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miR-26b Mimic Inhibits Glioma Proliferation In Vitro and In Vivo Suppressing COX-2 Expression

Zheng-Gang Chen*, Chuan-Yi Zheng*, Wang-Qing Cai, Da-Wei Li*, Fu-Yue Ye*, Jian Zhou*, Ran Wu*, Kun Yang*

* Department of Neurosurgery, The First Affiliated Hospital of Hainan Medical College, Haikou, Hainan, P.R. China
† Department of Neurosurgery, The Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University, Guangzhou, Guangdong, P.R. China

Oncology Research 2019, 27(2), 147-155. https://doi.org/10.3727/096504017X15021536183517

Abstract

Glioma is the most common malignant tumor of the nervous system. Studies have shown the microRNA-26b (miR-26b)/cyclooxygenase-2 (COX-2) axis in the development and progression in many tumor cells. Our study aims to investigate the effect and mechanism of the miR-26b/COX-2 axis in glioma. Decreased expression of miR-26b with increased levels of COX-2 was found in glioma tissues compared with matched normal tissues. A strong negative correlation was observed between the level of miR-26b and COX-2 in 30 glioma tissues. The miR-26b was then overexpressed by transfecting a miR-26b mimic into U-373 cells. The invasive cell number and wound closing rate were reduced in U-373 cells transfected with miR-26b mimic. In addition, COX-2 siRNA enhanced the effect of miR-26b mimic in suppressing the expression of p-ERK1 and p-JNK. Finally, the in vivo experiment revealed that miR-26b mimic transfection strongly reduced the tumor growth, tumor volume, and expression of matrix metalloproteinase-2 (MMP-2) and MMP-9. Taken together, our research indicated a miR-26b/COX-2/ERK/JNK axis in regulating the motility of glioma in vitro and in vivo, providing a new sight for the treatment of glioma.

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APA Style
Chen, Z., Zheng, C., Cai, W., Li, D., Ye, F. et al. (2019). Mir-26b mimic inhibits glioma proliferation in vitro and in vivo suppressing COX-2 expression. Oncology Research, 27(2), 147-155. https://doi.org/10.3727/096504017X15021536183517
Vancouver Style
Chen Z, Zheng C, Cai W, Li D, Ye F, Zhou , et al. Mir-26b mimic inhibits glioma proliferation in vitro and in vivo suppressing COX-2 expression. Oncol Res. 2019;27(2):147-155 https://doi.org/10.3727/096504017X15021536183517
IEEE Style
Z. Chen et al., "miR-26b Mimic Inhibits Glioma Proliferation In Vitro and In Vivo Suppressing COX-2 Expression," Oncol. Res., vol. 27, no. 2, pp. 147-155. 2019. https://doi.org/10.3727/096504017X15021536183517



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