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The cover image illustrates the crucial role of biochar as an important component of sustainable agriculture because it helps plants cope with heat stress and improves soil microbial communities. Heat stress can have various detrimental effects on a plant's growth, development, and production. High temperatures limit energy production by destroying chloroplast structures and lowering the amount of chlorophyll, which interferes with photosynthesis. Additionally, it alters the activity of enzymes, causes plant toxicity, and changes hormone and gene expression. In the end, it results in lower crop quality and production. Applying biochar to soil enhances soil structure and water retention, producing a more conducive microclimate for plant roots during hot weather. Through improved hydration and nutrient availability, this buffering action lessens plant stress.
Additionally, biochar enhances microbial species' diversity and activity by offering a stable home for beneficial soil microorganisms, by improving nutrient cycling, avoiding disease, and boosting stress tolerance, these microbes, in turn, support plant health. Overall, introducing biochar to agricultural systems helps improve the soil's productivity and fertility in the long run by fostering a resilient soil ecosystem and reducing the negative impacts of heat stress on crops.
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  • Open AccessOpen Access

    REVIEW

    Understanding the Molecular Mechanisms of Nitrogen Assimilation in C3 Plants under Abiotic Stress: A Mini Review

    Saif Ullah1,2, Izhar Ali1,2,*
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1029-1045, 2025, DOI:10.32604/phyton.2025.064608 - 30 April 2025
    (This article belongs to the Special Issue: Metabolic Mechanisms of Plant Responses to Stress)
    Abstract Nitrogen (N) assimilation is crucial for the growth and development of C3 plants, as it converts inorganic N into organic forms, important for protein synthesis, nucleic acids and other vital biomolecules. However, abiotic stressors such as drought, salinity, extreme temperatures and others significantly impact N uptake and utilization, thereby hindering plant growth and development. Recent advances in molecular biology have illuminated the complex networks that govern N assimilation under these stressful conditions, emphasizing the role of transcription factors, regulatory genes, and stress-responsive pathways. This review provides an integrated perspective on the latest research in nitrogen metabolism… More >

  • Open AccessOpen Access

    REVIEW

    Essential Oils Usage on Vitis vinifera L., from the Vineyard to Post-Harvest: Advantages, Limitations, and Future Perspectives

    Pamela Lippi1, Aleš Eichmeier2, Sergio Puccioni3, Giovan Battista Mattii1, Eleonora Cataldo1,*
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1047-1072, 2025, DOI:10.32604/phyton.2025.064272 - 30 April 2025
    (This article belongs to the Special Issue: Adaptation Mechanisms of Grapevines to Growing Environments and Agricultural Strategies)
    Abstract The search for environmentally friendly approaches in viticulture is increasing, driven by the need to minimize the ecological footprint of conventional methods while ensuring high grape quality and stable yields. Among the various alternatives explored, essential oils (EOs) have drawn attention due to their natural origin and bioactive properties, including antimicrobial, antifungal, and insect-repellent effects. They are characterized by numerous utilisations, from managing diseases and pests in vineyards to post-harvest applications to preserve and prolong storage duration. This innovative review examines, for the first time, the topic of EOs on viticulture, embracing their multiple uses… More >

  • Open AccessOpen Access

    REVIEW

    Biochar Amendments in Soil: A Sustainable Approach for Mitigating Heavy Metal Stress in Plants

    Shahin Imran1,2,#,*, Mousumi Jahan Sumi3,#, Israt Jahan Harine4, Newton Chandra Paul1, Md. Asif Mahamud5, Rakibul Hasan Md. Rabbi5, Marian Brestic6, Mohammad Saidur Rhaman7,*
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1073-1109, 2025, DOI:10.32604/phyton.2025.064046 - 30 April 2025
    (This article belongs to the Special Issue: Plant Responses to Biological and Abiotic Stresses)
    Abstract Heavy metal (HM) accumulation in soil poses a major hazard to both ecological health and plant growth progressions. Cadmium (Cd), lead (Pb), copper (Cu), chromium (Cr), arsenic (As), zinc (Zn), and nickel (Ni) are examples of HMs that negatively impact the growth and development of plants, resulting in lower agricultural output and food safety concerns. Biochar (BC), a substance rich in carbon that is formed by pyrolyzing natural biomass, has demonstrated remarkable promise in reducing HM stress in polluted soils. Research has shown that BC effectively lowers plant uptake of metals, and enhances soil qualities,… More >

  • Open AccessOpen Access

    ARTICLE

    Impact of Drought, Salinity, and Waterlogging on Wheat: Physiological, Biochemical Responses, and Yield Implications

    Mudasser Mehmood1,*, Zoahaib Aslam Khan1, Adil Mehmood2, Madiha Zaynab3, Muhammad Atiq ur Rahman4, Mohammad Khalid Al-Sadoon5, M. Harshini6, Ling Shing Wong7
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1111-1135, 2025, DOI:10.32604/phyton.2025.059812 - 30 April 2025
    (This article belongs to the Special Issue: Abiotic Stress Tolerance in Crop Plants: Physio-biochemical and Molecular Mechanisms)
    Abstract Wheat (Triticum aestivum L.) is a staple crop critical for global food security, yet its productivity is significantly affected by abiotic stresses such as drought, salinity, and waterlogging, which are exacerbated by climate change. This study evaluated the effects of these stresses on vegetative growth, physiological responses, and yield. Field experiments were conducted using a Randomized Complete Block Design (RCBD) at the Mona Reclamation Experimental Project (MREP), WAPDA, Bhalwal, Sargodha, Punjab Pakistan. Stress treatments included three levels of drought (25%, 50%, and 75% field capacity), salinity (4, 8, and 12 dS/m), and waterlogging (24, 48, and… More >

  • Open AccessOpen Access

    ARTICLE

    Metabolomic and Transcriptomic Insights into Enhanced Paclitaxel Biosynthesis in Cultivated Taxus cuspidata

    Dandan Wang*, Jiaxin Chen, Yanwen Zhang
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1137-1158, 2025, DOI:10.32604/phyton.2025.063894 - 30 April 2025
    (This article belongs to the Special Issue: Advances in Molecular Insights of Plant Secondary Metabolites: Biosynthesis, Regulation, and Applications)
    Abstract Taxus cuspidata, a rare species of the Taxus genus, and its wild resources are under severe threat. The development of cultivated species has become an important strategy to replace wild species. The objective of this work was to elucidate the differences in secondary metabolite accumulation, particularly in the paclitaxel biosynthesis pathway, between wild and cultivated species. This study employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) and RNA sequencing (RNA-Seq) technologies to conduct integrated metabolomic and transcriptomic analyses of wild and cultivated species of T. cuspidata. The results showed that the content of paclitaxel in cultivated species was significantly higher… More >

  • Open AccessOpen Access

    ARTICLE

    Identification of PBL Gene Family in Tree Peonies and Its Function in Regulating Pollen Tube Growth

    Yuxin Zhao, Zhanxiang Tan, Yuying Li, Kaiyue Zhang, Lili Guo*, Xiaogai Hou*
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1159-1176, 2025, DOI:10.32604/phyton.2025.063737 - 30 April 2025
    (This article belongs to the Special Issue: Ornamental Plants: Traits, Flowering, Aroma, Molecular Mechanisms, Postharvest Handling, and Application)
    Abstract Receptor-like cytoplasmic kinases (RLCKs) play a crucial role in the physiological processes of plant growth and development and stress response. To elucidate the characteristics and functions of the PBL gene family in tree peonies, the whole genome identification of PBL family members in tree peonies was conducted using a bioinformatics approach based on the published Arabidopsis thaliana PBL protein sequence. A total of 51 PoPBL members were identified, which were distributed unevenly on five chromosomes in the tree peony. PoPBL proteins were localized in the nucleus, cytoplasm, chloroplasts, and mitochondria, with most members of the same clade… More >

  • Open AccessOpen Access

    REVIEW

    Function of Biochar: Alleviation of Heat Stress in Plants and Improvement of Soil Microbial Communities

    Arpita Rani Roy1, Israt Jahan2, Sharah Jabeen Mou2, Md. Farhan Hasin3, Prodipto Bishnu Angon1,*, Rebeka Sultana2, Badhon Mazumder4, Md. Arif Sakil5,*
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1177-1210, 2025, DOI:10.32604/phyton.2025.063675 - 30 April 2025
    (This article belongs to the Special Issue: Soil Microbes and Abiotic Stress Factors: Impacts on Root Physiology, Crop Growth, and Hormonal Dynamics)
    Abstract Global warming is making plants more susceptible to heat stress. Hence, adjustments to crop production systems are required for global food security. Heat stress (HS) poses a threat to the quality of ecosystems and global food security due to its adverse effects on plant development. The degree to which HS affects physiological disruptions, physical harm, and biochemical changes at various growth stages directly correlates with its effects on physiological functions, plant growth, and crop production. One promising approach is soil modification using biochar, which enhances soil health and promotes the development of microbial communities, ultimately… More >

  • Open AccessOpen Access

    ARTICLE

    Molecular Cloning, Subcellular Localization and Expression Analyses of PdbHLH57 Transcription Factor in Colored-Leaf Poplar

    Yuhang Li1, Li Sun1, Tao Wang1, Bingjun Yu2, Zhihong Gao3, Xiaochun Shu1, Tengyue Yan1, Weibing Zhuang1,2,*, Zhong Wang1,*
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1211-1223, 2025, DOI:10.32604/phyton.2025.063647 - 30 April 2025
    Abstract bHLH transcription factors, widely exist in various plants, and are vital for the growth and development of these plants. Among them, many have been implicated in anthocyanin biosynthesis across various plants. In the present study, a PdbHLH57 gene, belonging to the bHLH IIIf group, was characterized, which was isolated and cloned from the colored-leaf poplar ‘Zhongshancaiyun’ (ZSCY). The cDNA sequence of PdbHLH57 was 1887 base pairs, and the protein encoded by PdbHLH57 had 628 amino acids, the isoelectric point and molecular weight of which were 6.26 and 69.75 kDa, respectively. Through bioinformatics analysis, PdbHLH57 has been classified… More >

  • Open AccessOpen Access

    ARTICLE

    Antibacterial, Antioxidant, and Anti-Inflammatory Activities of Artemisia dracunculus Essential Oil: Estragole as the Major Bioactive Compound

    Ammar Attar*
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1225-1237, 2025, DOI:10.32604/phyton.2025.063207 - 30 April 2025
    (This article belongs to the Special Issue: Innovative Approaches in Experimental Botany: Essential Oils as Natural Therapeutics)
    Abstract Artemisia dracunculus L., or tarragon, is a perennial herb from the Asteraceae family that is extensively cultivated for its aromatic leaves, which are valued for its preventative and therapeutic properties in both cookery and traditional medicine. This study aims to investigate the antibacterial, antioxidant, and anti-inflammatory properties of A. dracunculus (tarragon) essential oil (ADEO), with estragole (57.23%) identified as the major compound through gas chromatography-mass spectrometry (GC-MS) analysis. ADEO exhibited varying degrees of antibacterial activity, with Escherichia coli showing higher resistance (inhibition zone (IZ) = 14.7 ± 0.58 mm, minimum inhibitory concentration (MIC) = 2% and minimum bactericidal… More >

  • Open AccessOpen Access

    ARTICLE

    Aphicidal and Antimicrobial Activities of Salvia rosmarinus Essential Oil and Its Major Compound, 1,8-Cineole

    Ghizlane Houzi1, Aimad Allali2,3,*, Amine Elbouzidi4,*, Mohamed Taibi4, Mohamed Chebaibi3,5, Ben Khada Zineb6, Ramzi A. Mothana7, Mohammed F. Hawwal7, Rachid Flouchi3,8, Abdeslam Asehraou9, Amal Lahkimi2, Soad Khal-Layoun1
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1239-1251, 2025, DOI:10.32604/phyton.2025.063021 - 30 April 2025
    (This article belongs to the Special Issue: Biological Activities of Essential Oils)
    Abstract This work uses GC-MS to analyze the bioactive compounds of Salvia rosmarinus essential oils (SREO) and evaluates their antibacterial, antifungal, and insecticidal effects, as well as the major component, 1,8-cineole. Chemical analysis identified 16 compounds accounting for 99.19% of the oil’s total content, with 1,8-cineole (33.17%), camphor (16.53%), α-pinene (14.46%), and camphene (8.14%) as the major constituents. Antimicrobial activities were assessed against pathogenic strains using minimal inhibit concentration (MIC) and minimum bactericidal concentration (MBC) assays. SREO exhibited a minimum MIC of 0.128% against P. aeruginosa, while 1,8-cineole showed a minimum MIC of 2.06% against the same strain,… More >

  • Open AccessOpen Access

    ARTICLE

    Optimizing Ethanol Concentrations to Enhance Growth and Bioactive Compound Production in Agastache rugosa Cultivated in a Plant Factory

    Vu Phong Lam1,2,#, Dao Nhan Loi2,3,#, Xin Wang3, Jongwon Do4,5, Isaya Kisekk5, Nguyen Thi Thanh Nga6, Jongseok Park1,3,*
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1253-1267, 2025, DOI:10.32604/phyton.2025.062507 - 30 April 2025
    (This article belongs to the Special Issue: Advances in Molecular Insights of Plant Secondary Metabolites: Biosynthesis, Regulation, and Applications)
    Abstract Agastache rugosa, a medicinal plant known for its bioactive compounds, has gained attention for its pharmacological and commercial potential. This study aimed to optimize ethanol concentration to enhance growth and bioactive compound production in A. rugosa cultivated in a controlled plant factory system. Ethanol treatments at 40 and 80 mM significantly promoted both vegetative and reproductive growth. Plants treated with these concentrations exhibited higher net photosynthetic rates (A) and intercellular CO2 concentration (Ci) compared to the untreated control, whereas stomatal conductance (gs) and transpiration rate (E) remained unaffected. Chlorophyll and carotenoid concentrations, and SPAD values, significantly increased with ethanol… More >

  • Open AccessOpen Access

    ARTICLE

    Reprogramming the Metabolome of Centella asiatica (L.) Urban Callus: Profiling of Newly Synthesized Cryptic Anthocyanins Triggered by LED Light Exposure

    Claude Y. Hamany Djande, Paul A. Steenkamp, Ian A. Dubery*
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1269-1286, 2025, DOI:10.32604/phyton.2025.062392 - 30 April 2025
    (This article belongs to the Special Issue: Advances in Molecular Insights of Plant Secondary Metabolites: Biosynthesis, Regulation, and Applications)
    Abstract Centella asiatica L., a medicinal herb, has attracted substantial interest in research as well as commercial domains due to its bioactive compounds which include the pentacyclic triterpenoid centellosides, and in addition, hydroxy. In addition, hydroxycinnamic acid conjugates as well as flavonoids. The latter is the major class of secondary plant metabolites and comprises various subclasses, including anthocyanidins. Anthocyanins are rarely reported in extracts from C. asiatica and differ structurally due to a flavylium (2-phenylchromenylium) ion that carries a positive charge at the oxygen atom of the C-ring of the basic flavonoid structure. Callus of C. asiatica was initiated… More >

  • Open AccessOpen Access

    ARTICLE

    Effects of Drought Stress on the Physiological Characteristics of Flue-Cured Tobacco during the Vigorous Growing Period

    Kesu Wei1, Guangju Liu2, Bin Wei3, Qifang Zhang4, Shengjiang Wu1, Zhaowei Li2,*
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1287-1298, 2025, DOI:10.32604/phyton.2025.062385 - 30 April 2025
    (This article belongs to the Special Issue: Abiotic Stress in Agricultural Crops)
    Abstract To systematically examine the tolerance of flue-cured tobacco K326 to soil drought stress, this study set up different water content trials for K326 at 60%, 40%, and 20% levels during the vigorous growing period by greenhouse pot planting. Pigment content, chlorophyll fluorescence parameters, antioxidant enzyme activity, and SPS and GS activities associated with carbon and nitrogen metabolism were investigated in the K326 tobacco leaves. The results showed that chlorophyll a and chlorophyll b contents decreased, non-photochemical quenching coefficient Y(NPQ) decreased in the later stage of vigorous growth, and energy dissipation quantum yield Y(NO) levels increased… More >

  • Open AccessOpen Access

    ARTICLE

    Drought-Ready Soybean Resilience: Molecular Marker-Based Screening and Selection of Superior Genotypes

    Muhammad Imran Khan1, Fahad Masoud Wattoo1, Kamran Shehzad Bajwa2,*, Zahid Akram1, Sabahat Noor2, Zaheer Abbas2, Rashid Mehmood Rana1, Muhammad Tahir Akram3, Shaukat Ali2, Rai Muhammad Amir4, Ghulam Muhammad Ali5
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1299-1318, 2025, DOI:10.32604/phyton.2025.062323 - 30 April 2025
    (This article belongs to the Special Issue: Advances in Enhancing Grain Yield: From Molecular Mechanisms to Sustainable Agriculture)
    Abstract The agricultural sector is notably affected by climate change, especially soybeans, which may face diminished yields because of severe water shortages. The evaluation of germplasm at morphological and molecular levels is an important pre-breeding step for crop improvement. This study employed 10 simple sequence repeat (SSR) markers to examine 60 soybean genotypes in the quest for drought-resistant lines during 2022–23. The results of the screening experiment (PEG-6000) revealed that the soybean genotypes SPS13, SPS195, PGRB83, and 39982 exhibited significant correlations in growth parameters. The results of molecular characterization indicated that five out of ten molecular… More >

  • Open AccessOpen Access

    ARTICLE

    Enhancing Salt Stress Tolerance in Portulaca oleracea L. Using Ascophyllum nodosum Biostimulant

    Zahra Alhawsa*, Rewaa Jalal, Nouf Asiri
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1319-1337, 2025, DOI:10.32604/phyton.2025.061918 - 30 April 2025
    (This article belongs to the Special Issue: Abiotic Stress in Agricultural Crops)
    Abstract Salinity stress is a major constraint on agricultural productivity, particularly in arid and semi-arid regions. This study evaluated the potential of Ascophyllum nodosum extract (ANE) in mitigating salinity-induced stress and enhancing the growth and physiological performance of Portulaca oleracea L. under NaCl concentrations of 0, 50, 70, and 100 mM for 50 days. A two-way ANOVA assessed the effects of NaCl concentration, ANE treatment, and their interaction. The results showed that ANE significantly increased plant height at 50 mM NaCl (p = 0.0011) but had no effect at higher salinity levels (p > 0.05). Shoot dry weight was… More >

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