression was analyzed with the HPA database. In addition, protein-protein interaction… More >

YUE WANG, YUNFEI ZHENG^*, WEIRAN LI^*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.013960

Abstract Orthodontic tooth movement is triggered by orthodontic force loading on the periodontal ligament and is achieved by alveolar bone remodeling, which is regulated by intimate crosstalk between osteoclastogenesis and osteoblast differentiation. Whether the communication between osteoclasts and osteoblasts is influenced by orthodontic compression stress requires further clarification. In this study, osteoclasts were differentiated for 10 days. On day 4 of differentiation, the number of pre-osteoclasts peaked, as determined by the increased expression of RANK and the number of multinucleated cells. After 24 h of compression stress loading, on day 4, the number of osteoclasts increased, and the optimal magnitude of… More >

MUHAMMAD HAMAYUN^1,*, NASIR KHAN¹, MUHAMMAD NAUMAN KHAN², MUHAMMAD QADIR¹, ANWAR HUSSAIN¹, AMJAD IQBAL³, SUMERA AFZAL KHAN⁴, KHUSHNOOD UR REHMAN⁵, IN-JUNG LEE^6,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.013907

Abstract Bacterial endophytes are beneficial to their hosts as they can fix nitrogen in the soil and make it available to the host. Endophytic bacteria also secrete plant growth-promoting hormones to support their host plants under normal as well as stress conditions. The current study aimed to isolate endophytic bacteria from different parts of Calotropis procera, i.e., roots, stem and leaves of Calotropis procera (Ait.) W.T. Aiton. Plants were collected from the Lundkhwar, district Mardan. A total of 12 bacterial strains, i.e., six from roots, three from the stem and three from the leaves were isolated. The strains were screened for… More >

HONGYUAN ZHANG, MIN ZHANG, JIE TAN, SHUPING HUANG, GUOLIN ZHOU^*, XIA CHEN^*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.013869

Abstract Lagenaria siceraria (Molina) Standley has unique biological characteristics with high nutritional and medicinal values. It is an important pharmaceutical plant with various biologically active ingredients. Genetic improvement and deeper genomic studies require a rich resource of molecular markers. The application of next-generation sequencing technology, especially for transcriptome profiling, has greatly facilitated high throughput single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) discovery. In this study, we sequenced the transcriptome of three major cultivars of L. siceraria and obtained 64.88 GB of clean data. The assembled high-quality reads were clustered into 89,347 unigenes, which were annotated by non-redundant protein database,… More >

YUQING HE¹, LI GUO¹, JUAN DING¹, HAOWEN LV¹, QUANRUI MA¹, CHEN LI¹, YU SHAO¹, QIANG LIU¹, CHUN ZHANG¹, HAO YANG^1,2,*, JUAN LIU^1,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.013842

Abstract Schizophrenia is a common and serious mental illness characterized by severe impairments in thinking, emotions, and behaviors. Usually, the cognitive deficits of schizophrenia are closely associated with abnormal neurogenesis due to the hypofunction of certain neural receptors such as N-methyl-D-aspartate receptors (NMDARs), which mediates neurotransmission. However, little is known about the involvement of NMDAR1 in regulating hippocampal neurogenesis in schizophrenia. In the current study, we present evidence suggesting that NMDAR1 regulates hippocampal neurogenesis as lentivirus-mediated shRNA silencing NMDAR1 gene or blocking with MK-801 results in abnormal neurogenesis consistently found in schizophrenia. The important finding was clearly demonstrated by the multiparametric… More >

MADE DIOUF¹, SARA DIALLO¹, FRANÇOIS ABAYE BADIANE^1,2, OUMAR DIACK¹, DIAGA DIOUF^1,*

BIOCELL, Vol., , DOI: 10.32604/biocell.2021.013706

Abstract Cowpea is one of the most important legume grains in the sub-Saharan region of Africa used for human consumption and animal feed, but its production is hampered by biotic and abiotic constraints raising the need to broaden its genetic basis. For this purpose, seeds of two cowpea varieties Melakh and Yacine were irradiated with 300 and 340 Gy of gamma-ray, respectively. The developed mutant populations were agromorphologically characterized from M5 to M7, while the genetic diversity of the latter was evaluated using 13 ISSR markers. Based on the agromorphological characterization, variation of flower color, pod length, seed coat color, and… More >

JUNQI GUO^1,2,#, YUN YANG^1,2,#, WEI ZHAO^1,2, ZHONGHAI YAN³, XIA YANG⁴, YUNFEI YAN^1,2, RUIMIN HAO^1,2, JINXIA HU^1,2,*, FEI JIAO^1,2,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.013496

Abstract Increasing evidence indicates that aberrant expressions of some microRNAs are associated with cancer progression. However, the roles and biological mechanisms of miRNA-16-5p in human non-small cell lung cancer (NSCLC) are not to be well studied. Here, we validated that the expression of miR-16-5p was decreased significantly in NSCLC samples and cell lines. The correlation between the clinicopathological features of NSCLC and the miR-16- 5p expression showed that the expression of miR-16-5p in non-small cell lung cancer was linked with the advanced TNM stage, positive lymph node metastasis, with short overall survival (OS). Also, a negative correlation between miR-16-5p and Fermitin… More >

SERGIO GASTÓN CASPE¹, JOSÉ LUIS KONRAD², DADIN PRANDO MOORE³, JUAN MANUEL SALA¹, LILIAN LISCHINSKY³, CARLOS MANUEL CAMPERO³, CLAUDIO GUSTAVO BARBEITO^4,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.010701

Abstract Water buffalo (Bubalus bubalis) and domestic cattle (Bos taurus) are closely related species. However, embryo transfer interspecies has been attempted without any success. The failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal interface. Glycosylation patterns have been studied in different species of ruminants; however, in B. bubalis, only the binucleated cells (BNC) have been analyzed. This glycocode is essential for a successful embryo-implantation and can be defined by Lectin-Histochemistry (LHC). The aim of this study is to compare the glycosylation pattern of placenta and uterus in water buffaloes and cattle by LHC. Tissue samples of placenta… More >

TIANXIANG ZHANG^1,#, MEISHAN ZHANG^1,#, MINGHUI SHI^1,#, WEIJIANG JIN^1,#, SHUMIAO ZHANG^2,#, MENGYUAN FAN³, YIMENG LI¹, YUPING MENG², XUELIN TIAN⁴, SHUQIANG LIU^1,*, DEFU HU^1,*

BIOCELL, Vol., , DOI:10.32604/biocell.2021.010277

Abstract Male muskrats (Ondatra zibethicus L.) secrete musk from their scent glands during musk secretion season. Musk plays an important role as a communication pheromone during the breeding season. In this study, gas chromatography–mass spectrometry (GC–MS) was used to analyze the main components of musk. The GC–MS results after methyl esterification showed that 71.55% of the musk is composed of fatty acids. The other components of muskrat musk include cholesterol (9.31%) and other organics. Transcriptome comparison between musk secretion and non-secretion seasons showed significant changes in the scent glands for 53 genes involved in fatty acid and cholesterol synthesis and metabolism… More >