Open Access
ARTICLE
Identification of a Novel OsCYP2 Allele that Was Involved in Rice Response to Low Temperature Stress
Hongxiu Gao1, Lin Zhu2, Tianqi Liu1, Xueyu Leng1, Zhenxing Zhu3, Wei Xie1, Haitao Lv1, Zhengxun Jin1, Ping Wu4,#, Zhongchen Zhang1,*
1
College of Agriculture, Northeast Agricultural University, Harbin, 150030, China
2
Hulin Agricultural Technology Extension Center, Hulin, 158400, China
3
Liaoning Academy of Agricultural Sciences, Shenyang, 110161, China
4
Zhejiang University, Hangzhou, 310000, China
* Corresponding Author: Zhongchen Zhang. Email:
# The author died prior to the submission of this paper. Other authors express their great gratitude and remembrance to him
(This article belongs to this Special Issue: Identification of Genetic/Epigenetic Components Responding to Biotic and Abiotic Stresses in Crops)
Phyton-International Journal of Experimental Botany 2023, 92(6), 1743-1763. https://doi.org/10.32604/phyton.2023.026516
Received 09 September 2022; Accepted 12 December 2022; Issue published 11 April 2023
Abstract
Cyclophilin (CYP) plays an important role in plant response to stress, and
OsCYP2, one gene of cyclophlilin
family, is involved in auxin signal transduction and stress signaling in rice. However, the mechanism that
OsCYP2
is involved in rice response to low temperature is still unclear. We identified a new
OsCYP2 allelic mutant, lrl3,
with fewer lateral roots, and the differences in shoot height, primary root length and adventitious root length
increased with the growth process compared to the wild-type plant. Auxin signaling pathway was also affected
and became insensitive to gravity. The transgenic rice plants with over-expression of
OsCYP2 were more tolerant
to low temperature than the wild-type plants, suggesting that
OsCYP2 was involved in the low temperature
response in rice. In addition,
OsCYP2 negatively regulated the expression of
OsTPS38, a terpene synthase gene,
and was dependent on the
OsCDPK7-mediated pathway in response to low temperature stress.
OsTPS38-
overexpressed transgenic line ox-2 was more sensitive to low temperature. Therefore,
OsCYP2 may negatively
regulate
OsTPS38 through an
OsCDPK7-dependent pathway to mediate the response to low temperature in rice.
These results provide a new basis for auxin signaling genes to regulate rice response to low temperature stress.
Keywords
Supplementary Material
Supplementary Material File
Cite This Article
Gao, H., Zhu, L., Liu, T., Leng, X., Zhu, Z. et al. (2023). Identification of a Novel
OsCYP2 Allele that Was Involved in Rice Response to Low Temperature Stress.
Phyton-International Journal of Experimental Botany, 92(6), 1743–1763.