Open Access
ARTICLE
Cloning and Functional Validation of Mung Bean VrPR Gene
Xiaokui Huang1, Yingbin Xue1, Aaqil Khan1, Hanqiao Hu1, Naijie Feng1,2,*, Dianfeng Zheng1,2,*
1
College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China
2
Innovation Research Center for Saline-Alkali Tolerant Rice, Shenzhen Research Institute of Guangdong Ocean University,
Shenzhen, 518120, China
* Corresponding Authors: Naijie Feng. Email: ; Dianfeng Zheng. Email:
Phyton-International Journal of Experimental Botany 2023, 92(8), 2369-2382. https://doi.org/10.32604/phyton.2023.027457
Received 04 November 2022; Accepted 23 April 2023; Issue published 25 June 2023
Abstract
For the purpose of functional validation, the mung bean (
Vigna radiata)
VrPR gene was cloned and overexpressed
in
Arabidopsis thaliana. The findings revealed that the ORF of
VrPR contained 1200 bp, in which 399 amino acids
were encoded. Bioinformatics analysis showed that the
VrPR protein belonged to the NADB Rossmann superfamily, which was one of the non-transmembrane hydrophilic proteins.
VrPR was assumed to have 44 amino acid
phosphorylation sites and be contained in chloroplasts. The
VrPR secondary structure comprised of random coil,
α helix, β angle, and extended chain, all of which were quite compatible with the anticipated tertiary structure.
Moreover, analysis of the phylogenetic tree indicated that the soybean PR (Glyma.12G222200) and
VrPR were
closely related. Furthermore, chlorophyll content in leaves is markedly increased in Arabidopsis when
VrPR is
overexpressed. Our findings will serve as a reference for more functional studies on the PR genes in mung bean.
Keywords
Cite This Article
APA Style
Huang, X., Xue, Y., Khan, A., Hu, H., Feng, N. et al. (2023). Cloning and functional validation of mung bean <i>vrpr </i>gene. Phyton-International Journal of Experimental Botany, 92(8), 2369-2382. https://doi.org/10.32604/phyton.2023.027457
Vancouver Style
Huang X, Xue Y, Khan A, Hu H, Feng N, Zheng D. Cloning and functional validation of mung bean <i>vrpr </i>gene. Phyton-Int J Exp Bot. 2023;92(8):2369-2382 https://doi.org/10.32604/phyton.2023.027457
IEEE Style
X. Huang, Y. Xue, A. Khan, H. Hu, N. Feng, and D. Zheng "Cloning and Functional Validation of Mung Bean <i>VrPR </i>Gene," Phyton-Int. J. Exp. Bot., vol. 92, no. 8, pp. 2369-2382. 2023. https://doi.org/10.32604/phyton.2023.027457