Open Access
ARTICLE
miR-325-3p Promotes the Proliferation, Invasion, and EMT of Breast Cancer Cells by Directly Targeting S100A2
Huiling Wang*, Xin Hu†, Feng Yang‡, Hui Xiao*
* Department of Surgery, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University),
Changsha, P.R. China
† Department of Surgery, Hunan Children’s Hospital, Changsha, P.R. China
‡ Department of Pharmacy, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University),
Changsha, P.R. China
Oncology Research 2020, 28(7-8), 731-744. https://doi.org/10.3727/096504020X16100888208039
Abstract
This study was designed to investigate the precise mechanisms of miR-325-3p/S100A2 axis in breast cancer
(BC). In this study, we found that the level of miR-325-3p was dramatically increased in BC tissues and cell
lines, and the expression of S100A2 was significantly decreased. Also, the high level of miR-325-3p was
closely associated with low expression of S100A2 in BC tissues. Moreover, introduction of miR-325-3p significantly promoted proliferation, invasion, and EMT of BC cells. Bioinformatics analysis predicted that the
S100A2 was a potential target gene of miR-325-3p. Luciferase reporter assay demonstrated that miR-325-3p
could directly target S100A2. In addition, miR-325-3p overexpression had similar effects with knockdown of
S100A2 on BC cells. Overexpression of S100A2 in BC cells partially reversed the promoted effects of miR-
325-3p mimic. Overexpression of miR-325-3p promoted cell proliferation, invasion, and EMT of BC cells by
directly downregulating S100A2 expression.
Keywords
Cite This Article
Wang, H., Hu, X., Yang, F., Xiao, H. (2020). miR-325-3p Promotes the Proliferation, Invasion, and EMT of Breast Cancer Cells by Directly Targeting S100A2.
Oncology Research, 28(7-8), 731–744.