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Function of miR-152 as a Tumor Suppressor in Human Breast Cancer by Targeting PIK3CA

Shuke Ge*, Dan Wang, Qinglong Kong, Wei Gao*, Jiayi Sun*

* Second Department of Breast and Thyroid Surgery, Dalian Municipal Central Hospital Affiliated to Dalian Medical University, Dalian, P.R. China
† Department of General Surgery, The Third People’s Hospital of Dalian, Dalian, P.R. China
‡ Department of Thoracic Surgery, Dalian Municipal Central Hospital Affiliated to Dalian Medical University, Dalian, P.R. China

Oncology Research 2017, 25(8), 1363-1371. https://doi.org/10.3727/096504017X14878536973557

14 June 2024 Editors Note:

Readers are alerted that concerns were raised about the integrity of some of the data presented in the article. Further editorial action will be taken once this matter has been resolved.

Abstract

miR-152, as a tumor suppressor, has been reported to be downregulated in a number of cancer cell lines and tumor tissues, including breast cancer. This study aimed to investigate the role of miR-152 in human breast cancer and its underlying mechanisms. Human breast cancer cell line HCC1806 was transfected with hsa-miR- 152-3p mimic, inhibitor, or scrambled negative controls. The efficiency of miR-152-3p transfection was evaluated by quantitative real-time PCR, and the effects on cell viability and apoptosis as well as on the PI3K/AKT signaling pathway were investigated by MTT assay, flow cytometry, and Western blot analysis, respectively. The binding effect of miR-152-3p on PIK3CA 3'-UTR was also investigated. The results suggested that miR- 152-3p mimic transfection inhibited cell viability while inducing apoptosis of HCC1806 cells. Furthermore, miR-152-3p negatively regulated PIK3CA expression via binding to the 3'-UTR of PIK3CA and decreased the phosphorylation levels of AKT (Ser473) and RPS6 (Ser235/236) in HCC1806 cells. miR-152-3p inhibitor transfection showed the opposite effects. In conclusion, miR-152-3p might serve as a tumor suppressor in human breast cancer cells via negatively regulating PIK3CA expression to inhibit the activation of AKT and RPS6, leading to suppression of HCC1806 cell proliferation.

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APA Style
Ge, S., Wang, D., Kong, Q., Gao, W., Sun, J. (2017). Function of mir-152 as a tumor suppressor in human breast cancer by targeting PIK3CA. Oncology Research, 25(8), 1363-1371. https://doi.org/10.3727/096504017X14878536973557
Vancouver Style
Ge S, Wang D, Kong Q, Gao W, Sun J. Function of mir-152 as a tumor suppressor in human breast cancer by targeting PIK3CA. Oncol Res. 2017;25(8):1363-1371 https://doi.org/10.3727/096504017X14878536973557
IEEE Style
S. Ge, D. Wang, Q. Kong, W. Gao, and J. Sun "Function of miR-152 as a Tumor Suppressor in Human Breast Cancer by Targeting PIK3CA," Oncol. Res., vol. 25, no. 8, pp. 1363-1371. 2017. https://doi.org/10.3727/096504017X14878536973557



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