Open Access

ARTICLE

Identification of PBL Gene Family in Tree Peonies and Its Function in Regulating Pollen Tube Growth

Yuxin Zhao, Zhanxiang Tan, Yuying Li, Kaiyue Zhang, Lili Guo^*, Xiaogai Hou^*

1 College of Agriculture, Henan University of Science and Technology, Luoyang, 471000, China

* Corresponding Authors: Lili Guo. Email: ; Xiaogai Hou. Email:

(This article belongs to the Special Issue: Ornamental Plants: Traits, Flowering, Aroma, Molecular Mechanisms, Postharvest Handling, and Application)

Phyton-International Journal of Experimental Botany 2025, 94(4), 1159-1176. https://doi.org/10.32604/phyton.2025.063737

Received 22 January 2025; Accepted 14 March 2025; Issue published 30 April 2025

Abstract

Receptor-like cytoplasmic kinases (RLCKs) play a crucial role in the physiological processes of plant growth and development and stress response. To elucidate the characteristics and functions of the PBL gene family in tree peonies, the whole genome identification of PBL family members in tree peonies was conducted using a bioinformatics approach based on the published Arabidopsis thaliana PBL protein sequence. A total of 51 PoPBL members were identified, which were distributed unevenly on five chromosomes in the tree peony. PoPBL proteins were localized in the nucleus, cytoplasm, chloroplasts, and mitochondria, with most members of the same clade exhibiting similar gene structures and conserved motif components. The promoter region of PoPBLs contained many response elements related to plant hormones and stress. The relative expression levels of PoPBL1, PoPBL4, PoPBL14, PoPBL40 and PoPBL45 were highly expressed in the pistil samples from the first hour after pollination, and all of them contained elements related to growth and development. At the same time, PoPBL40 of 2044 bp was obtained, and it was found that it had a positive regulatory effect on pollen tube growth of tree peonies, which laid a foundation for further study of PBL gene function in tree peonies.

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Keywords

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Supplementary Material

Supplementary Material File

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