Open Access
ARTICLE
NLRP3 Promotes Glioma Cell Proliferation and Invasion via the Interleukin-1b/NF-kB p65 Signals
Liping Xue*1, Bin Lu†1, Bibo Gao‡, Yangyang Shi‡, Jingqi Xu‡, Rui Yang‡, Bo Xu‡, Peng Ding‡
* Department of Ophthalmology, Yunnan No. 2 Provincial People’s Hospital, Kunming, Yunnan, P.R. China
† Department of Neurosurgery, Huzhou Central Hospital, Huzhou, Zhejiang, P.R. China
‡ Department of Neurosurgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, P.R. China
Oncology Research 2019, 27(5), 557-564. https://doi.org/10.3727/096504018X15264647024196
Abstract
Because of the characteristics of high invasiveness, relapse, and poor prognosis, the management of malignant
gliomas has always been a great challenge. Nod-like receptor (NLR) family pyrin domain containing 3 (NLRP3) is
a crucial component of the NLRP3 inflammasome, a multiprotein complex that can trigger caspase 1/interleukin-1
(IL-1)-mediated inflammatory response once activated and participates in the pathogeny of diverse inflammatory
diseases as well as cancers. We examined the function of NLRP3 in the development of glioma. Glioma cells were
treated with NLRP3 interference or overexpression vectors, recombinant IL-1 , IL-1 antibody, and NF- B inhibitor. Cell proliferation and invasion were assessed by CCK-8 and Transwell assays. Gene expression was detected
by PCR, Western blot, and ELISA. NLRP3 and NF- B p65 increased and were positively correlated in glioma
tissues. NLRP3 knockdown suppressed glioma cell growth and invasion with the decrease of IL-1 and NF- B
p65. Conversely, forced expression of NLRP3 promoted cell growth. NLRP3 silencing suppressed ectogenous
IL-1 -elevated cell proliferation and invasion, whereas IL-1 elimination impaired the proproliferation effect of
NLRP3 hyperexpression. Furthermore, NF- B blockage abrogated IL-1 and NLRP3 hyperexpression increased
cell growth and invasion. NLRP3 promoted the growth and invasion of gliomas via the IL-1 /NF- B p65 signals.
Keywords
Cite This Article
Xue, L., Lu, B., Gao, B., Shi, Y., Xu, J. et al. (2019). NLRP3 Promotes Glioma Cell Proliferation and Invasion via the Interleukin-1b/NF-kB p65 Signals.
Oncology Research, 27(5), 557–564. https://doi.org/10.3727/096504018X15264647024196