Open Access

ARTICLE

Selection and Verification of Reference Genes for qRT-PCR Analysis in Iris domestica under Drought

Qiang Ai, Cuijing Liu^*, Mei Han^*, Limin Yang
College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun, 130118, China
* Corresponding Authors: Cuijing Liu. Email: ; Mei Han. Email:
(This article belongs to this Special Issue: Plant Omics in Challenging Environment)

Phyton-International Journal of Experimental Botany 2022, 91(11), 2537-2548. https://doi.org/10.32604/phyton.2022.021889

Received 11 February 2022; Accepted 28 March 2022; Issue published 12 July 2022

Abstract

Iris domestica is a plant of the Iridaceae family and is drought-tolerant, but its drought-resistance mechanism is not yet clear. Analysing the gene expression changes of I. domestica by qRT-PCR is an important mean to understand its drought resistance characteristics. Nevertheless, a lack of reference genes greatly hinders investigation and research on the adaptation of I. domestica to drought at the molecular and genetic levels. In this study, we assessed the expression stability of 11 candidate gene in I. domestica under drought stress conditions and different tissues using geNorm, NormFinder, BestKeeper and RefFinder tools. The results showed that EF1β was the most stable reference genes under drought stress and in different tissues. To validate further the stability of the identified reference genes, the expression patterns of VP gene in I. domestica was analysed. These results will be conducive to more accurate quantification of gene expression levels in I. domestica.

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Keywords

Iris domestica; gene expression; qRT-PCR; reference genes; drought

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