Table of Content

Open Access iconOpen Access

ARTICLE

NLRP3 Promotes Glioma Cell Proliferation and Invasion via the Interleukin-1b/NF-kB p65 Signals

Liping Xue*1, Bin Lu†1, Bibo Gao, Yangyang Shi, Jingqi Xu, Rui Yang, Bo Xu, Peng Ding

* Department of Ophthalmology, Yunnan No. 2 Provincial People’s Hospital, Kunming, Yunnan, P.R. China
† Department of Neurosurgery, Huzhou Central Hospital, Huzhou, Zhejiang, P.R. China
‡ Department of Neurosurgery, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, P.R. China

Oncology Research 2019, 27(5), 557-564. https://doi.org/10.3727/096504018X15264647024196

Abstract

Because of the characteristics of high invasiveness, relapse, and poor prognosis, the management of malignant gliomas has always been a great challenge. Nod-like receptor (NLR) family pyrin domain containing 3 (NLRP3) is a crucial component of the NLRP3 inflammasome, a multiprotein complex that can trigger caspase 1/interleukin-1 (IL-1)-mediated inflammatory response once activated and participates in the pathogeny of diverse inflammatory diseases as well as cancers. We examined the function of NLRP3 in the development of glioma. Glioma cells were treated with NLRP3 interference or overexpression vectors, recombinant IL-1 , IL-1 antibody, and NF- B inhibitor. Cell proliferation and invasion were assessed by CCK-8 and Transwell assays. Gene expression was detected by PCR, Western blot, and ELISA. NLRP3 and NF- B p65 increased and were positively correlated in glioma tissues. NLRP3 knockdown suppressed glioma cell growth and invasion with the decrease of IL-1 and NF- B p65. Conversely, forced expression of NLRP3 promoted cell growth. NLRP3 silencing suppressed ectogenous IL-1 -elevated cell proliferation and invasion, whereas IL-1 elimination impaired the proproliferation effect of NLRP3 hyperexpression. Furthermore, NF- B blockage abrogated IL-1 and NLRP3 hyperexpression increased cell growth and invasion. NLRP3 promoted the growth and invasion of gliomas via the IL-1 /NF- B p65 signals.

Keywords


Cite This Article

APA Style
Xue, L., Lu, B., Gao, B., Shi, Y., Xu, J. et al. (2019). NLRP3 promotes glioma cell proliferation and invasion via the interleukin-1b/nf-kb p65 signals. Oncology Research, 27(5), 557-564. https://doi.org/10.3727/096504018X15264647024196
Vancouver Style
Xue L, Lu B, Gao B, Shi Y, Xu J, Yang R, et al. NLRP3 promotes glioma cell proliferation and invasion via the interleukin-1b/nf-kb p65 signals. Oncol Res. 2019;27(5):557-564 https://doi.org/10.3727/096504018X15264647024196
IEEE Style
L. Xue et al., "NLRP3 Promotes Glioma Cell Proliferation and Invasion via the Interleukin-1b/NF-kB p65 Signals," Oncol. Res., vol. 27, no. 5, pp. 557-564. 2019. https://doi.org/10.3727/096504018X15264647024196



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 455

    View

  • 461

    Download

  • 0

    Like

Share Link