Open Access
ARTICLE
Potentiality of Different Seed Priming Agents to Mitigate Cold Stress of Winter Rice Seedling
Md. Parvez Anwar1,*, Md. Ariful Islam Khalid1, A. K. M. Mominul Islam1, Sabina Yeasmin1, Sharif Ahmed2, Adel Hadifa3, Ismail A. Ismail4, Akbar Hossain5, Ayman EL Sabagh6,*
1 Agro Innovation Laboratory, Department of Agronomy, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh
2 International Rice Research Institute, Bangladesh Office, Dhaka, 1213, Bangladesh
3 Rice Research and Training Center, Field Crops Research Institute, Agricultural Research Center, Sakha, 33717, Egypt
4 Department of Biology, College of Science, Taif University, Taif, 21944, Saudi Arabia
5 Bangladesh Wheat and Maize Research Institute, Dinajpur, 5200, Bangladesh
6 Department of Agronomy, Faculty of Agriculture, Kafrelsheikh University, Kafr Elsheikh, 33516, Egypt
* Corresponding Authors: Md. Parvez Anwar. Email: ; Ayman EL Sabagh. Email: .e.g
(This article belongs to this Special Issue: Plant Ecophysiology: Recent Trends and Advancements)
Phyton-International Journal of Experimental Botany 2021, 90(5), 1491-1506. https://doi.org/10.32604/phyton.2021.015822
Received 16 January 2021; Accepted 29 March 2021; Issue published 27 May 2021
Abstract
Seed priming has proved to be an effective pre-germination seed invigoration technique for different crops to
improve seed and seedling performance under different abiotic stresses. In Bangladesh, winter rice is very often
exposed to cold waves just after sowing in the nursery bed resulting in poor seed germination and seedling emergence, yellowish and thin seedlings production, and a very low survival rate. Seed priming may mitigate the cold
stress during seed germination and seedling emergence and helps in the quality seedling production of winter rice.
To evaluate the efficacy of different seed priming techniques in increasing seedling emergence, growth, vigor and
survivability of winter rice cultivars under cold stress, a pot experiment was conducted at the Department of Agronomy, Bangladesh Agricultural University during December 2018 to January 2019. The experiment comprised
two factors, (A) Winter rice variety namely, i) BRRI dhan29 and ii) BRRI dhan36; (B) Seed priming agent namely
i) Control (no priming), ii) 20000 ppm NaCl, iii) 30000 ppm NaCl, iv) 20000 ppm KCl, v) 30000 ppm KCl, vi)
20000 ppm CaCl
2, vii) 30000 ppm CaCl
2, viii) 50 ppm CuSO
4, ix) 75 ppm CuSO
4, x) 10000 ppm ZnSO
4, xi)
15000 ppm ZnSO
4, xii) 2 ppm Na
2MoO
4, xiii) 3 ppm Na
2MoO
4, xiv) 100 ppm PEG (Polyethylene glycol
4000) and xv) 150 ppm PEG. Seeds were sown on two different dates viz., 1st December and 1st January so that
seedlings are exposed to cold stress at different stages. The experiment was laid out in a completely randomized
design (CRD) with three replications. Results indicated that (in most of the cases) seed priming has a positive
impact on seedling emergence rate (%), root length, shoot length, root shoot ratio, root dry weight, shoot dry
weight, seedling dry weight and survival rate (%). Among the priming agents, KCl and CaCl
2 performed best;
while priming with NaCl and PEG showed no advantages over no priming for both the sowing dates. In general,
BRRI dhan36 performed better than BRRI dhan29 in terms of seedling growth because of its higher tolerance to
cold stress. But, both the varieties performed similarly in terms of emergence rate and survival rate. Thus, priming
is an effective tool to increase seed germination, better seedling growth, and higher seedling survivability of winter
rice under cold stress, and KCl (20000 ppm) or CaCl
2 (20000 ppm) can be considered as a viable priming agent.
Keywords
Cite This Article
Anwar, M. P., Ariful, M., K., A., Yeasmin, S., Ahmed, S. et al. (2021). Potentiality of Different Seed Priming Agents to Mitigate Cold Stress of Winter Rice Seedling.
Phyton-International Journal of Experimental Botany, 90(5), 1491–1506.