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Inhibition of Proliferation, Migration, and Invasion by Knockdown of Pyruvate Kinase-M2 (PKM2) in Ovarian Cancer SKOV3 and OVCAR3 Cells

Yi Miao1, Meng Lu1, Qin Yan, Shuangdi Li, Youji Feng

Department of Obstetrics and Gynecology, Shanghai General Hospital of Nanjing Medical University, Shanghai, P.R. China

Oncology Research 2016, 24(6), 463-475. https://doi.org/10.3727/096504016X14685034103671

Abstract

Pyruvate kinase (PK) is a key enzyme in the process of glycolysis, catalyzing phosphoenolpyruvate (PEP) into pyruvate. Currently, PK isozyme type M2 (PKM2), one subtype of PK, has been proposed as a new tumor marker with high expression in various tumor tissues. Here we aimed to explore the effects of siRNAPKM2 on ovarian carcinoma (OC) cell lines SKOV3 and OVCAR3, in which PKM2 was notably expressed. PKM2 gene interference lentivirus vectors were built by miRNA transfection assay. siRNA-PKM2-transfected SKOV3 and OVCAR3 cells were evaluated for cell proliferation, cell cycle distribution, cell apoptosis, cell migration, and invasion in this study. In addition, the expression levels of several tumor-related genes were measured using real-time PCR and Western blot. Results showed that siRNA-PKM2 markedly inhibited cell proliferation, induced apoptosis, and caused cell cycle arrest at the G0/G1 phase. Cell migration and invasion were significantly suppressed by siRNA-PKM2. Furthermore, the tumor-related genes caspase 7, Bad, and E-cadherin were upregulated, while MMP2, HIF1a, VEGF, and MMP9 were depressed by siRNA-PKM2. The function of siRNA-PKM2 on the biological behavior of OC cells indicated that PKM2 may also be a target for treatment of OC.

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APA Style
Miao, Y., Lu, M., Yan, Q., Li, S., Feng, Y. (2016). Inhibition of proliferation, migration, and invasion by knockdown of pyruvate kinase-m2 (PKM2) in ovarian cancer SKOV3 and OVCAR3 cells. Oncology Research, 24(6), 463-475. https://doi.org/10.3727/096504016X14685034103671
Vancouver Style
Miao Y, Lu M, Yan Q, Li S, Feng Y. Inhibition of proliferation, migration, and invasion by knockdown of pyruvate kinase-m2 (PKM2) in ovarian cancer SKOV3 and OVCAR3 cells. Oncol Res. 2016;24(6):463-475 https://doi.org/10.3727/096504016X14685034103671
IEEE Style
Y. Miao, M. Lu, Q. Yan, S. Li, and Y. Feng "Inhibition of Proliferation, Migration, and Invasion by Knockdown of Pyruvate Kinase-M2 (PKM2) in Ovarian Cancer SKOV3 and OVCAR3 Cells," Oncol. Res., vol. 24, no. 6, pp. 463-475. 2016. https://doi.org/10.3727/096504016X14685034103671



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