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Cantilever Arrays for Multiplexed Mechanical Analysis of Biomolecular Reactions

Min Yue1,1, Jeanne C. Stachowiak1,1,2,2, Arunava Majumdar1,1,3,3
Department of Mechanical Engineering, University of California, Berkeley, CA 94720, USA
Presently at Sandia National Laboratory, Livermore, CA 94551,USA
Materials Sciences Division, Lawrence Berkeley National Labo-]ratory, Berkeley, CA 94720, USA. Corresponding author: majumdar@me.berkeley.edu

Molecular & Cellular Biomechanics 2004, 1(3), 211-220. https://doi.org/10.3970/mcb.2004.001.211

Abstract

Microchips;ontaining arrays of cantilever beams have been used to mechanically detect and quantitatively analyze multiple reactions of DNA hybridization and antigen-antibody binding simultaneously. The reaction-induced deflection of a cantilever beam reflects the interplay between strain energy increase of the beam and the free energy reduction of a reaction, providing an ideal tool for investigating the connection between mechanics and chemistry of biomolecular reactions. Since free energy reduction is common for all reactions, the cantilever array forms a universal platform for label-free detection of various specific biomolecular reactions. A few such reactions and their implications in biology and biotechnology are discussed.

Keywords

Cantilevers, array, DNA hybridization, protein interaction, multiplexing.

Cite This Article

Yue, M., Stachowiak, J. C., Majumdar, A. (2004). Cantilever Arrays for Multiplexed Mechanical Analysis of Biomolecular Reactions. Molecular & Cellular Biomechanics, 1(3), 211–220.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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