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Knockdown of Long Noncoding RNA NR_026689 Inhibits Proliferation and Invasion and Increases Apoptosis in Ovarian Carcinoma HO-8910PM Cells

Xin Zhang*, Shaowen Li, Chunli Dong, Xiuying Xie*, Yunping Zhang*

* Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, P.R. China
† Department of Pediatrics, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, P.R. China
‡ Department of Anesthesiology, The Second Affiliated Hospital of Xi’an Jiaotong University, Xi’an, P.R. China

Oncology Research 2017, 25(2), 259-265. https://doi.org/10.3727/096504016X14732503870766

Abstract

Ovarian cancer is one of the leading causes of gynecological cancer-related deaths worldwide. We investigated the role of a newly discovered long noncoding RNA, NR_026689, in cell proliferation, metastasis, and apoptosis in ovarian cancer cells. Our results showed that NR_026689 was overexpressed in both clinical ovarian cancer patients and cultured ovarian cancer cells. Knockdown of NR_026689 in HO-8910PM cells significantly decreased the cell proliferative rate and the ability to form colonies. Transwell assays revealed that depletion of NR_026689 suppressed cell migration ability by 68% and cell invasive capacity by 71% in HO-8910PM cells. Moreover, specific shRNAs against NR_026689 notably promoted cell apoptosis in HO-8910PM cells by upregulating the expression of proapoptotic proteins, including caspase 3, caspase 9, cytochrome C, and PARP. Our study suggests an oncogenic potential of NR_026689 in ovarian cancer and might provide novel clues for the diagnosis and treatment of ovarian cancer in the clinic.

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APA Style
Zhang, X., Li, S., Dong, C., Xie, X., Zhang, Y. (2017). Knockdown of long noncoding RNA NR_026689 inhibits proliferation and invasion and increases apoptosis in ovarian carcinoma HO-8910PM cells. Oncology Research, 25(2), 259-265. https://doi.org/10.3727/096504016X14732503870766
Vancouver Style
Zhang X, Li S, Dong C, Xie X, Zhang Y. Knockdown of long noncoding RNA NR_026689 inhibits proliferation and invasion and increases apoptosis in ovarian carcinoma HO-8910PM cells. Oncol Res. 2017;25(2):259-265 https://doi.org/10.3727/096504016X14732503870766
IEEE Style
X. Zhang, S. Li, C. Dong, X. Xie, and Y. Zhang, “Knockdown of Long Noncoding RNA NR_026689 Inhibits Proliferation and Invasion and Increases Apoptosis in Ovarian Carcinoma HO-8910PM Cells,” Oncol. Res., vol. 25, no. 2, pp. 259-265, 2017. https://doi.org/10.3727/096504016X14732503870766



cc Copyright © 2017 The Author(s). Published by Tech Science Press.
This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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