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Upregulation of miR-324-5p Inhibits Proliferation and Invasion of Colorectal Cancer Cells by Targeting ELAVL1

Chijiang Gu*, Mingyuan Zhang*, Weiliang Sun*, Changzheng Dong

* Department of Gastrointestinal Surgery, Affiliated Yinzhou Hospital of Ningbo University, Ningbo City, Zhejiang Province, P.R. China
† School of Medicine, Ningbo University, Ningbo City, Zhejiang Province, P.R. China

Oncology Research 2019, 27(5), 515-524. https://doi.org/10.3727/096504018X15166183598572

Abstract

Colorectal cancer (CRC) is a common clinical cancer that remains incurable in most cases. miRNAs are reported to play a part in the development of various tumors. In the present study, we found that miR-324-5p was downregulated in CRC cells, while ELAV (embryonic lethal, abnormal vision, Drosophila)-like protein 1 (ELAVL1) showed a higher expression. miR-324-5p transfection significantly inhibited the proliferation as well as invasion in both SW620 and SW480 cells. miR-324-5p mimic transfection markedly decreased the expression of ELAVL1. Luciferase reporter gene assay confirmed that ELAVL1 is a direct target of miR- 324-5p. Furthermore, cancer invasion factors uPA, uPAR, and MMP-9 were found to drop significantly in miR-324-5p-transfected groups. To conclude, our findings indicate that miR-324-5p may play a suppressive role in colorectal cell viability and invasion, at least in part, through directly targeting ELAVL1. Therefore, miR-234-5p might function as a promising candidate for CRC treatment and deserves deeper research.

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APA Style
Gu, C., Zhang, M., Sun, W., Dong, C. (2019). Upregulation of mir-324-5p inhibits proliferation and invasion of colorectal cancer cells by targeting ELAVL1. Oncology Research, 27(5), 515-524. https://doi.org/10.3727/096504018X15166183598572
Vancouver Style
Gu C, Zhang M, Sun W, Dong C. Upregulation of mir-324-5p inhibits proliferation and invasion of colorectal cancer cells by targeting ELAVL1. Oncol Res. 2019;27(5):515-524 https://doi.org/10.3727/096504018X15166183598572
IEEE Style
C. Gu, M. Zhang, W. Sun, and C. Dong "Upregulation of miR-324-5p Inhibits Proliferation and Invasion of Colorectal Cancer Cells by Targeting ELAVL1," Oncol. Res., vol. 27, no. 5, pp. 515-524. 2019. https://doi.org/10.3727/096504018X15166183598572



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