Open Access iconOpen Access

ARTICLE

Effects of Plant Extracts and Beauveria bassiana on the Activity of Defense-Related Enzymes in Solanum lycopersicum L. during Interaction with Fusarium oxysporum f. sp. lycopersici

José Adrian Perez-Robles, Carlos Alberto Lecona-Guzmán*, Víctor Manuel Ruíz-Valdiviezo, Joaquín Adolfo Montes-Molina*

Laboratorio de Biotecnología Vegetal, Tecnológico Nacional de Mexico, Campus Tuxtla Gutiérrez, Tuxtla Gutiérrez, 29050, Mexico

* Corresponding Authors: Carlos Alberto Lecona-Guzmán. Email: email,email; Joaquín Adolfo Montes-Molina. Email: email

Phyton-International Journal of Experimental Botany 2023, 92(9), 2503-2518. https://doi.org/10.32604/phyton.2023.029784

Abstract

The objective to this work was to evaluate the enzymatic activity in the culture of Solanum lycopersicum L. infected with Fusarium oxysporum after the combined application of Beauveria bassiana and plant extracts. Solanum lycopersicum plantlets were transplanted 15 days after the emergency. Five days after transplanting, Beauveria bassiana spores were applied at a concentration of 1 × 107 spores mL−1 onto soil (along with A. indica (N) and P. auritum (H) leaf extracts) where S. lycopersicum plants were planted. Eight days after transplanting, spores of F. oxysporum strain were applied at a concentration of 1 × 106 spores mL−1 to soil where S. lycopersicum plants were growing. The development of S. lycopersicum plants was monitored for 114 days, whereby a randomized complete block treatment design was used, the roots of the plants were crushed with liquid nitrogen, after which UV/VIS spectrophotometry was used to determine protein concentration, as well as the activity of β-1,3-glucanases, peroxidases (POX, EC1.11.1.7), catalase and chitinases. The treatments combining B. bassiana and A. indica and P. auritum extracts, had a significant difference in enzymatic activity levels, thus contributing to better defense mechanisms and a greater protection to S. lycopersicum plants in the presence of F. oxysporum. The application of B. bassiana and plant extracts to the ground mitigated damage caused by F. oxysporum on S. lycopersicum plants.

Keywords


Cite This Article

APA Style
Perez-Robles, J.A., Lecona-Guzmán, C.A., Ruíz-Valdiviezo, V.M., Montes-Molina, J.A. (2023). Effects of plant extracts and beauveria bassiana on the activity of defense-related enzymes in solanum lycopersicum L. during interaction with fusarium oxysporum f. sp. lycopersici. Phyton-International Journal of Experimental Botany, 92(9), 2503-2518. https://doi.org/10.32604/phyton.2023.029784
Vancouver Style
Perez-Robles JA, Lecona-Guzmán CA, Ruíz-Valdiviezo VM, Montes-Molina JA. Effects of plant extracts and beauveria bassiana on the activity of defense-related enzymes in solanum lycopersicum L. during interaction with fusarium oxysporum f. sp. lycopersici. Phyton-Int J Exp Bot. 2023;92(9):2503-2518 https://doi.org/10.32604/phyton.2023.029784
IEEE Style
J.A. Perez-Robles, C.A. Lecona-Guzmán, V.M. Ruíz-Valdiviezo, and J.A. Montes-Molina "Effects of Plant Extracts and Beauveria bassiana on the Activity of Defense-Related Enzymes in Solanum lycopersicum L. during Interaction with Fusarium oxysporum f. sp. lycopersici," Phyton-Int. J. Exp. Bot., vol. 92, no. 9, pp. 2503-2518. 2023. https://doi.org/10.32604/phyton.2023.029784



cc This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • 516

    View

  • 277

    Download

  • 0

    Like

Share Link