Open Access
ARTICLE
Triterpenoids from Uncaria rhynchophylla and Their PTP1B Inhibitory Activity
Shuaibin Luo1, Xuemei Zhang2, Kang He1, Juan Zou1,*, Chang-An Geng2,*
1 Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, China
2 State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming, 650201, China
* Corresponding Authors: Juan Zou. Email: ; Chang-An Geng. Email:
Phyton-International Journal of Experimental Botany 2022, 91(8), 1809-1816. https://doi.org/10.32604/phyton.2022.020935
Received 20 December 2021; Accepted 18 January 2022; Issue published 14 April 2022
Abstract
Uncaria rhynchophylla (Gouteng) is a famous traditional Chinese medicine used for psychiatric and hypotensive purposes in China. In this study, the ethyl acetate (EtOAc) part of
U. rhynchophylla was revealed with protein tyrosine phosphatase 1B (PTP1B) inhibitory activity. Subsequent investigation on the EtOAc part yielded one new triterpenoid, 3
β-formyloxy-6
β,19
α-dihydroxyurs-12-en-28-oic acid (
1) and four known ones, 3
β,6
β,19
α-trihydroxyurs-12-en-28-oic acid (
2), 2-oxopomolic acid (
3), 3
β,19
α-dihydroxy-6-oxo-olean-12-en-28-oic acid (
4) and sumaresinolic acid (
5). The structure of compound
1 was determined by extensive HRESIMS, IR, 1D and 2D NMR spectroscopic analyses. Two ursane-type triterpenoids (
2 and
3) showed selective inhibition on PTP1B with IC
50 values of 48.2 and 178.7
μM. The enzyme kinetic study suggested that compounds
2 and
3 were mix-type inhibitors on PTP1B with
Ki values of 15.6 and 132.5
μM. This investigation manifests the antidiabetic potency of
U. rhynchophylla with triterpenoids as the active constituents.
Keywords
Cite This Article
Luo, S., Zhang, X., He, K., Zou, J., Geng, C. (2022). Triterpenoids from
Uncaria rhynchophylla and Their PTP1B Inhibitory Activity.
Phyton-International Journal of Experimental Botany, 91(8), 1809–1816.