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miR-92a Inhibits Proliferation and Induces Apoptosis by Regulating Methylenetetrahydrofolate Dehydrogenase 2 (MTHFD2) Expression in Acute Myeloid Leukemia

Yueli Gu*, Jinchun Si, Xichun Xiao*, Ying Tian*, Shuo Yang*

* Department of Hematology, Shangqiu First People’s Hospital, Shangqiu, P.R. China
† Department of Surgery Teaching and Research Section, Shangqiu Medical College, Shangqiu, P.R. China

Oncology Research 2017, 25(7), 1069-1079. https://doi.org/10.3727/096504016X14829256525028

Abstract

Aberrant expression of microRNA-92a (miR-92a) has been investigated in various cancers. However, the function and mechanism of miR-92a in acute myeloid leukemia (AML) remain to be elucidated. Our data showed that miR-92a was evidently downregulated and methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) was remarkably upregulated in AML cell lines HL-60 and THP-1. Dual luciferase reporter assay revealed that MTHFD2 was a direct target of miR-92a. Gain- and loss-of-function analysis demonstrated that MTHFD2 knockdown or miR-92a overexpression notably inhibited proliferation and promoted apoptosis of AML cell lines. Restoration of MTHFD2 expression reversed proliferation inhibition and apoptosis induction of AML cells triggered by miR-92a. Moreover, an implanted tumor model in mice indicated that miR-92a overexpression dramatically decreased tumor growth and MTHFD2 expression in vivo. Taken together, our results suggest that miR-92a inhibits proliferation and induces apoptosis by directly regulating MTHFD2 expression in AML. miR-92a may act as a tumor suppressor in AML, providing a promising therapeutic target for AML patients.

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APA Style
Gu, Y., Si, J., Xiao, X., Tian, Y., Yang, S. (2017). Mir-92a inhibits proliferation and induces apoptosis by regulating methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) expression in acute myeloid leukemia. Oncology Research, 25(7), 1069-1079. https://doi.org/10.3727/096504016X14829256525028
Vancouver Style
Gu Y, Si J, Xiao X, Tian Y, Yang S. Mir-92a inhibits proliferation and induces apoptosis by regulating methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) expression in acute myeloid leukemia. Oncol Res. 2017;25(7):1069-1079 https://doi.org/10.3727/096504016X14829256525028
IEEE Style
Y. Gu, J. Si, X. Xiao, Y. Tian, and S. Yang "miR-92a Inhibits Proliferation and Induces Apoptosis by Regulating Methylenetetrahydrofolate Dehydrogenase 2 (MTHFD2) Expression in Acute Myeloid Leukemia," Oncol. Res., vol. 25, no. 7, pp. 1069-1079. 2017. https://doi.org/10.3727/096504016X14829256525028



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