Open Access
ARTICLE
Neural stem cell-conditioned medium upregulated the PCMT1 expression and inhibited the phosphorylation of MST1 in SH-SY5Y cells induced by Aβ25-35
XINWEI WU1, GUOYONG JIA2,*, HONGNA YANG3, CONGCONG SUN2, YING LIU2, ZENGYAN DIAO2
1 Department of Geriatrics, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China
2 Department of Neurology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China
3 Department of Critical-Care Medicine, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China
* Corresponding Author:GUOYONG JIA,
BIOCELL 2022, 46(2), 471-478. https://doi.org/10.32604/biocell.2021.015701
Received 07 January 2021; Accepted 22 March 2021; Issue published 20 October 2021
Abstract
A progressive neurodegenerative disease, Alzheimer’s disease (AD). Studies suggest that highly expressed
protein isoaspartate methyltransferase 1 (PCMT1) in brain tissue. In the current study, we explored the effects of
neural stem cell-conditioned medium (NSC-CDM) on the PCMT1/MST1 pathway to alleviate Aβ
25-35-induced
damage in SH-SY5Y cells. Our data suggested that Aβ25-35 markedly inhibited cell viability. NSC-CDM or Neural
stem cell-complete medium (NSC-CPM) had a suppression effect on toxicity when treatment with Aβ
25-35, with a
greater effect observed with NSC-CDM. Aβ
25-35 + NSC-CDM group exhibited an increase in PCMT1 expression.
sh-PCMT1 markedly decreased cell proliferation and suppressed the protective role of NSC-CDM through the
induction of apoptosis and improved p-MST1 expression. Overexpression of PCMT1 reversed the Aβ
25-35-induced
decrease in cell proliferation and apoptosis. In summary, our findings suggest that NSC-CDM corrects the Aβ
25-35-
induced damage to cells by improving PCMT1 expressions, which in turn reduces phosphorylation of MST1.
Keywords
Cite This Article
WU, X., JIA, G., YANG, H., SUN, C., LIU, Y. et al. (2022). Neural stem cell-conditioned medium upregulated the PCMT1 expression and inhibited the phosphorylation of MST1 in SH-SY5Y cells induced by Aβ
25-35.
BIOCELL, 46(2), 471–478.