Open Access

ARTICLE

Proteomics Analysis of Soybean Seedlings under Short-Term Water Deficit

Xiyue Wang, Zihao Wu, Chao Yan, Chunmei Ma, Shoukun Dong^*

College of Agriculture, Northeast Agricultural University, Harbin, 150030, China

* Corresponding Author: Shoukun Dong. Email:

Phyton-International Journal of Experimental Botany 2022, 91(7), 1381-1401. https://doi.org/10.32604/phyton.2022.020251

Received 13 November 2021; Accepted 22 December 2021; Issue published 14 March 2022

Abstract

Soybeans are one of the most important grain crops worldwide. Water deficit, which seriously affects the yield and quality of soybeans, is the main abiotic stress factor in soybean production. As a follow-up study, the droughttolerant soybean variant Heinong 44 was analyzed via proteome analysis. Soybean was exposed to water deficit for 0, 8, and 24 h, and protein samples were extracted for detection of differentially expressed proteins. Protein sequencing of leaf tissues under water stress yielded a total of 549 differentially expressed proteins: 75 and 320 upregulated proteins as well as 70 and 84 downregulated proteins were obtained after 8 and 24 h of water deficit, respectively. Gene Ontology analysis revealed that most of the differentially expressed proteins (DEPs) were involved in catalytic activity, molecular function, and metabolic processes, whereas some of them were involved in photosynthesis, carbon metabolism, and energy metabolism. We also identified some differentially expressed proteins that may be involved in the regulation of water deficit response. Our study provides a theoretical basis for the breeding of drought-resistant soybean varieties.

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