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CSTB Downregulation Promotes Cell Proliferation and Migration and Suppresses Apoptosis in Gastric Cancer SGC-7901 Cell Line

Jian Zhang*1, ZhenFeng Shi†1, JinXing Huang, XiaoGuang Zou§

* Department of Cancer Center, The First People’s Hospital of Kashi Prefecture, Kashi Prefecture, Xinjiang, P.R. China
† Department of Urology Surgery Center, The People’s Hospital of Xinjiang Uyghur Autonomous Region, Xinjiang, P.R. China
‡ Department of Urinary Surgery, The People’s Hospital of Shache County, Xinjiang, P.R. China
§ The First People’s Hospital of Kashi Prefecture, Xinjiang, P.R. China
1 These authors are co-first authors in this study.

Oncology Research 2016, 24(6), 487-494. https://doi.org/10.3727/096504016X14685034103752

Abstract

This study aimed to investigate the pivotal role of cystatin B (CSTB) in the development of gastric cancer and to explore its possible regulatory mechanism. Human gastric cancer SGC-7901 cells as a model in vitro were transfected with plasmid PCDNA3.1-CSTB and siRNA-CSTB using Lipofectamine 2000. Quantitative realtime PCR (qRT-PCR) and Western blotting were performed to determine the relative expression of CSTB and PI3K/Akt/mTOR pathway-related protein. Moreover, MTT assay, Transwell assay, and flow cytometry were used to assess cell proliferation, migration, and apoptosis, respectively. The results showed that CSTB was significantly downregulated in SGC-7901 cells compared with gastric epithelial cells. CSTB was successfully overexpressed and suppressed after cells were transfected with pc-CSTB and si-CSTB, respectively. Moreover, cell viability and migration were significantly decreased after being transfected with pc-CSTB when compared with the control group, while being obviously increased after transfection with si-CSTB. However, cell apoptosis was significantly induced after being transfected with pc-CSTB, while being obviously suppressed after transfection with si-CSTB. Besides, the expression levels of p-PI3K, p-Akt, and p-mTOR proteins were all significantly decreased in the pc-CSTB transfection group when compared with the control group, while being increased in the si-CSTB transfection group. Our findings suggest that CSTB downregulation may promote the development of gastric cancer by affecting cell proliferation and migration, and the PI3K/Akt/mTOR signaling pathway was activated in this process. CSTB may serve as a potential therapeutic target for gastric cancer.

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APA Style
Zhang, J., Shi, Z., Huang, J., Zou, X. (2016). CSTB downregulation promotes cell proliferation and migration and suppresses apoptosis in gastric cancer SGC-7901 cell line. Oncology Research, 24(6), 487-494. https://doi.org/10.3727/096504016X14685034103752
Vancouver Style
Zhang J, Shi Z, Huang J, Zou X. CSTB downregulation promotes cell proliferation and migration and suppresses apoptosis in gastric cancer SGC-7901 cell line. Oncol Res. 2016;24(6):487-494 https://doi.org/10.3727/096504016X14685034103752
IEEE Style
J. Zhang, Z. Shi, J. Huang, and X. Zou "CSTB Downregulation Promotes Cell Proliferation and Migration and Suppresses Apoptosis in Gastric Cancer SGC-7901 Cell Line," Oncol. Res., vol. 24, no. 6, pp. 487-494. 2016. https://doi.org/10.3727/096504016X14685034103752



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